EMAGE mouse gene expression spatial database

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EMAGE:11323

Ramp2 receptor (calcitonin) activity modifying protein 2 ( MGI:1859650)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006585. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006585_01	euxassay_006585_02	euxassay_006585_03	euxassay_006585_04

euxassay_006585_05	euxassay_006585_06	euxassay_006585_07	euxassay_006585_08	euxassay_006585_09

euxassay_006585_10	euxassay_006585_11	euxassay_006585_12	euxassay_006585_13	euxassay_006585_14

euxassay_006585_15	euxassay_006585_16	euxassay_006585_17	euxassay_006585_18	euxassay_006585_19

euxassay_006585_20	euxassay_006585_21	euxassay_006585_22	euxassay_006585_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11323_wholemount_strong.wlz

11323_wholemount_moderate.wlz

11323_wholemount_weak.wlz

11323_wholemount_possible.wlz

11323_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11323_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 18 19 20

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22 23

hindbrain meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain meninges

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

spinal cord meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

aorta

strong

regional

strong expression: see section 10 11 12 13

heart atrium

moderate

regional

moderate expression: see section 07 13 14 15 16 17 18

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13 14

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 weak expression: see section 01

hindgut

moderate

regional

moderate expression: see section 14

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

bladder

weak

regional

weak expression: see section 11 12 13 14

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7971
Entity Detected:	Ramp2, receptor (calcitonin) activity modifying protein 2 ( MGI:1859650)
Sequence:	sense strand is shown >T7971 TCGCCATCTCACCCAAGGCGTGATGGCCCCGCTCCGGGTAGAGCGCGCCACGGGTGGATCTCGGCTTGGT GTGACCCGCGCCCAGCGGCCGACAGCGTTGTGCCTCCCTCCGCTGTTGCTGCTGCTGCTGCTGCTGCTGG GCGCTGTCTCAGCCTCTCCGGAGTCCCTGAACCAATCTCTTCCGGAGTCCCAGAATCAATCTCATCCCAC TGAGGACAGCCTTGTGTCAAAAGGGAAGATGGAAGACTACGAAACACATGTCCTACCTTGCTGGTATGAG TACAAGAGTTGCATGGACTCTGTCAAGGACTGGTGCAACTGGACTTTGATTAGCAGGCATTACAGCGACC TGCAGAACTGCTTGGAATACAATGCAGACAAGTTTGGGCTGGGCTTCCCAAATCCCTTGGCAGAAAACAT CATCCTTGAGGCTCACCTGATACACTTTGCGAACTGCTCCCTGGTGCAGCCCACCTTCTCTGATCCCCCA GAGGATGTGCTCCTGGCCATGATCATAGCCCCCATCTGCCTCATCCCGTTCCTTGTTACTCTTGTGGTGT GGAGGAGTAAAGACAGCGATGCCCAGGCCTAGGGTCCATTTCTCAGCAGCCATTTTTCCCCCCTTTTCCC TGCTGGAACCAGGAATGGCGCTCCTCCCCTCCCTACCCACTTACTCTCATCCTTCCCACAGACCTGTGGA TTGGTGGAAATGGCAGCAAAGGGGACTCACGACACAATGTTTGTAGTCTTTAAAATAAAGTTATGTTTTT GTACAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30134809 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30134809 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11320 same embryo
	EMAGE:11324 same embryo
	EMAGE:11321 same embryo
	EMAGE:11322 same embryo
	EMAGE:11319 same embryo
	EurExpress:euxassay_006585 same experiment
	MGI:4825298 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source