EMAGE mouse gene expression spatial database

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EMAGE:11281

Pitx1 paired-like homeodomain transcription factor 1 ( MGI:107374)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006721. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006721_01	euxassay_006721_02	euxassay_006721_03	euxassay_006721_04

euxassay_006721_05	euxassay_006721_06	euxassay_006721_07	euxassay_006721_08	euxassay_006721_09

euxassay_006721_10	euxassay_006721_11	euxassay_006721_12	euxassay_006721_13	euxassay_006721_14

euxassay_006721_15	euxassay_006721_16	euxassay_006721_17	euxassay_006721_18	euxassay_006721_19

euxassay_006721_20	euxassay_006721_21	euxassay_006721_22	euxassay_006721_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11281_wholemount_strong.wlz

11281_wholemount_moderate.wlz

11281_wholemount_weak.wlz

11281_wholemount_possible.wlz

11281_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11281_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot

moderate

regional

moderate expression: see section 04 07

leg

moderate

regional

moderate expression: see section 01

lower leg mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06

upper leg mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 21 22 23

anterior abdominal wall muscle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 weak expression: see section 01 02 03 04 05 06 07 08 09 10 20 21 22 23

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

adenohypophysis

strong

regional

strong expression: see section 11 12 13 14 15 moderate expression: see section 10

vibrissa

weak

regional

weak expression: see section 05 06 07

midbrain mantle layer

strong

single cell

strong expression: see section 04 05 06 07 08 15 16 17 18

eye

moderate

regional

moderate expression: see section 03 04 05 23

cornea

moderate

regional

moderate expression: see section 02

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 15 moderate expression: see section 11 12 13 16 17 18 19 20 21 weak expression: see section 08 09 10

esophagus epithelium

strong

regional

strong expression: see section 09 10 11 12 13

pharynx epithelium

strong

regional

strong expression: see section 10 11 14 15 16

tongue epithelium

strong

regional

strong expression: see section 15 moderate expression: see section 11 12 13 14 16

frenulum

strong

regional

strong expression: see section 15 moderate expression: see section 12 13

tongue muscle

moderate

regional

moderate expression: see section 11 12 15 16

stomach

strong

regional

strong expression: see section 03 04 05 06 07 moderate expression: see section 08 09 10 11 12

midgut

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 14 15 16 17 18 19

lower lip

moderate

regional

moderate expression: see section 06 07 08 09 10 18 19 20 21 22

mandible

strong

regional

strong expression: see section 03 moderate expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 12 16

lower jaw molar

moderate

regional

moderate expression: see section 06 07 20

oral epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 17

upper jaw molar

moderate

regional

moderate expression: see section 06 07 20

bladder

moderate

regional

moderate expression: see section 13 14 15 16 17

genital tubercle of male

moderate

regional

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7368
Entity Detected:	Pitx1, paired-like homeodomain transcription factor 1 ( MGI:107374)
Sequence:	sense strand is shown >T7368 CCACGCGTCCGCCCGAGCCTTAGAAACCGGCCTCGAATCGGGACTGGGGCCCGGGCCGGCCGGGCAGCTG AGGCCGGAGCAGCAGGAGCGCGTGGCGGAGCCCCGAGCCCCGAACCCGGTCCCGCAGCCTGGAGAGGTCG ATGGAGCTCGAACGGCGTCGCTGTGGGCGCGGGCAGCCCTGGGGGGCAGCCGCTTAGGCGCCACTCTCTT GTCCCCAACAGGTCGCAGCCTGGGCGGCCGGGAGCTCCCAGCCCCGGCCCCTGGAGCGCCCGTTGCGGTC CCCACCTCCATGGACGCCTTCAAGGGAGGCATGAGCCTGGAGAGGCTACCGGAGGGGCTGCGGCCACCGC CACCACCACCGCACGACATGGGGCCCAGCTTCCACCTGGCCCGCGCCGCCGACCCCCGGGAGCCGCTGGA GAACTCCGCCAGCGAATCGTCCGACGCTGATCTGCCAGACAAGGAGCGCGGTGGGGAAGCCAAGGGGCCA GAGGATGGTGGCGCGGGCAGTGCTGGCTGCGGCGGCGGTGCAGAGGACCCAGCTAAGAAGAAGAAACAGC GGCGGCAACGCACTCACTTCACAAGCCAGCAGTTGCAAGAGCTGGAGGCCACGTTCCAAAGGAACCGCTA CCCCGACATGAGCATGAGAGAGGAGATCGCGGTGTGGACCAACCTCACTGAACCGCGAGTGCGGGTCTGG TTCAAGAACCGGCGAGCCAAATGGCGCAAGCGGGAGCGGAACCAGCAGTTGGACCTGTGCAAGGGCGGCT ATGTGCCGCAGTTCAGCGGCCTGGTGCAGCCCTACGAGGACGTGTACGCTGCCGGCTACTCCTACAACAA CTGGGCGGCCAAGAGCCTGGCCCCGGCGCCGCTGTCTACCAAGAGCTTTACCTTCTTCAACTCCATGAGC CCGCTCTCCTCTCAATCCATGTTCTCGGCCCCCAGCTCCATCTCTTCCATGACCATGCCGTCCAGCATGG GCCCGGGCGCCGTGCCCGGCATGCCCAACTCGGGCCTCAACAACATCAACAACCTCACCGGCTCCTCGCT CAACTCGGCCATGTCGCCGGGCGCCTGCCCCTACGGCACCCCAGCCTCGCCCTACAGCGTCTACCGGGAC ACGTGCAACTCGAGCCTTGCCAGCCTGCGGCTCAAGTCCAAGCAGCACTCGTCGTTTGGCTATGGCGGTC TGCAGGGTCCGGCGTCGGGGCTCAACGCTTGCCAGTACAACAGCTGACCGCCCCGCCTGGTCCCCGGGGC CGCCGGCCAGCAAGGCTCACAGAAGGGAGCGCGCGGGAGCACGCGGGGCGCTCGCAGCTCCTCGGACCTC ACCTCTGCGCAGCCCCTTCCTCCCCACCCCGCCTCCAGGTTGGTTTTGTGTTTGCTTTTTCCAGACCCCC GCTCTGTCCCCCAATGAATGAAAAGAAAAGAAAGCAAGCCAAGGGAGGGGGTCGGGTGGGGAAGAAAGAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4192818 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4192818 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11282 same embryo
	EMAGE:11283 same embryo
	EMAGE:11284 same embryo
	EurExpress:euxassay_006721 same experiment
	MGI:4827224 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source