EMAGE mouse gene expression spatial database

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EMAGE:10999

Cend1 cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001260. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001260_01	euxassay_001260_02	euxassay_001260_03	euxassay_001260_04

euxassay_001260_05	euxassay_001260_06	euxassay_001260_07	euxassay_001260_08	euxassay_001260_09

euxassay_001260_10	euxassay_001260_11	euxassay_001260_12	euxassay_001260_13	euxassay_001260_14

euxassay_001260_15	euxassay_001260_16	euxassay_001260_17	euxassay_001260_18	euxassay_001260_19

euxassay_001260_20	euxassay_001260_21	euxassay_001260_22	euxassay_001260_23	euxassay_001260_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10999_wholemount_strong.wlz

10999_wholemount_moderate.wlz

10999_wholemount_weak.wlz

10999_wholemount_possible.wlz

10999_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10999_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 12 13 14 15 17 18 19 20

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 10 11 12

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 11 15 16

pons mantle layer

moderate

regional

moderate expression: see section 06 07 14 17

midbrain mantle layer

moderate

regional

moderate expression: see section 07 10 11 12 13 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 18 19

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

ventral grey horn

strong

regional

strong expression: see section 09 10 11 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 11 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 15 16 17

cornea stroma

moderate

regional

moderate expression: see section 02 03 04 05 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 15 18 19 20 weak expression: see section 10 11 12 16 17 21

urethra of male

strong

regional

strong expression: see section 17 moderate expression: see section 13 weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5408
Entity Detected:	Cend1, cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
Sequence:	sense strand is shown >T5408 GAAGCGAATGCGCAGCGAGAAGCTGGAGGGCCCAAAGAAGACACCCATATAATGCCCAGAGGTGAGCTGT CCTGAGCCACTGTGGTCTAGCCACCTGCCAGTCACACAAGCCCCAAACCAGCTGCCAGCAAAAAACCATC ATGGAATCCCGAGGAAAGTCAGCCAGCAGCCCCAAGCCAGATACCAAGGTGCCTCAGGCCACAGCTGAGG CCAAGGCCACTCCAGCTGCGGATGGGAAAGCTCCCTTGACCAAGCCTGTGAAGAAGGACACCCAAGCAGA GAAGCAGGAGCAAGCAGCAGCCCCTGGGCCAGCCGCTACGAAGAAGACACCAGCCAAGGCAGATCCTGTA CTTCTCAACAACCACAGCAACCTGAAGCCAGCTCCCACAGTGCCGGCAGCCCCCAGCAGTCCTGATGCAA CCTCAGAGCCCAAGGGTCCTGGGGATGGGGCAGAGGAGGACGAGTCCAACACTGGAGGCCGAGGTCCCTG GCCTTGTGAGAACTTGACCCCTTTGCTGGTAGCTGGGGGTGTGGCCGTGGCCACCATAGCTCTAATTCTT GGTGTGGCCTTTCTAGCCCGGAAAAAATGATACCTGGTGTCCAGGTCGGGGCACAGAGCCACTTTCTGTA CAGACCTGAATATATAGTGCATGCAAGGTCCTAGATATTTATCTATACATATACACCTAACAACACACAC ACAACACACACACACACTCACACGTATATGTATGCTACATACATCCGTGCAGCAGGAAAGAAATAGACAG TGAGCCCACCTCCACTCACCCCAGCACACCCTCTCCCAAGTTCTGGGGCAGGAGCCCAGCTCTGCAGTTT GCAGGAAGCACTAGCGTGGGGCAAAGGGGAGAGATGCTGGGTGTGGTCTCTGAAAGATAAGTGATGGAGT GCGCTAGCCCTGTGGGGACATTAAAGGGCATGGCTGTCTTACTCCTCTCACTTGTCTTTTGAGCAGCCAG GAGCAGTCTGTCTGGGCCTGATGCCGGGGGACTGACCTAAGGGTGGGTGGGATTCAAATCTGCAGAGCTA CCTCTACCCATTGTCCCTGTCCTGGTTGGATGGAAAAGGCAAAGTTAACAGCTGAGTTTGGGATGGGGTT CCCACCTCTTCCCTCTGTCAGTGTATGCCCCAGGAGAGCACCGGAGGCTAGGACCACCTTACTTCCTGCC CAGAGCAGCCAATTGTTCCGGGGAGGGGGTGGTTCTGAAAGCCTGGCAGGGAGGCAGGAAGCACGAAGGA GCCACACTACAGTTCTGCCTGCCCAATATTCCCCAATACTGTCCTTCCTGCCTGCACCCAACACCCCCCC CCTTGAGTTTAACAGCGTATAAATGCAATAACACAGGTAGAGTAGAACTGCCCGGTGAGTGGCAATCGCT CACTGCCTGCAGGAAGACCTGCTGTGCAGCCCCTCTTTAGTGCATCTGGCCCCAGCTTGCCCCTTGGTGT GGTGACAGTGAGCAGCTTTCCGGAAAACTGCAGTGGGCTCTGAGGAAACATTGCCCTCACCTGGCCAGTG GCGGCGGGGACCTTGGGCCGGAGTGGAGACTGAGTTTGAAATAAACCGTTACAATATAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5366891 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366891 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11000 same embryo
	EMAGE:10997 same embryo
	EMAGE:10996 same embryo
	EMAGE:10998 same embryo
	EMAGE:11001 same embryo
	EurExpress:euxassay_001260 same experiment
	MGI:4823806 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source