EMAGE mouse gene expression spatial database

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EMAGE:11124

Mgst1 microsomal glutathione S-transferase 1 ( MGI:1913850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001008. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001008_01	euxassay_001008_02	euxassay_001008_03	euxassay_001008_04

euxassay_001008_05	euxassay_001008_06	euxassay_001008_07	euxassay_001008_08	euxassay_001008_09

euxassay_001008_10	euxassay_001008_11	euxassay_001008_12	euxassay_001008_13	euxassay_001008_14

euxassay_001008_15	euxassay_001008_16	euxassay_001008_17	euxassay_001008_18	euxassay_001008_19

euxassay_001008_20	euxassay_001008_21	euxassay_001008_22	euxassay_001008_23	euxassay_001008_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11124_wholemount_strong.wlz

11124_wholemount_moderate.wlz

11124_wholemount_weak.wlz

11124_wholemount_possible.wlz

11124_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11124_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 24

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 20 21 22 23 24

adrenal gland

moderate

homogeneous

moderate expression: see section 09 10 11 19 20 21

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21

foregut-midgut junction

moderate

regional

moderate expression: see section 14 15 16 17 18

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 17 18 19 20

pituitary gland

weak

regional

weak expression: see section 11 12 13 14 15 16 17

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

vibrissa

weak

regional

weak expression: see section 07 08 23 24

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 12 13 14 16 17 18 19 weak expression: see section 20

telencephalon ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20 21 22 23

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16

rest of cerebellum ventricular layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

pons ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16 17

nasal capsule

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 not examined expression: see section 10 11 12 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 18 19 20 21

anal canal

moderate

regional

moderate expression: see section 16 17

esophagus

moderate

regional

moderate expression: see section 12 13 14 15

stomach

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12

hindgut

moderate

regional

moderate expression: see section 14 15 16 17 18

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 09 10 21 22 23

kidney pelvis

moderate

regional

moderate expression: see section 11 12 19 20

left lung

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23 24

right lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

trachea

moderate

regional

moderate expression: see section 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1086
Entity Detected:	Mgst1, microsomal glutathione S-transferase 1 ( MGI:1913850)
Sequence:	sense strand is shown >T1086 NAGNCTGTTGGCCTACTGGAGGACAACTTGCAGCCCTTCTCCCTGGATTCAGTCATTTGAAGACTGAGAC CAAGATTGGAAGCATGGCCGACCTCAGGCAGCTCATGGACAATGAGGTGTTGATGGCCTTTACCTCCTAT GCAACGATCATTCTTACCAAGATGATGTTCATGAGCTCTGCGACCGCATTCCAGAGGATAACCAACAAGG TTTTTGCCAACCCAGAAGACTGCGCTGGCTTTGGCAAGGGAGAGAATGCCAAGAAGTTTGTTCGCACTGA CGAGAAGGTGGAACGCGTGCGCAGAGCCCACCTGAATGATCTTGAAAACATCGTTCCCTTTCTCGGCATC GGCCTCCTGTACTCCCTGAGTGGACCAGATCTCTCTACAGCCCTCATGCACTTCAGAATCTTTGTAGGTG CTCGGATCTACCACACCATTGCTTACTTGACTCCCCTTCCTCAGCCAAACAGGGGCTTGGCATTTTTTGT TGGCTATGGAGTTACTTTGTCAATGGCTTACAGGCTGCTAAGGAGCAGACTGTACTTGTAAAGA
Notes:	The probe template was PCR amplified from IMAGE:2123093 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2123093 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11126 same embryo
	EMAGE:11123 same embryo
	EMAGE:11125 same embryo
	EMAGE:11122 same embryo
	EMAGE:11127 same embryo
	EurExpress:euxassay_001008 same experiment
	MGI:4826190 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source