EMAGE mouse gene expression spatial database

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EMAGE:11324

Krt6a keratin 6A ( MGI:1100845)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006577. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006577_01	euxassay_006577_02	euxassay_006577_03	euxassay_006577_04

euxassay_006577_05	euxassay_006577_06	euxassay_006577_07	euxassay_006577_08	euxassay_006577_09

euxassay_006577_10	euxassay_006577_11	euxassay_006577_12	euxassay_006577_13	euxassay_006577_14

euxassay_006577_15	euxassay_006577_16	euxassay_006577_17	euxassay_006577_18	euxassay_006577_19

euxassay_006577_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11324_wholemount_strong.wlz

11324_wholemount_moderate.wlz

11324_wholemount_weak.wlz

11324_wholemount_possible.wlz

11324_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11324_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 07 08 09 10 11 12

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vibrissa

strong

regional

strong expression: see section 01 02 03 04 05 06 07 17 18 19 20

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 13 14 15 16 17 18 19 20

eyelid

strong

regional

strong expression: see section 01 02 19 20

anterior naris

strong

regional

strong expression: see section 09 10 11 13 14 15

external naris

strong

regional

strong expression: see section 09 10 11 15

esophagus epithelium

strong

regional

strong expression: see section 08 09 10

pharynx epithelium

strong

regional

strong expression: see section 08 09 10 11

tongue epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13

stomach

strong

regional

strong expression: see section 01

stomach proventricular region

strong

regional

strong expression: see section 02 03 04 05 06 07

lower jaw incisor

strong

regional

strong expression: see section 09 12 13

lower jaw molar

strong

regional

strong expression: see section 16

oral epithelium

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

upper jaw incisor

strong

regional

strong expression: see section 09 13

upper jaw molar

strong

regional

strong expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7972
Entity Detected:	Krt6a, keratin 6A ( MGI:1100845)
Sequence:	sense strand is shown >T7972 CTCTTCAGCTCTGCCCTGCCGTTTCTCTACTTCCCAGCCTTCTCATCTCCAGGAACCATGTCTACCAAAA CCACCATCAAAAGTCAAACCAGCCACCGTGGCTACAGTGCCAGCTCAGCCAGAGTGCCTGGGCTCAACCG CTCTGGCTTCAGCAGTGTGTCCGTGTGCCGCTCCCGGGGCAGCGGTGGCTCCAGTGCAATGTGTGGAGGA GCTGGCTTTGGCAGCAGGAGCCTCTATGGTGTGGGGAGCTCCAAGAGGATCTCCATCGGAGGGGGCAGCT GTGGCATTGGAGGAGGCTATGGCAGCCGATTTGGAGGAAGCTTCGGCATTGGAGGTGGAGCTGGTAGTGG CTTTGGCTTCGGTGGTGGAGCTGGCTTTGGTGGTGGCTATGGGGGAGCTGGCTTCCCGGTGTGCCCACCT GGAGGCATCCAAGAGGTCACCATCAACCAGAGCCTCCTCACACCCCTGAACCTGCAAATTGACCCCACCA TCCAGCGGGTCAGGACTGAGGAGAGGGAGCAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGA CAAGGTGCGGTTCCTGGAGCAGCAGAACAAGGTCCTGGACACCAAGTGGGCCCTGCTGCAAGAGCAGGGC ACCAAGACCGTGAGGCAGAACCTGGAGCCTATGTTTGAGCAGTACATCAGCAACCTCCGCAGACAGCTGG ACAGCATCATTGGAGAGAGGGGTCGCCTGGACTCAGAGCTGAGGAACATGCAGGACACAGTGGAGGACTA CAAGAGCAAATATGAAGATGAAATCAACAAGCGCACAGCAGCAGAGAATGAATTCGTGACCCTGAAGAAG GATGTAGATGCTGCCTACATGAACAAAGTTGAACTGCAAGCCAAGGCAGACAGTCTAACAGATGATATCA ACTTCTTGAGAGCTCTCTATGAAGCAGAACTGTCTCAGATGCAAACTCACATCTCAGACACATCTGTGGT CCTCTCCATGGACAACAACCGTAGCCTGGACCTGGACAGCATCATCGCTGAGGTCAAGGCCCAGTATGAG GACATTGCTCAGAGAAGTCGGGCTGAAGCTGAGTCCTGGTACCAGACTAAATATGAGGAGCTGCAGGTCA CAGCTGGCAGACATGGGGACGACCTACGCAACACCAAGCAGGAGATTGCTGAGATCAACCGCATGATCCA GAGGCTGAGATCTGAGATCGACCACGTTAAGAAGCAGTGTGCCAACCTGCAAGCTGCTATTGCTGATGCT GAGCAACGTGGGGAGATGGCCCTGAAGGATGCCAGGGGCAAGCTGGAAGGGCTGGAGGATGCCCTGCAGA AGGCCAAACAGGACATGGCCAGGCTGCTGAAGGAGTACCAGGAACTCATGAATGTCAAGCTGGCCCTGGA TGTGGAAATTGCCACCTACAGGAAGCTGCTGGAAGGAGAGGAGTGCAGGTTGAATGGTGAAGGTGTTGGA CCAGTCAACATCTCTGTGGTGCAGTCCACCGTGTCCAGCGGCTATGGCAGTGCCGGGGGTGCCAGCAGCA GCTTAGGCCTGGGTGGAGGCAGCAGCTACTCCTATAGCAGCAGCCATGGCCTTGGAGGTGGCTTCAGTGC TGGCAGTGGCAGAGCCATCGGAGGTGGCCTCAGCTCTTCTGGTGGCCTCAGCTCTTCTACCATCAAATAC ACCACCACCTCCTCCAGCAAGAAGAGCTACAGGCAGTGAATTCTGTCACCAAGAGCTTGTCTCTGGTCCC AGATGTCATGGCTGCAGAATCCCGTGCTCAGAGCCCCGAGTTCAGGGGCTTCTCCTCCCTGGACCCCACC TCTGCTCCCTTCTTGGGACTGAGGAGGCTGTGTCATTTTGCTCATATTTCTGTCCCCATGGGTCCCCACT GCTCATCTCTTTATAGTCATCCTGTGAGCTTACATCACAATTCACTCACATTTGGTGCTTCATGTTGTAT TTGGTTGCCAGGCTCCTGCCTCCCTACCTCTGTCTCTGAGGCTGCCTGTGACAGGGTGTTTCCGACACCT TCATTTTTGAAATCATTGTCTGGGTCCTACTCAAGTAATGAGCAGCTCCCTGTGAGTTTCTAATGGCCTG AGAAACCCCATCTCTCAACATCATAACCCTCCCTGTCAGTAACTGTGACTGCCCCGTCACTGGTCCTGTG ATGTAAGTTTCTGCTCATGTGATGTCTTTGCTTTCCTTGATGCTCTTGGCTTCCTCGTAATTTCTAAATA AAGCAGGTTTATACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30141946 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30141946 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11320 same embryo
	EMAGE:11323 same embryo
	EMAGE:11321 same embryo
	EMAGE:11322 same embryo
	EMAGE:11319 same embryo
	EurExpress:euxassay_006577 same experiment
	MGI:4825841 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source