EMAGE mouse gene expression spatial database

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EMAGE:11087

Frk fyn-related kinase ( MGI:103265)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003660. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003660_01	euxassay_003660_02	euxassay_003660_03	euxassay_003660_04

euxassay_003660_05	euxassay_003660_06	euxassay_003660_07	euxassay_003660_08	euxassay_003660_09

euxassay_003660_10	euxassay_003660_11	euxassay_003660_12	euxassay_003660_13	euxassay_003660_14

euxassay_003660_15	euxassay_003660_16	euxassay_003660_17	euxassay_003660_18	euxassay_003660_19

euxassay_003660_20	euxassay_003660_21	euxassay_003660_22	euxassay_003660_23	euxassay_003660_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11087_wholemount_strong.wlz

11087_wholemount_moderate.wlz

11087_wholemount_weak.wlz

11087_wholemount_possible.wlz

11087_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11087_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

hindlimb

moderate

regional

moderate expression: see section 23 24

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 10 11 23 24

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 24

cornea

moderate

regional

moderate expression: see section 01 02 03 04 05

naris

moderate

regional

moderate expression: see section 15 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 14 15 16 18 19 20

tongue

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12

rectum

moderate

regional

moderate expression: see section 14 15 16

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22

mandible

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

palatal shelf

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 18 19 20 21 22 23

bladder

moderate

regional

moderate expression: see section 14 15 16

kidney calyx

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21 22

kidney pelvis

strong

regional

strong expression: see section 09 10

urethra of male

moderate

regional

moderate expression: see section 14 15 16

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 13 14

axial skeleton

moderate

regional

moderate expression: see section 13 14 15 16 weak expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6665
Entity Detected:	Frk, fyn-related kinase ( MGI:103265)
Sequence:	sense strand is shown >T6665 CCCACGCGTCCGGGGAGCGGGAAAGCTGACCTGGGAGTTCCTTTTAGATCAGACAAAAAGAAACCAGGAA GTTAGGAGGAATTATTACAGTCTGTACAGCTCAGGATGTTAAAGGCGATAACCTCCACGGCCTGGTTATT CATTAGCAAGAAGGAAAGCTCCGGAGAGAGCAGAACTCTCTCAGCTGCAGGCTTGAAGCAGGGCAGTCAG GAAGAAGCACCTTCCCACTGGACCTCCAGCTCAGTCCTCTCTTGGTTTTGCCTCAGGGCAGCAGCTACAA ACAGAAAGCCAGCTGTGAGTGTCTTGGCCAAGCCCAGATCTGCCGGAGAGAGGAGAGATCCGAAGCGGGA GGTATGGTGAGTTGCAGCTGATTTGGAAGCTGCCTGGTGGAGGACCGTGGCTCTGTACAGCTTGTGGGGA GAGGACTATCCTCTCCTACAACTACTAAGCTAGAACAACACCATGGGCAGCGTCTGTGTGAGACTCTGGG CATACCTGCAGCCTTTTCTCCCGTGCTGGTCTCAAGAGGCAGACAAGTCAGTAGTAATTGAGAATCCAGG GGCCTTCTGTCCCCCAGAGGCTCCCAGGTCACAAGAGCCCGAGAGAAGCCATGGCCAGTATTTTGTGGCT CTGTTTGATTACCAAGCACGTACTGCAGAGGACCTGAGCTTCCGTGCCGGCGACAAACTCCAAGTCTTGG ACACTTCGCATGAGGGCTGGTGGTTGGCCAGACATTTGGAGAAGAAGGGAACCGGCTTAGGTCAGCAGCT ACAGGGCTACATTCCTTCCAATTACGTGGCGGAGGACCGGAGTCTCCAGGCAGAGCCGTGGTTTTTTGGA GCAATCAAAAGAGCAGATGCAGAAAAACAACTTCTGTATTCAGAAAACCAGACGGGCGCCTTTCTAATCA