EMAGE mouse gene expression spatial database

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EMAGE:11117

Mmd monocyte to macrophage differentiation-associated ( MGI:1914718)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001135. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001135_01	euxassay_001135_02	euxassay_001135_03	euxassay_001135_04

euxassay_001135_05	euxassay_001135_06	euxassay_001135_07	euxassay_001135_08	euxassay_001135_09

euxassay_001135_10	euxassay_001135_11	euxassay_001135_12	euxassay_001135_13	euxassay_001135_14

euxassay_001135_15	euxassay_001135_16	euxassay_001135_17	euxassay_001135_18	euxassay_001135_19

euxassay_001135_20	euxassay_001135_21	euxassay_001135_22	euxassay_001135_23	euxassay_001135_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11117_wholemount_strong.wlz

11117_wholemount_moderate.wlz

11117_wholemount_weak.wlz

11117_wholemount_possible.wlz

11117_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11117_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09

vertebral axis musculature

weak

regional

weak expression: see section 08 10 11 12 16 18 19 20 21 22 23

foregut-midgut junction

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19

neural retina

moderate

homogeneous

moderate expression: see section 01 02 03 04 23 24

nasal capsule

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

esophagus

moderate

regional

moderate expression: see section 13 14

stomach

moderate

homogeneous

moderate expression: see section 02 03 04 05 07 08 09 10 11

hindgut

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17 18 19

midgut

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

meckel's cartilage

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 18 19 20 21 22 23 24

lower jaw incisor

weak

regional

weak expression: see section 08 09 10 12 16 19 20

lower jaw molar

weak

regional

weak expression: see section 07 08 09 10 12 15 19 20

upper jaw incisor

weak

regional

weak expression: see section 08 09 11 12 17 20

upper jaw molar

weak

regional

weak expression: see section 07 08 09 10 12 15 19 20

axial skeleton

weak

regional

weak expression: see section 03 04 05 06 07 15 16

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4300
Entity Detected:	Mmd, monocyte to macrophage differentiation-associated ( MGI:1914718)
