EMAGE mouse gene expression spatial database

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EMAGE:11303

Zcchc5 zinc finger, CCHC domain containing 5 ( MGI:2685221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006560. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006560_01	euxassay_006560_02	euxassay_006560_03	euxassay_006560_04

euxassay_006560_05	euxassay_006560_06	euxassay_006560_07	euxassay_006560_08	euxassay_006560_09

euxassay_006560_10	euxassay_006560_11	euxassay_006560_12	euxassay_006560_13	euxassay_006560_14

euxassay_006560_15	euxassay_006560_16	euxassay_006560_17	euxassay_006560_18	euxassay_006560_19

euxassay_006560_20	euxassay_006560_21	euxassay_006560_22	euxassay_006560_23	euxassay_006560_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11303_wholemount_strong.wlz

11303_wholemount_moderate.wlz

11303_wholemount_weak.wlz

11303_wholemount_possible.wlz

11303_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11303_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03

upper leg muscle

strong

regional

strong expression: see section 03 04 05 06 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 02

shoulder joint primordium

strong

regional

strong expression: see section 02

shoulder rest of mesenchyme

strong

regional

strong expression: see section 01

hand

strong

regional

strong expression: see section 01 02 04 05

hand mesenchyme

strong

regional

strong expression: see section 03

foot

strong

regional

strong expression: see section 04 05 06 07 08 09 22 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue extrinsic muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