GAGAGAGTGAGAGCCAGAAGGGTGACTTTTCCCTCTCAGTTTTAGATGAAGGTGTTGTAAAACACTACAG AATAAGAAGGTTGGATGAAGGTGGCTTCTTCCTCACCAGGAGGAAAGTCTTTTCAACCCTGAATGAATTC GTGAACTACTACACCACAACAAGTGACGGGCTGTGTGTCAAGCTGGAGAAGCCATGCTTAAAGATCCAGG TACCAACCCCTTTTGATTTGTCATATAAAACTGCAGACCAGTGGGAGATAGACCGCAACTCCATACAGCT TTTGAAGCGACTGGGATCTGGTCAGTTTGGAGAAGTTTGGGAAGGTCTGTGGAATAATACCACTCCAGTG GCCGTAAAAACGTTAAAACCAGGTTCAATGGATCCAAATGACTTCCTGAGGGAGGCACAGATAATGAAGA GCCTAAGACACCCAAAACTCATCCAGCTCTATGCTGTTTGCACTTTAGAAGATCCCATTTATATTATTAC AGAGTTGATGAGACATGGAAGCCTGCAAGAATATCTCCAAAATGATGGTGGGTCAAAAATCCATTTGATT CAACAGGTAGACATGGCGGCACAGGTGGCTTCTGGAATGGCCTATCTTGAGTCGCAGAACTATATTCACA GAGATCTGGCTGCAAGAAATGTCCTTGTTGGTGAACATAATATCTACAAAGTAGCAGATTTTGGACTTGC AAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAAACACGAAATAAAGCTGCCAGTGAAGTGG ACTGCACCCGAAGCCATTCGTACTAATAAATTCAGCATTAAGTCTGATGTGTGGTCTTTTGGAATCCTGC TCTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTATGACAGGTGCTCAAGTAATTCAAATGTT GAGTCAAAACTACAGACTTCCACAGCCATCTAACTGCCCACAGCAATTCTACAGCATCATGCTAGAGTGC TGGAATGTTGAGCCTAAGCAACGGCCAACATTTGAGACCCTGCATTGGAAACTTGAAGACTACTTTGAAA CAGACTGTTCCTATTCAGATACAAATAACTTCATAAACTAAACATCAAGGAAAAGAAGAAATATAATGAA GATATGAAAACGTGGGGCTCATCAGTCCAGAAATACTATCTATTCAACCAACTGTGAAATCAGCTTATTT TGACATATTCAAGTGGTAGGATGAACTCAGCCATGCATTCCATATCAAAAAGATAATCTGTGTATTTTAT TGACTAGGAAACACTGCAGGACAGTATAAGATAATGTGTCATTTTTCCTCACTGCCTGGTAAAATCAATC ACACTAAATGAAGTTATTTTTTTTCCTTTTGAGAACTCCTCACATTTCTATTTATTGTTTGAATGCCCAT CAAGAGAATCAACAGATGACAGTGTTTACTCAGTTACTGTTGTGGCATTTTCCTGTTTACTAATTAGACT GGTTATTCATTATTCCTCAAATTGCTGAATACCATCAGGCTGTTATTATGAAGGAATTTGATTGCTCTGC TGCACAGCAGGACCTGTGCTTTGAGATATTTTTTTTTTCCTCTCTTTTAAAATATCCTGTAACTACAATA ATGGTAAAGCCATGTTAAATGACTTGATTGTACTTGAAGTAATTGCACATATTTTTCCTATGCATAAGAA AATGAAGCAGCTGTTGAGAAAAGGAATTAAAATCTTTCTCATTTTGTAGAAGGAAATGATGGAGTTTGCT CTATACCTCAGTATGTGTCATTGGAGAATCATCTGCATTAGTTTTAATCTCTTAATGTCAGGAATCAGGT TGCAAAGCCGATGAATTGTTATGTATGGGAATATAGGGGAACTACCACATTGCCTACCAGGTCTGAGAAA TAGATATTAAAGTGGCTTAGAGAAGCTAACAGAGAGAGCTAGAGGATTGCTGCAGCTTTCTAAGTAATAA TAAAAAGTTTCTTTGGCATGCAGGCCTATTTTAAATTGGCTTCAAAACAGGCCAATCAGCACCTGAGGCT AGGAATAATGTCTGAATGTAGTTATGAACTGTAGCTTTTGTCTGTTAAGTCTGGATGCTTTTCAGTATCT TAAGTTTTTATAACCTTGAAGAACTTCCCTGTTTTCTTAGGTAATGTGTGTATCAAATGTTCTGCATCAC TGGATGGTTAGCAAGACTTTTCTAAAATCAGCTCCATAGATTCTGGAATCTTCTATTGTGCGGCACCGAA TGAACATTCCAAAAGGAAACTGAGTTTATCAAGTTTATTTTTCATCCTTCTTTAAGAGAGAAGGGACTAT CTAGATGCTCACAAGGATCCCCTGCAAATTAAAAAACATGCCAATTCTTTCAACTAGGAACTAGGAGGGT AACAGGGACTAACCATTAGCCATTTGGTGATGGCACTTCCCTAGCTGCATTTCTGTTACCATAGTAGAAA TCCTGAAAACAAAACAAAACAAAACAAAAACAAAAACAAAAACAAAAAACAAACAAACAAAACCAACTTA GAAGAGACAAGTTTTATTCTAGCTTACCATTCTAGGCCATTGCATCACAGCAGGAAGTCACTGTGACAAG AGAGCTTGAAAATGATTTCATCACATCCACAGTCTAAGAGGAGAAAGAGACATAACTACATGCACGCATG CATGACTTGCTTGTTTGCTTACTTGTGCTCAGGTCAATTTCTACACTCTTAAAATGTTTGGGACACCTTT GCCTAGGAAATAGCATGGCACACAATGGGCTGAGTTTTCCAGGATCAATTAACTTAATTAAGACAATTCT GTACAGACATGCCCACAGGCAAGTCTAATATAAAGAATCATTGTCAACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601246 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601246 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11088 same embryo
	EMAGE:11084 same embryo
	EMAGE:11089 same embryo
	EMAGE:11086 same embryo
	EMAGE:11085 same embryo
	EurExpress:euxassay_003660 same experiment
	MGI:4824922 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source