EMAGE mouse gene expression spatial database

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EMAGE:11004

Mapkapk3 mitogen-activated protein kinase-activated protein kinase 3 ( MGI:2143163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001044. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001044_01	euxassay_001044_02	euxassay_001044_03	euxassay_001044_04

euxassay_001044_05	euxassay_001044_06	euxassay_001044_07	euxassay_001044_08	euxassay_001044_09

euxassay_001044_10	euxassay_001044_11	euxassay_001044_12	euxassay_001044_13	euxassay_001044_14

euxassay_001044_15	euxassay_001044_16	euxassay_001044_17	euxassay_001044_18	euxassay_001044_19

euxassay_001044_20	euxassay_001044_21	euxassay_001044_22	euxassay_001044_23	euxassay_001044_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11004_wholemount_strong.wlz

11004_wholemount_moderate.wlz

11004_wholemount_weak.wlz

11004_wholemount_possible.wlz

11004_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11004_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

homogeneous

weak expression: see section 08 09 10 16 17 18

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

foregut-midgut junction

weak

regional

weak expression: see section 13 14

vibrissa

moderate

regional

moderate expression: see section 06 07 22 23

ventral grey horn

moderate

regional

moderate expression: see section 12 13

external naris

moderate

regional

moderate expression: see section 12 14 17 18 weak expression: see section 13

esophagus

weak

regional

weak expression: see section 12 13 14

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 weak expression: see section 08 10

hindgut

weak

regional

weak expression: see section 12 13 14

midgut

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 16 17 18

oral epithelium

weak

regional

weak expression: see section 07 08 10 11 12 13 14 15 16 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 13 16 17 18

liver lobe

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

weak

regional

weak expression: see section 13 14

kidney calyx

weak

spotted

weak expression: see section 07 08 19 20

kidney pelvis

weak

spotted

weak expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1056
Entity Detected:	Mapkapk3, mitogen-activated protein kinase-activated protein kinase 3 ( MGI:2143163)
Sequence:	sense strand is shown >T1056 TCGAGNCTGNNTGGCCTACTGGGAATATGCACCACGGCAAGCGCTGTCTCCTCATTGTCATGGAATGCAT GGAGGGTGGTGAGCTGTTCAGCAGGATTCAGGAGCGTGGTGACCAGGCTTTCACTGAGAGAGAGGCTGCA GAGATAATGCGGGACATTGGCACTGCCATCCAGTTCTTGCACAGCCGGAACATTGCCCACCGAGATGTCA AGCCTGAAAACCTACTCTATACATCCAAGGAGAAGGGTGCTGTACTTAAACTCACCGATTTTGGCTTTGC CAAGGAAACCACCCAAAATGCCCTCCAGACACCCTGCTACACTCCCTATTATGTGGCTCCTGAGGTCCTG GGTCCAGAGAAGTATGACAAGTCCTGTGATATGTGGTCCCCTGGCGTCATCATGTACATCCTTTTGTGTG GATTCCCACCCTTCTACTCCAACACCGGCCAGGCCATCTCTCCAGGAATGAAAAGAAGGATTCGCTTGGG CCAGTATAGCTTCCCTAACCCTGAATGGTTAGATGTCTCTGAGGATGCCAAGCAGCTAATCCGCCTGCTC CTGAAGACA
Notes:	The probe template was PCR amplified from IMAGE:2088451 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088451 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11005 same embryo
	EMAGE:11003 same embryo
	EMAGE:11002 same embryo
	EMAGE:11006 same embryo
	EurExpress:euxassay_001044 same experiment
	MGI:4826097 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source