tongue intrinsic muscle

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19

tail paraxial mesenchyme

strong

regional

strong expression: see section 09 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7953
Entity Detected:	Zcchc5, zinc finger, CCHC domain containing 5 ( MGI:2685221)
Sequence:	sense strand is shown >T7953 CAAAATAGCTACGTTACAGAGGAGAGTTGGAACGTGTTCTGGGAGTGGGTGTGTTTGTTTGAGTTCAGTG TGTGCAGAAAAGGCAAGCCTCCGCAGCCATTCACACACTTCTCTCCTCAGCGATGGCTATGCAGACAGCA GAGCTCCAGCCCTACATCTCTAAATGTGTGTCTCACACTTCCCGGTGCTGAGCTCTAAGGCTCCCAGTGA CCTCTTGATAATCAGCCTTGGCTGGGTCCAAATCCCCACCCCTTGGAAAAGTCACGGAGCCTGACGTCTG TCTGCCTGTGAGCCGCTTTCAGCAAAGACCTCACAAGCAGCTACTCAATCTCTCTTGGTAGCAAGTTGCC AAACCAGGCCCGTTTCCTCAAAAGATGGTAGAGGACTTAGCAGCTTCCTATGTTACTCTGAAATTGGAGA ATGAAATTCTGCAGGCTCAAGTGAAAAGGCTCATGGAAGAAAATGCTGCCCTCCAGGCCCAGATACCAGA GCTCCAGAAATCCGGAGCAGTCAAAGAGCATGAACCACTCCGAAAGCCCTCAGAGGCCCAAGAGCCCCCA GAGTCCCCAGAGTTCCCAGCAGCCAGGGAGTCACAGAACCCCTGGGAACCCCCAGCAACCACAGAGCCCG GGGAGCCCACAAAGATCAGAGAGCCCAGGGAACCCTCAGCCATCAGTGAGCTCAGGGAACCCCCAGAGAT CAAGGAGCCCCAGGAGCCCCCAGAGACCAATGAGTCTGGAGAATCCTCAGCCATCACAGAGTTCAGGGGA TCCCCAGAGATCAAGGAGCCCCATTTGCCCCCAAAGTCCAAGGAGTACTGGGGACCTCAGGAGCTTCCAA TGGTCAAGGAATCCTGGGAGCCCCCAGAGGCCCTGGATTCCACAGCCTGGAAGCCACCAGCAGTCAAGGA ATCCCAGGAGATCCAGAAGGCTCTGGAGTCCCCAGCAACCCAGAGGCCCCAGGCGTCCCCAAGGGGTTAT GATCTCCCAGCAGTCTGTGAGGCGGAGCCCACAGACAACCAGGGTACCCCAGCCCTCAAGGAGCTTCAGA GTCCACAGCTCCACAACCCTACAAATGATGAGGAGTCTCAGACGGTTCCAGAATACCAGGAGACCTCATC ACAGCTGGAGCCCCTTGAGCATCCACCTCCCCAGGAGACTCTGGAGCCTCGGGTGCCCCAGGAGCCCCTG GACCCCTCAGATGCCGAGGAGTTTCTAGAACTCTCAGTACCTGAAGAGTCCCTAGAGGGCCTGATAGTTG CGAGAACAGGAACAGAACAGGCCCAGTGTGAATTAGAGGCTACAACTTTGCCCCTAGAGTACCCTTTGGC CTTCAGTGAGGATTTTCAGAAACTCTCTGAGTTTTTAGTTCAGTTGACCAGTTACCTGAGAAGCAGAGGG TATCCCACTGAAGCGGCTCTAGTGAGCTTTGTTGGCAGCTTCTTCTCAGGTGAGGCGGGAAGGATGTTCC AGCCCTTATTAGATAGCCAACCCCCCCTGGTGGAGCAATTTGAAAGGCTCCTGCGAGCGCTCCAAGATAC TTTTGACAATCCAGAAAGCTTGGAAGTTGCTAACCAGGGCCTCCCTCAACTTCGCCAAGGGGAAGGCCTT GCTCCCAGGTACTCAACCCGCTTCCATCTCATTGCGCAAGAGTTAGATTTGGGTGAAAGCACGCTCTGCA TCCAATTTCAAGAAGAGCTTGCCAGTTCTATTCAAAATGAACTGTCTTGCACAAGTCCAGCCACCAACCT ATCTGATGTGATCATTGAGTGTGTCACCCTAGAAGAGAAGGCAAGTGGCGGGGTTGATTCCAGTTCATCA TCGTCTGAAGAGGAAAATGGGTCCGAGGGTCCTCCCACTGAAAACCAACCTGTTCAAGCTACAAGTAATC GCCCCCACCTCAGCGAGGCTGAACGGGCAAGACGCCGCGAAGGCCACTTGTGCCTCTACTGTGGCCATCC AGGTCATTTTGCCAGAGACTGCCCTGTCAAGCCTCATCGTGTCCAGCAGGCGGGAAACATGGAGGCCCGG CGGTAAGGGGGATCCCGGGCGGGCCAATCTACAAATACGCGTCCCCCTCTCTTCCCATATCTATGTCCCG TACCCTGTGGCTAACTGTCGAGATTAGGCTTGGAAGACGGCAATTCTTTGAGCAAGCAATGGTGGATTCA GGCTCCTACAGAAACAGCATCGACCGCTCTGTGGTTCTTCGAGAGAAGATACCAATCCGCAATAATATCT TCCCTCTTATGCTTGAAACGGTCGATGGGCGTCCGCTGATTAATGGACCCATTACCAAAGAAACCCCACC TGTTGAAGTAAAAATTGGAAACCACGTTGAAGAGCTCCAGTTTGACATTATTCATGCACCACGCTACCCT CTGATTCTTGGAATCCATTGGCTTGAGACCCATGACCCAAACATCGAATGGAGTACCCGAACTGTGTCAT TTCCATCGCATTACTGTCATTACAATTGCTTCAGGCACAGGTGGAATCGAAGACGGCGTGATGAAATAAT TGCTGGTTAGATGGTGGGTCCTAGTGACTTCTGGCCATCTGTCTTCTGTTACCTCAGCTGTCATCAGCAT TTGCTGCTCTCTGAATCTTTCACTGAACCTCTGGCTATGAACGAAGGCTACCTGTACAACGACGCTGCCT CTTGGATGATGGACTGAACCTGACGACTTTGAGCTGCTTTTCCTCACCAACCTTGCATGCCTCCCTCTTT GAGCCCTGCATTATGTAGGGCTGTTTTTTTTTAATCGCAATTCTCACTAACGATATGCATGCGAATAGAT ATTAGAAACTAATACTGAAAACTGTAGTTGTTAGACCGATTTTGTTACTTTCTAGTCAAAATTACCACAT ATTTTCTGGTTTTTTTTTTCAATAAAAGCAATTTTCAAGTGTATTTTTCAGTAATCAATAAACAATGGGA AGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30139133 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30139133 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11305 same embryo
	EMAGE:11306 same embryo
	EMAGE:11302 same embryo
	EMAGE:11301 same embryo
	EMAGE:11304 same embryo
	EurExpress:euxassay_006560 same experiment
	MGI:4829275 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source