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EMAGE:11068

Wisp1 WNT1 inducible signaling pathway protein 1 ( MGI:1197008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068
"Pseudo-wholemount" of euxassay_006558. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006558_01 euxassay_006558_02 euxassay_006558_03 euxassay_006558_04
EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068
euxassay_006558_05 euxassay_006558_06 euxassay_006558_07 euxassay_006558_08 euxassay_006558_09
EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068
euxassay_006558_10 euxassay_006558_11 euxassay_006558_12 euxassay_006558_13 euxassay_006558_14
EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068
euxassay_006558_15 euxassay_006558_16 euxassay_006558_17 euxassay_006558_18 euxassay_006558_19
EMAGE:11068 EMAGE:11068 EMAGE:11068 EMAGE:11068
euxassay_006558_20 euxassay_006558_21 euxassay_006558_22 euxassay_006558_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11068Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11068_wholemount_strong.wlz
11068_wholemount_moderate.wlz
11068_wholemount_weak.wlz
11068_wholemount_possible.wlz
11068_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11068_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23
diaphragm
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
forearm mesenchyme
strong strong
regionalstrong expression: see section 01 02
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 20 21 22 23
hand
strong strong
regionalstrong expression: see section 01 02 03 04 05 06
foot
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 23
lower leg mesenchyme
strong strong
regionalstrong expression: see section 01 03 04 05 06 07
fibula
strong strong
regionalstrong expression: see section 02
tibia
strong strong
regionalstrong expression: see section 02
upper leg mesenchyme
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 20 21 22 23
femur
strong strong
regionalstrong expression: see section 02 20
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 20 21 22 23
diencephalon meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
diencephalon roof plate
strong strong
regionalstrong expression: see section 14 moderate expression: see section 07 08 09 10 11 12 13 15 16 17
telencephalon meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 03 04 21 22 23
hindbrain meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 03 04
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
trigeminal v ganglion
strong strong
single cellstrong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22
spinal cord meninges
strong strong
regionalstrong expression: see section 08 09 10 11 12 16 17 18 moderate expression: see section 13 14 15
aorta
strong strong
regionalstrong expression: see section 11 12 13 14 15
heart atrium
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
stomach
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 moderate expression: see section 01 02 03 04 05
midgut
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
mandible
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
maxilla
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23
urinary system
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 10 11 12
metanephros
strong strong
regionalstrong expression: see section 08 09 18 19 20 21 moderate expression: see section 10 11
axial skeleton
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
basisphenoid bone
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16
vault of skull
strong strong
regionalstrong expression: see section 01 02 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 22 23
clavicle
strong strong
regionalstrong expression: see section 08 09 10 17 18 19
scapula
strong strong
regionalstrong expression: see section 03 04 05 06 20 21 22 23
sternum
strong strong
regionalstrong expression: see section 15 16
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 10 11 12 19
tail paraxial mesenchyme
strong strong
regionalstrong expression: see section 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8081
Entity Detected:Wisp1, WNT1 inducible signaling pathway protein 1 ( MGI:1197008)
Sequence:sense strand is shown

>T8081
AAGAGGTAATCCTTGACAAACGCCCCCGGGGGCTGAGGAAGGCTCTCCGCTGCTCTGATGGGCCAGCCCA
GTCGTGGCCCAGCTCCCTGGAGAGGCATCCGCATCCTCTGGGCTGAGCCGTAGCTCCTGTGACGCTGACT
TCCAGGCATGAGGTGGCTCCTGCCCTGGACGCTGGCAGCCGTGGCAGTCCTGAGGGTGGGCAACATCCTG
GCCACGGCCCTCTCTCCAACCCCCACAACAATGACCTTCACCCCAGCACCACTAGAGGAAACGACTACAC
GCCCCGAATTCTGCAAGTGGCCATGTGAGTGCCCACAATCCCCACCTCGCTGCCCACTGGGCGTCAGCCT
AATCACAGATGGCTGTGAATGCTGTAAGATACGTGCCCAGCAGCTTGGGGACAACTGCACAGAGGCTGCC
ATCTGTGACCCACACCGGGGCCTCTACTGCGATTACAGTGGGGATCGCCCGAGGTACGCAATAGGAGTGT
GTGCACAGGTGGTCGGTGTGGGCTGTGTCCTGGATGGCGTACGCTACACCAATGGCGAGTCCTTCCAACC
CAACTGCAGGTACAACTGTACCTGCATTGATGGCACGGTGGGCTGCACACCGCTGTGCCTAAGCCCCAGG
CCCCCACGCCTCTGGTGCCGCCAGCCCCGGCACGTGAGAGTCCCTGGCCAGTGCTGTGAGCAGTGGGTGT
GTGATGATGACGCAAGGAGACCACGCCAGACTGCACTGTTGGGCACCAGAGCCTTTGCAGCGTCAGGCGC
CGTGGAGCAACGGTATGAGAACTGCATAGCCTACACTAGTCCCTGGAGCCCCTGCTCTACCACCTGTGGC
CTAGGTATCTCCACTCGGATCTCTAACGTCAATGCCCGGTGCTGGCCAGAGCAGGAAAGTCGCCTCTGCA
ACCTGCGGCCATGTGATGTGGACATCCAACTACACATCAAGGCAGGGAAGAAATGCCTGGCTGTGTACCA
GCCAGAGGAGGCCACGAACTTCACTCTCGCAGGCTGTGTCAGCACACGCACCTACCGACCCAAGTACTGC
GGAGTCTGTACTGACAATAGGTGTTGCATCCCCTACAAGTCCAGGACCATCAGTGTGGATTTCCAGTGTC
CAGAGGGGCCAGGTTTCTCCCGGCAGGTCCTATGGATTAATGCTTGCTTCTGCAACCTGAGCTGCAGGAA
TCCTAACGATATCTTTGCTGACTTGGAATCTTACCCTGACTTCGGAGAGATTGCCAATTAGGTGGGTGTG
TGGCTCAGGGTAAAGTTCCATGCTGCAAAGCAGCCAGCCCTTTGTGGTCCAGGACTTCACAATTGAGCCT
TATTTCATCTACTTCCTACTCGATTCTGAATTCCCAGTTTCTGTTCCTGTTTTGACAATCGTAATGGCCC
AGGAGAGTGCTGCTCAGGCTCAGACAATGGGTTCCTCCTTGGGGACATTCTACATCATTCCAAGGAAAAC
ACATCTCTGACTGTTCACAATGGAAGCAAAGCCTGGCCCAGCTAGTCTGGCTCCAGCCTGGGCAAGTTGT
CAGAAGTTGTGATGGGATTGTCCAAGGAAAAGCATCAGCTGAAGAACCAGTATCATGAAGTCCTTCCTCA
GATGCCAAGCCTAGGGATGCTGGGATCCTTTCAGACAGATGGATGGGATTGGGGACACAGGAATAAGCTA
TTATTTTACCCTTGCCAAATGATACTATCCTGGGTATTTCTGCCTAAAACATACCAAAAGTGTTCTTGTT
CCACTGATCTGTATATCACAAGTCACCAAACATTTTCCAGGTGAGGACCATAGTTGTGTCATTCTGTTTT
GCTATTGAAAATCATTTTAAAAAGAAGGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30062053 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30062053 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11070 same embryo
 EMAGE:11069 same embryo
 EMAGE:11072 same embryo
 EMAGE:11071 same embryo
 EurExpress:euxassay_006558 same experiment
 MGI:4829219 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS