EMAGE mouse gene expression spatial database

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EMAGE:11072

Ung uracil DNA glycosylase ( MGI:109352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006557. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006557_01	euxassay_006557_02	euxassay_006557_03	euxassay_006557_04

euxassay_006557_05	euxassay_006557_06	euxassay_006557_07	euxassay_006557_08	euxassay_006557_09

euxassay_006557_10	euxassay_006557_11	euxassay_006557_12	euxassay_006557_13	euxassay_006557_14

euxassay_006557_15	euxassay_006557_16	euxassay_006557_17	euxassay_006557_18	euxassay_006557_19

euxassay_006557_20	euxassay_006557_21	euxassay_006557_22	euxassay_006557_23	euxassay_006557_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11072_wholemount_strong.wlz

11072_wholemount_moderate.wlz

11072_wholemount_weak.wlz

11072_wholemount_possible.wlz

11072_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11072_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 14 15 moderate expression: see section 12 13

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 20 moderate expression: see section 07 17 18 19

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

thyroid gland

strong

regional

strong expression: see section 10 11 moderate expression: see section 15 16

vibrissa

strong

regional

strong expression: see section 09 moderate expression: see section 07 08 10 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 14 15 moderate expression: see section 12 13

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 24

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 09 14 15 16 17 18 19 20 moderate expression: see section 04 05 06 07

rest of cerebellum marginal layer

strong

regional

strong expression: see section 18 19 20 21 moderate expression: see section 03

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 08 09 10 11 15 16 17 18 19 20 21 moderate expression: see section 03 04 05 06 07 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 04 05 06

midbrain ventricular layer

strong

graded

strong expression: see section 07 08 09 10 11 12 13 14 15 moderate expression: see section 06

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 16 17 18 19 20 21

stomach

strong

regional

strong expression: see section 09 10 moderate expression: see section 02 03 04 05 06 07 08

rectum

strong

regional

strong expression: see section 15

midgut

strong

regional

strong expression: see section 09 10 13 14 15 16 17 18 moderate expression: see section 11 12 19 20

lower jaw incisor

strong

regional

strong expression: see section 13 14 17 18

lower jaw molar

strong

regional

strong expression: see section 07 08 09 20 21

upper jaw incisor

strong

regional

strong expression: see section 13 14 15 17 18 19

upper jaw molar

strong

regional

strong expression: see section 07 08 20 21

renal cortex

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8082
Entity Detected:	Ung, uracil DNA glycosylase ( MGI:109352)
Sequence:	sense strand is shown >T8082 AAGAGGTAATCCGGACCATGGGCGTCTTGGGGCGGCGGTCTTTGCGGTTGGCGCGGAGAGCGGGGCTCCG GAGCCTGACTCCCAATCCCGACTCTGACTCCCGGCAGGCCAGCCCCGCCAAGAAGGCCCGGGTGGAGCAG AACGAGCAAGGCTCGCCGCTCAGCGCCGAGCAGCTCGTCCGCATCCAGAGGAACAAGGCCGCGGCGCTGC TCAGGCTCGCCGCCCGCAACGTGCCTGCCGGCTTCGGCGAGAGCTGGAAGCAGCAGCTGTGCGGGGAGTT CGGGAAGCCGTACTTCGTCAAGCTAATGGGATTTGTTGCTGAAGAAAGGAACCACCACAAGGTCTATCCG CCCCCGGAGCAGGTGTTCACATGGACCCAGATGTGCGACATCCGAGATGTGAAGGTTGTCATTCTGGGAC AGGATCCCTATCACGGACCTAATCAAGCTCACGGGCTCTGCTTCAGTGTCCAAAGACCAGTTCCACCCCC GCCCAGTTTGGAAAACATTTTTAAAGAGCTGTCTACAGACATCGATGGTTTTGTTCATCCTGGCCATGGG GATTTGTCAGGGTGGGCCCGACAAGGTGTCCTCCTCCTCAACGCCGTCCTCACTGTCCGCGCCCACCAAG CCAATTCCCATAAGGAGAGGGGCTGGGAGCAGTTCACGGACGCGGTCGTGTCCTGGCTGAATCAGAACCT GAGTGGCCTCGTCTTCCTCCTCTGGGGCTCTTATGCTCAGAAGAAGGGCAGTGTCATTGACAGGAAGCGT CACCATGTTCTGCAGACAGCTCACCCCTCCCCGCTGTCGGTGTACAGAGGGTTCCTGGGATGCAGACATT TTTCTAAAGCCAATGAACTGCTCCAGAAGTCTGGCAAGAAGCCCATCAACTGGAAGGAGCTGTGACCCGC CCGCCCACAGGCCGCGTCTGCCCACAGCGGTGCTTTCAGCTACCGCCGCCGCCACCAAAGCTTTTGCTGG TTAAGTTCTAAGTTCCTTGTGTGAGCGGAGAAATGACACGGATAGATCTCTATAGGACCATCATGAGCCA ACCAGGACACAGAAACTGCAGCTCTGGCAGGCAGAACCTCTTGCCTGGCACTAGGTTTGAACTCTCCAGG CCCGGCCAGGTGGCTGTTGAGACACACCTACTTCTCAGACCAGCGGACACACCTTCGGTCCCGTGTGCTG CTCTGGTGTGGGAGGAGAGATCTGCACCTTTGGACCAGCAGTTTGGTGCCTGCTGCTGTCCGCTTTATCC TTTAGAGTTAGAGATTTCAAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTTCAAAAGGCC CTTTTGTCTGGAAAGGAACCCTTGGGTGTTGGTGAGTCCTGAGAAGTCTGGCTTACAACTGAATGAGCTT CCTGATCACAAAGGGGGCGGGGTTCTCCCTGGGTTCACAGTAGCCGCTCTCCACCCTGCGTCTTGCCTTT GCAGGCTCCGAAGTTGAGCGAAGCAGGAATGGGATAGATTTGTTTTTAAGTGAGGGTGGGTGGGTTTCCT GGTGCCCGTGGAGTTGTTTCCTCCTCTCTGCAGACAGCAGACTGCCGCGCTTGGTGTGTGTGGTGCTGGG GATCTAACTCCAAGGTGCTGTTTCTTAGAGGCTGAGTTCACATAAGTTGTCTTTCTGCAACACTGGAGAA CGTTATTTTTTTTTACAAGGTAGGCAGGTACAGTAATTTCTTTTTCATGCTTTTCTGAAAGACAATCTTC ACTGAGTTTACAAGCTGGATTCCGTGAGTCCAGCTGGTTAGAGCCGTGGCCTGCCTGACCAGGCTAATAA AAGATGGTTGACAATTAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30058519 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30058519 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11070 same embryo
	EMAGE:11069 same embryo
	EMAGE:11068 same embryo
	EMAGE:11071 same embryo
	EurExpress:euxassay_006557 same experiment
	MGI:4829106 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source