EMAGE mouse gene expression spatial database

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EMAGE:11160

Kitl kit ligand ( MGI:96974)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003584. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003584_01	euxassay_003584_02	euxassay_003584_03	euxassay_003584_04

euxassay_003584_05	euxassay_003584_06	euxassay_003584_07	euxassay_003584_08	euxassay_003584_09

euxassay_003584_10	euxassay_003584_11	euxassay_003584_12	euxassay_003584_13	euxassay_003584_14

euxassay_003584_15	euxassay_003584_16	euxassay_003584_17	euxassay_003584_18	euxassay_003584_19

euxassay_003584_20	euxassay_003584_21	euxassay_003584_22	euxassay_003584_23	euxassay_003584_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11160_wholemount_strong.wlz

11160_wholemount_moderate.wlz

11160_wholemount_weak.wlz

11160_wholemount_possible.wlz

11160_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11160_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

vibrissa

strong

regional

strong expression: see section 07 08 09 20 21 22 23

thalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 15 16 17 18 19

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 12 13 14 20 21 22 23 24

olfactory cortex

strong

regional

strong expression: see section 10 11 12 13 16 17 18 19

telencephalon ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 10 11 13 16 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 08 19 20 21 22

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 19 20 21 22 23

pons mantle layer

strong

regional

strong expression: see section 20

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 10 13 18 19 20

ventral grey horn

strong

regional

strong expression: see section 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 09 10 11 19

not examined

homogeneous

not examined expression: see section 02 03 04 24

neural retina

strong

regional

strong expression: see section 02 03 04 24 not examined expression: see section 23

naris

strong

regional

strong expression: see section 12 13 14 15 16 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17 18 19 20

maturing glomerular tuft

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21

trachea

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6715
Entity Detected:	Kitl, kit ligand ( MGI:96974)
Sequence:	sense strand is shown >T6715 CGAGAGTGCCGCGGGAAAGCAACGGCCAAGGACGGGGCGCTGCGTTCGAGCTACCCAATGCTGGGACTAT CTGCAGCCGCTGCTGGTGCAATATGCTGGAGCTCCAGAACAGCTAAACGGAGTCGCCACACCGCTGCCTG GGCTGGATCGCAGCGCTGCCTTTCCTTATGAAGAAGACACAAACTTGGATTATCACTTGCATTTATCTCC AACTGCTCCTATTTAATCCTCTCGTCAAAACCAAGGAGATCTGCGGGAATCCTGTGACTGATAATGTAAA AGACATTACAAAACTGGTGGCAAATCTTCCAAATGACTATATGATAACCCTCAACTATGTCGCCGGGATG GATGTTTTGCCTAGTCATTGTTGGCTACGAGATATGGTAATACAATTATCACTCAGCTTGACTACTCTTC TGGACAAGTTCTCAAATATTTCTGAAGGCTTGAGTAATTACTCCATCATAGACAAACTTGGGAAAATAGT GGATGACCTCGTGTTATGCATGGAAGAAAACGCACCGAAGAATATAAAAGAATCTCCGAAGAGGCCAGAA ACTAGATCCTTTACTCCTGAAGAATTCTTTAGTATTTTCAATAGATCCATTGATGCCTTTAAGGACTTTA TGGTGGCATCTGACACTAGTGACTGTGTGCTCTCTTCAACATTAGGTCCCGAGAAAGATTCCAGAGTCAG TGTCACAAAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGTAAT AGGAAAGCTGCAAAGTCCCCTGAAGACTCGGGCCTACAATGGACAGCCATGGCATTGCCGGCTCTCATTT CGCTTGTAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAAACAGTCAAGTCTTACAAGGGCAGT TGAAAATATACAGATTAATGAAGAGGATAATGAGATAAGTATGTTGCAACAGAAAGAGAGAGAATTTCAA GAGGTGTAATTGTGGACGTATCAACATTGTTACCTTCGCACAGTGGCTGGTAACAGTTCATGTTTGCTTC ATAAATGAAGCAGCCTTAAACAAATTCCCATTCTGTCTCAAGTGACAGACCTCACCCTTACCTGTTCTTG CTACCCGTGACCTTGTGTGGATGATTCAGATGTTGGAGCAGAGTGCTTCGCTGTGAACCCTGCACTGAAT TATCATCTGTAAAGAAAAATCTGCACGGAGCAGGACTCTGGAGGTTTTGCAAGTGATGATAGGGACAAGA ACATGTGTCCAGTCTACTTGCACCGTTTGCATGGCTTGGGAAATGTCTGAGTGCTGAAAACCCACCCAGC TTTGTTCTTCAGTCACAACCTGCAGCCTGTCGTTAATTATGGTCTCTGCAAGTAGATTTCAGCCTGGATG GTGGGGGGAATTTTTTTTTCACAAAGGGATGTAGAAAACAATTTTAAAAAAACAAAACAAAACAATAACT CCGTAGCATCAGCCACTGTATGGAGTTGAAAGCCATGGGGAGTATTTTTTTTTTTCTTTTGAGCAGGCTC ATCCGCTCTGTAGTTAATGATTAAGGAGAGCAGCCTAAATGTAAGCTCCTCCTGACAGAAGTCATGGTGC ACCGTATCCTACTAGAGAAATGGACATGGGTAGCTAGTTCTATCCATGCGGTGGAGCCCATGCTGAACTG CAGCATAATACCACGGCATATGGAAGACTCTCCAGAAAAGTCCTTACAGGAGAAAATTGTCAACGTGGAC CAGTGGAAGAACATGCCAAGGAAGACATTTGGCCCATCGTGAAGCCAGTCTTTTCCCTTGACAGTCACTC TAGCGTGTGTAAATCGTGTTTGCAATAGTATAATGTGACATGCTTATGTTTTGAAACTACTGCAGCGGTA TGAAGACAGATATGGGAGGAATGCTGAATGTGCAAGGGTCTCCCCACCCCCACCCCCCTTTTATTTTAAA TCCTGATGGGAGAGAATAAGAACGGATGATGGTATTTAGTACCTAAGATTAGATGCGAGTACTTTATTCT AATACGGGAGTTGCAAACTAAGTCACTCATATTCTAAAAGAATGCAAGGACTCGGAAAACATTAGTTATT TGCAGTATAACCGTTATCTCTTGAAACATAGCCACTTATTTAAATCGGGCACTCTGTATTCATTAAGGTT TATGAAACGAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3662792 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3662792 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11163 same embryo
	EMAGE:11161 same embryo
	EMAGE:11158 same embryo
	EMAGE:11162 same embryo
	EMAGE:11159 same embryo
	EurExpress:euxassay_003584 same experiment
	MGI:4825779 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source