EMAGE mouse gene expression spatial database

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EMAGE:11017

Lims1 LIM and senescent cell antigen-like domains 1 ( MGI:1195263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003410. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003410_01	euxassay_003410_02	euxassay_003410_03	euxassay_003410_04

euxassay_003410_05	euxassay_003410_06	euxassay_003410_07	euxassay_003410_08	euxassay_003410_09

euxassay_003410_10	euxassay_003410_11	euxassay_003410_12	euxassay_003410_13	euxassay_003410_14

euxassay_003410_15	euxassay_003410_16	euxassay_003410_17	euxassay_003410_18	euxassay_003410_19

euxassay_003410_20	euxassay_003410_21	euxassay_003410_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11017_wholemount_strong.wlz

11017_wholemount_moderate.wlz

11017_wholemount_weak.wlz

11017_wholemount_possible.wlz

11017_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11017_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 16 17

gut

moderate

regional

moderate expression: see section 01 06 07 08 09 10 11 12 13 14 15 16 17

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 19 20 21 22

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

4th ventricle

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

heart

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

tongue

moderate

regional

moderate expression: see section 12 13 14 15 16

stomach

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 18 19

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 18 19

liver and biliary system

strong

single cell

strong expression: see section 01

liver

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

bladder

moderate

regional

moderate expression: see section 12 13 14 15 16

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19

lung

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6825
Entity Detected:	Lims1, LIM and senescent cell antigen-like domains 1 ( MGI:1195263)
Sequence:	sense strand is shown >T6825 GCGGCGGGGACTGAGGCACAGACTGACCCGGCGTGGACCGCGGAGATGCTGGGCGTGGCGGCCGGAATGA CCAACAGCAACATGGCCAATGCCTTGGCCAGTGCCACGTGTGAGCGCTGCAAGGGAGGCTTTGCGCCTGC TGAGAAGATCGTGAACAGTAATGGTGAGCTGTACCACGAACAGTGCTTCGTATGTGCTCAGTGCTTCCAG CAGTTCCCAGAAGGACTCTTTTATGAGTTTGAAGGAAGGAAGTACTGTGAGCATGATTTCCAAATGCTCT TTGCTCCCTGCTGCCACCAATGTGGTGAATTCATCATTGGCAGGGTTATCAAAGCCATGAACAACAGCTG GCATCCTGAGTGCTTCCGATGTGACCTCTGCCAGGAAGTGCTGGCAGACATAGGGTTTGTCAAGAATGCT GGCAGACACCTATGTCGCCCATGTCATAATCGTGAAAAAGCCAGAGGCCTTGGAAAATACATCTGCCAGA AATGCCATGCCATCATTGATGAACAGCCTCTGATCTTCAAGAACGACCCTTACCATCCAGACCACTTCAA CTGTGCCAACTGCGGGAAGGAGCTAACTGCTGATGCTCGGGAGCTGAAAGGGGAGCTATACTGTCTGCCA TGCCATGATAAGATGGGGGTCCCTATCTGTGGCGCCTGCAGGAGGCCCATTGAAGGGCGAGTAGTGAATG CCATGGGCAAGCAATGGCATGTGGAGCATTTTGTTTGTGCCAAATGTGAAAAACCATTTCTTGGGCATCG CCATTATGAGAGGAAGGGCTTGGCCTACTGTGAAACTCACTATAATCAGCTGTTTGGTGATGTTTGTTTC CATTGCAACCGTGTCATAGAAGGTGATGTGGTCTCTGCTCTCAACAAGGCCTGGTGTGTGAGCTGCTTTG CCTGCTCCACCTGCAATACCAAACTCACACTCAAGAATAAATTTGTGGAATTTGACATGAAGCCAGTCTG TAAGAAGTGCTACGAGAAATTTCCTTTGGAGCTGAAGAAAAGACTTAAGAAACTATCTGAGACCTTAGGA AGGAAATAACTTTTGGTGTCTTTTTTCTCTCTCTTCTAATCAATGTTACTTAACCTGATCTACCCTTGCT TAAAACTATGTCAGAGCATTAGAATGGTTTTCTTCATTTCCCTTTTCTCATTTAAAAACGAAAGAAAATG TTTATTTCTATGTAAAACACAGATGAAATCAGGACTTTCATTTCTTTAGCCATATTAGTCTGTAGGCATT AAAAGTATATAACCAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3711611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11013 same embryo
	EMAGE:11014 same embryo
	EMAGE:11016 same embryo
	EMAGE:11015 same embryo
	EurExpress:euxassay_003410 same experiment
	MGI:4825929 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source