EMAGE mouse gene expression spatial database

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EMAGE:11238

Onecut1 one cut domain, family member 1 ( MGI:1196423)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003197. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003197_01	euxassay_003197_02	euxassay_003197_03	euxassay_003197_04

euxassay_003197_05	euxassay_003197_06	euxassay_003197_07	euxassay_003197_08	euxassay_003197_09

euxassay_003197_10	euxassay_003197_11	euxassay_003197_12	euxassay_003197_13	euxassay_003197_14

euxassay_003197_15	euxassay_003197_16	euxassay_003197_17	euxassay_003197_18	euxassay_003197_19

euxassay_003197_20	euxassay_003197_21	euxassay_003197_22	euxassay_003197_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11238_wholemount_strong.wlz

11238_wholemount_moderate.wlz

11238_wholemount_weak.wlz

11238_wholemount_possible.wlz

11238_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11238_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 14 15 16

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

diencephalon lateral wall marginal layer

strong

regional

strong expression: see section 11 12

telencephalon mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 07 08 09 10 15 16 17

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17

metencephalon rest of alar plate mantle layer

strong

regional

strong expression: see section 16

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 14 15 17 18 19

pons ventricular layer

strong

regional

strong expression: see section 11 13

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18

midbrain marginal layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 moderate expression: see section 07 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 20 21 22 moderate expression: see section 07 08 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 moderate expression: see section 19

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 09 10 16 17 moderate expression: see section 11 15 weak expression: see section 18

not examined

homogeneous

not examined expression: see section 01 02 03 22 23

neural retina

strong

regional

strong expression: see section 01 02 03 04 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4904
Entity Detected:	Onecut1, one cut domain, family member 1 ( MGI:1196423)
Sequence:	sense strand is shown >T4904 GCTCGAGCTGGCGGGCGGCACAGGCAGGCAGCAGCCGCGGCAGGCGCACGGGCCGCGTCAAGGGAGCCTG GGGCAGCAGGATTCTAAGAAGAGGGGCGAGAGGGGGCCGGGCTGGGTGGGCTAGGGGTACCGCGCTCTCC CAACAGCACGGGTCCTTTTTGGAAATTAATATTAAAAGAAAAAAAAAAGACAGAGGGGAAGGTGGGAGCA AGAGAGAAGGCAAGACACACACAGAGAGAGCGAGAGACACAGATCCCCACAGTGAGAGGAAGAAAGGCCA CAGTCGCAGGCAGCCGATGTGAAGACTGGACTCCTTGCGCCCCTCGCCGCCTCTGCCCGGCCACATCGAT GTTGCAGCCCGCTCGCCCGCATCACGATGAACGCACAGCTGACCATGGAGGCGATCGGCGAGCTGCACGG GGTGAGCCATGAGCCGGTGCCCGCCCCTGCTGACCTGCTGGGCGGCAGCCCTCACGCGCGCAGCTCCGTG GGACACCGCGGCAGCCACCTGCCTCCCGCGCACCCGCGTTCCATGGGCATGGCGTCCCTGCTGGACGGCG GCAGCGGAGGCAGCGATTACCACCACCACCACCGCGCCCCTGAGCACAGCTTGGCTGGCCCCCTGCACCC CACCATGACCATGGCCTGTGAAACTCCCCCAGGTATGAGCATGCCCACCACCTACACTACCTTAACCCCT CTGCAGCCGCTGCCGCCCATCTCCACCGTGTCCGACAAGTTCCCTCACCATCATCACCACCACCATCACC ACCACCACCCACACCACCACCAGCGCCTGGCGGGCAACGTGAGCGGTAGTTTCACACTTATGCGGGATGA GCGCGGGCTGGCCTCTATGAATAACCTCTATACCCCCTACCACAAGGACGTGGCTGGCATGGGCCAGAGC CTCTCGCCCCTCTCTGGCTCCGGTCTGGGCAGCATTCACAACTCCCAGCAAGGACTTCCCCACTATGCTC ATCCCGGCGCGGCTATGCCCACCGACAAGATGCTCACCCCAAATGGCTTTGAAGCCCACCACCCTGCCAT GCTCGGTCGCCACGGGGAGCAGCACCTCACGCCCACCTCGGCCGGCATGGTACCCATCAACGGCCTTCCT CCGCACCATCCTCATGCCCACCTGAATGCCCAGGGCCACGGACAGCTCCTGGGCACAGCCCGAGAGCCCA ACCCTTCGGTGACCGGCGCGCAGGTCAGCAATGGAAGTAATTCAGGGCAGATGGAAGAGATCAATACCAA AGAGGTGGCGCAGCGTATCACCACCGAGCTCAAACGTTACAGCATCCCACAGGCCATCTTCGCGCAGAGG GTGCTCTGCCGTTCCCAGGGGACCCTTTCGGACCTGCTGCGAAACCCCAAGCCCTGGAGCAAACTCAAGT CGGGTCGGGAGACCTTCCGGAGGATGTGGAAGTGGCTGCAGGAGCCGGAGTTCCAGCGCATGTCGGCGCT CCGCTTAGCAGCCTGCAAACGGAAAGAGCAAGAACATGGGAAGGACAGAGGCAACACCCCCAAAAAGCCC AGGCTGGTCTTCACAGACGTCCAACGTCGAACTCTACATGCAATATTCAAGGAAAATAAGCGTCCGTCCA AAGAATTACAAATCACCATCTCCCAGCAGCTGGGGTTGGAGCTGAGCACTGTCAGCAACTTCTTCATGAA TGCCAGGAGGAGGAGTCTGGACAAGTGGCAGGACGAGGGCGGCTCCAACTCAGGCAGTTCATCGTCCTCA TCGAGCACTTGTACCAAAGCATGAGGGAGGAAGAACCACGGACTAGTACCTCGGTGGAAAAGCTTTAAAT TAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5101697 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5101697 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11239 same embryo
	EMAGE:11236 same embryo
	EMAGE:11237 same embryo
	EMAGE:11235 same embryo
	EMAGE:11234 same embryo
	EurExpress:euxassay_003197 same experiment
	MGI:4826972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source