EMAGE mouse gene expression spatial database

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EMAGE:11157

Pla2g4a phospholipase A2, group IVA (cytosolic, calcium-dependent) ( MGI:1195256)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003564. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003564_01	euxassay_003564_02	euxassay_003564_03	euxassay_003564_04

euxassay_003564_05	euxassay_003564_06	euxassay_003564_07	euxassay_003564_08	euxassay_003564_09

euxassay_003564_10	euxassay_003564_11	euxassay_003564_12	euxassay_003564_13	euxassay_003564_14

euxassay_003564_15	euxassay_003564_16	euxassay_003564_17	euxassay_003564_18	euxassay_003564_19

euxassay_003564_20	euxassay_003564_21	euxassay_003564_22	euxassay_003564_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11157_wholemount_strong.wlz

11157_wholemount_moderate.wlz

11157_wholemount_weak.wlz

11157_wholemount_possible.wlz

11157_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11157_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 19 20 21 22 23

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 02 weak expression: see section 03 04 05 06 07 08 19 20 21 22 23

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 06 18

4th ventricle lateral recess

strong

regional

strong expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 11

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 21 22 23

nasal septum

moderate

regional

moderate expression: see section 10 11 12 13 14 15

viscerocranium

moderate

regional

Expression in the turbinate bone.

hindgut

strong

regional

strong expression: see section 13 14 15 16

rectum

strong

regional

strong expression: see section 14

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

bladder

strong

regional

Expression in the fundus region.

kidney calyx

strong

regional

strong expression: see section 07 08 10 11 17 18 20 21

kidney pelvis

strong

regional

strong expression: see section 09 19

urethra of male

moderate

regional

moderate expression: see section 14 15

thyroid cartilage

moderate

regional

moderate expression: see section 12 13 14 15

trachea

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5978
Entity Detected:	Pla2g4a, phospholipase A2, group IVA (cytosolic, calcium-dependent) ( MGI:1195256)
Sequence:	sense strand is shown >T5978 GAAGACTTTCTAGAAGTCGGAACTGTGAAGGGCTCCCGACTGAGAGCCAGAAGGCACAGAGAAGCCTGAG GATTCTCATTTAACTCTGGGAACTGCTTCAAGAAGCTACAGTACCATAGAAGACCTGGGAAGTGTGAGAA TTTCTGCAACTGGGACCAAAATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTC CCATAAGTTTACTGTTGTGGTTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGAC ACTCCAGATCCTTATGTGGAACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCA ATAATGATATAAACCCCGTGTGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTT GGAGATCACACTGATGGATGCCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCT TCAATGAAAGTGGGAGAGAAGAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAA TGTCTCTGGAAGTTTGTTCATGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTT CAGACAGCAGAGGAAAGAGAACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGG CTTTATTCCACACGTGATGTGCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGG GATTCTCTGGTGTGATGAAGGCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCT TTCTGGATCCACATGGTACATGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAG ATTAATGAAGAGCTAATGAAAAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAA GATACGTTGAGTCTTTATGGAAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGAT GCTAATAGGAGAAACACTAATTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAAT GCCGCCCGGTGTCCTTTGCCTCTCTTCACGTGTCTCCACGTCAAACCTGATGTGTCAGAGCTGATGTTTG CCGATTGGGTGGAATTTAGTCCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCT ATTTGGAAGCAAGTTTTTTATGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATG GGTGTCTGGGGCAGTGCCTTTTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAG GCTCTACAATGGAAGAGGAATTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAG TGATGATGAGGCTCAAGGACCCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAAC CAAGCAAGTTGGGTCCATCGGATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAG GACGTGCCGGAAAGGTGCATAACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCT GAGAGACTTCAGCTCTCAGGATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTT GAACGAATATATGAACCACTGGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTA ACCTGCCGTATCCCTTGATTCTGCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGC AAGGCCGAGTGACACCAGTCCCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAG CTTCCCTTTCCAAAGATCGATCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAAC CTAAGAATCCTGATGTGGAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAG AAAGTACAAGGCCCCAGGTGTTCTAAGGGAAACCAAAGAAGAGAAAGAAATCGCTGACTTTGACATTTTT GATGACCCCGAATCGCCATTTTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTGCATG ATCTGATGTACTTCAACACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATA CAGAAGACAGAACCCATCTCGTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAG GAGTTTCTAAGTAAACCCACTGTGTAATTTCTGTGCTGGGATGATCAAGCCATTTGAATTCCATGACAAT TTGAGTTCAGAACACATTAGAGGTCATCTTACTATGCAGAAGAGACTGGCTGCTACTCAGAGTTGTGGAG ATTTAGCCATGTGTTAGGTGAAAATGATGTTGATTATGTAATACTTAGCAACAGTTTCTGACAGTATGAA TTTTTTGACATTAGCATAGAGCTATATACTGTATTTTAAACATTCCTCACATTTTTTACCTGTACTTTTT ATATAAATATGACATGTCTTTTCTTTTGAAAATATTTAATAGTTTAACTCAGTAAAAGAGACTTCCCATT GTGTGTGAATGTTATTCCGAACTAGATTTGTTCATGCCATGTTACAACACTATTTTTATTTAAATGTTTA TATTTACACATACGAAATAAATACTTTGCTGTACAAATTACCACTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485483 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485483 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11153 same embryo
	EMAGE:11152 same embryo
	EMAGE:11155 same embryo
	EMAGE:11154 same embryo
	EMAGE:11156 same embryo
	EurExpress:euxassay_003564 same experiment
	MGI:4827241 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source