Sequence:	sense strand is shown >T4300 CGGGAGGCGCCGGGGCCGTTTAAGCGGCCTCCCTCCCGCCCCCAGGCGCCTGCTCTGGCCCGGGCCCTGT GCTGACCGACCCCCGGTGTGGCCCACTCCGGCCCTGCCCAGTTGCGTGGCTCCCGCCTGGCACGCCAGCG GCCTCGGAGCAGCTCAAGCCCATGAGGCCGGCGCGCCCTGCCGCCGGTGCAAAAGAGACGGAGCTCCCGG CCCCCGCGGTGGAGCGGTGGATCAATGCGGTTCAGGAATCGATTCCAGCGTTTCATGAACCATCGGGCCC CAGCTAATGGCCGCTACAAACCAACGTGCTACGAACATGCTGCCAATTGCTACACACACGCATTCCTCAT TGTTCCGGCCATTGTGGGCAGTGCCCTCCTCCATCGGCTGTCTGATGACTGCTGGGAGAAGATAACAGCA TGGATCTACGGGATGGGCCTTTGCGCCCTCTTCATAGTCTCCACAGTGTTTCACATAGTATCATGGAAGA AGAGCCACTTGAGAACAGTGGAGCATTGTTTCCACATGTGCGATCGGATGGTCATCTACTTCTTCATTGC TGCTTCCTACGCCCCATGGTTAAATCTCCGTGAACTTGGACCCCTGGCATCTCATATGCGTTGGTTTATC TGGCTCATGGCAGCTGGAGGAACCATTTATGTATTTCTCTACCATGAAAAGTATAAAGTGGTTGAACTTT TCTTCTATCTCACGATGGGATTTTCTCCAGCCTTGGTGGTGACATCAATGAATAACACTGACGGACTTCA GGAGCTCGCCTGTGGGGGATTGATTTACTGCTTGGGAGTTGTGTTCTTCAAGAGCGATGGCATCATTCCG TTCGCCCATGCCATCTGGCACCTGTTTGTGGCCACGGCAGCTGCCGTACACTACTATGCCATTTGGAAAT ACCTGTACCGAAGTCCCACAGACTTTATTCGGCATTTATGACCAGTCTGTGCTAGGTTTTCAAATCAGTA TTATCACGATGGAGGCACTTGGGTGGGGGGAGGGGCGGGGGGAGAACTGAATGTCGCACACAGAAGAAGA GTGAGCAAACTTGCACTGACTTCGTTTTAATTTTTTTTTAATATAATTACTGTGAAAGTATAAAAGCTGT GTCTCGGAGTTTTCCTCTTCACAGGCAACAGATGAGGTAGTGAATCAATTCTTCATTCCATTCCACTATC ATGAAGGACTCGGGAGAGACTTGGCCAACTGATGTTTACAAACCAGAAATTTTTGTATTTTTCTTTTACA GATTTTAACTACGATGATTTTTTTCTAAATTAAGTAGGTCAGGTTGTCAAAGTCAGTGCAATAGTAATAC AACTTCCTTTTTAAGAAAACCCAAAGTTGTTTCTATCACGTTTCCCGTTCACTGTGTAAAAGAATCATGG AAACTGAACACTACTTTTTACCATGTTTCATCTTGACATAACATGGTTATTTTTTAAAAAGGAGGCTTTA GTTCTTTTGTAAATTTTTAAAGAAACGATTCCATTGACGCACACCCCCACTGGCTCCTCATCCATGCGAG GTTTTTGCAGCAAACGGTCAACATTCCACGAACAAACATTATACCTCTTCTGGCAGTTTTATTAAGTGTG GAGAACTTGCCAATTTTTTAGAAACACTGCAGTTTTTTCCAACCTTTTCTGCCAATCTCTAACCCTGAAC TCAGTGCTGCTTTGGGCTGACATCCATGACTGGTGTGGGCCACCAGGAGTAAGACAGTAGTGTTTTGATA CCATCCACTTTTTCAGAAGACATAACTTCGCTACATGCCTTTGCCCCAGTTTTTCTCCAGGGTCTCTCTC CACAGTCGGGGAGGATCCTGTCCGGATTATGATAGTATTATTGGGTTGCCCATCTGCCATGAGCTTTCTG AATCATGGCGAATTGTATTTGCCACTTTGGTGCCCAACGGTTCAAGTCATTGTGGTTAAGCCACTTAACT TTATTGTATGAGTTTGATATTCATTTTAATTGTGGGACTAGACACATAAACTCACATTTCTGCCTTTTCC TCTGCATTTCTCAATATATTATGTTTTTCTCACGCCATAAATAAAACATTGATTGGCAGGTTGAAGCTTG TGTCAGTTGTTGATGGGTTTTATGCTGACTCTGTGGAGTGATATTTATATATTATTTTTAGTTTCCTCTC AATGTCTTATATTAAGATTGATGTATTAGTTGCTTTGTTGGTTAATCTTTCTTGGTGTTTAAGAGAAATG AAGTCACCTTGCCTTTAGATCAGATGCTATTATAACCTGTCTGTCTAGGAACAAGCTGTCAGACTAAAGA TATGGTTTATTCATTGAAATTCTGGACAGCTGTACGTTTAAAAGAAAGTTTATTTTGTATGTGTGTCTAG GCTCTGATTTTTGGTAAATTGTAAAGGAACAACTGAAGAACTGGTTGCTGGGGATGTCTTCAGTAGTCAG GAGTACCCAAGGATAACACCAGCCACACCGCTGTATATGCATGATTCTGAACAGTTAGCCGCCTGTTATT TTACTGTGTATATTTGACTTTAAATATATTAACTTTTTGTGGATTCATTTAAAGTCTGCTCATATGCAGC ACTGTCAAAACCACTAAACTGTATTTGAGAACTTGTGCTGTAGGTTAGAATAAAGTTGTTACTTGGGTTG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506710 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506710 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11113 same embryo
	EMAGE:11114 same embryo
	EMAGE:11112 same embryo
	EMAGE:11116 same embryo
	EMAGE:11115 same embryo
	EurExpress:euxassay_001135 same experiment
	MGI:4826399 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source