EMAGE mouse gene expression spatial database

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EMAGE:11470

Entpd3 ectonucleoside triphosphate diphosphohydrolase 3 ( MGI:1321386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006856. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006856_01	euxassay_006856_02	euxassay_006856_03	euxassay_006856_04

euxassay_006856_05	euxassay_006856_06	euxassay_006856_07	euxassay_006856_08	euxassay_006856_09

euxassay_006856_10	euxassay_006856_11	euxassay_006856_12	euxassay_006856_13	euxassay_006856_14

euxassay_006856_15	euxassay_006856_16	euxassay_006856_17	euxassay_006856_18	euxassay_006856_19

euxassay_006856_20	euxassay_006856_21	euxassay_006856_22	euxassay_006856_23	euxassay_006856_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11470_wholemount_strong.wlz

11470_wholemount_moderate.wlz

11470_wholemount_weak.wlz

11470_wholemount_possible.wlz

11470_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11470_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 03 04

fibula

strong

regional

strong expression: see section 01 02

tibia

strong

regional

strong expression: see section 01 02

femur

strong

regional

strong expression: see section 03 04 05 06 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 19 20

vagus x ganglion

strong

regional

strong expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21

trigeminal v nerve

strong

regional

strong expression: see section 10 11 17 18

anterior naris

moderate

regional

moderate expression: see section 12 13 14 15 16 18 20

external naris

moderate

regional

moderate expression: see section 13 14 19

stomach

strong

regional

strong expression: see section 03 04 05 06 07

lower lip

strong

regional

strong expression: see section 14 15 16 17 moderate expression: see section 13 18 19

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24

upper lip

strong

regional

strong expression: see section 14 16 moderate expression: see section 10 11 12 13 18 19

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23 24

bladder

strong

regional

Expression in the fundus region.

urethra of male

strong

regional

strong expression: see section 14 15 16

left lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

trachea cartilaginous ring

strong

regional

strong expression: see section 14 15

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 23 24 moderate expression: see section 07 08 09 10 11 19 20 21 22

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

strong

regional

strong expression: see section 03 04 23 24

esophagus lumen

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9216
Entity Detected:	Entpd3, ectonucleoside triphosphate diphosphohydrolase 3 ( MGI:1321386)
Sequence:	sense strand is shown >T9216 CCGCTTTTCAGCAACCCGCAGCGAAAGCAGCGCCCAGCCCGGAGAGAAGATGTTTACCGTGATGACCCGC CAGCCATGTGAACAGGCAGGCTTCAGGGCCCTCTCGAGGACTCCTGCCATCGTCACCTTGGTGGTCCTGC TTGTGAGCATTGTGGTACTTGTGACACTTACACTCATCCAGATCCGCCACCCACAGGTTCTCCCTCCGGG GCTGAAGTATGGCGTCGTGCTCGATGCTGGCTCTTCCAGAACCACTGTCTACGTGTATCAGTGGCCGGCA GAGAAGGAGAATAACACAGGAGTGGTCAGCCAAACTTTCAGATGCAGTGTGAAAGGCTCTGGGATCTCCA GCTATGAGAATAACCCCCAAGATGCCCCCAAAGCCTTTGAGGACTGTATACTAAAGGTCAAGGAACAGGT CCCAGAGCACCTCCACGGATCCACCCGCATTTACCTGGGAGCTACAGCTGGGATGCGCTTGCTGAGGTTG CAGAATGAGACAGCAGCTCGTGAAGTCCTTGAGAGCATCCAAAGCTACTTCAAGTCCCAGCCTTTTGATT TTAGGGGTGCTCAAATCATTTCTGGGCAAGAGGAAGGGGTGTATGGATGGATTACAGCCAACTATATAAT GGGAAATTTCCTGGAGAAGAACCTGTGGCACATGTGGGTGCACCCGCACGGAGTCGACACCACAGGAGCC CTGGATTTAGGTGGCGCCTCCACCCAGATATCCTTCGTGGCCGGGGAGAAGATGGAGCCGAACGCCAGCG ACACGGTGCAGGTGTCTCTGTACGGCTACACGTACACTCTCTACACACACAGCTTCCAGTGCTATGGCCA GAATGAAGCAGAGAAGAAGTTCCTGGCCATGCTTCTACAGAGCCCTTCCACGGAAGCCAACATCAGCAAC CCCTGCTACCCTCAGGGCTACAGTACCGCCTTCACCTTGGGCCATGTGTTTGGCAGCCTGTGCACAGAGA AGCAGAGGCCAGAGAGCTACAACTCCAGTAAGAGCGTCACCTTCATGGGAACTGGTGACCCACGACTGTG CAGGGAGAAGGTGGCTTCTGTGTTTGACTTCAATGCTTGCCAAGAGCAAGACGCCTGTTCCTTTGATGGC ATTTACCAGCCCAAGGTTCAAGGGCCATTTGTGGCGTTCGCAGGCTTCTACTACACAGCCAGTGCGCTAA ACCTCTCAGGAAGCTTCTCCCTTACCTCCTTCAATGACAGCAGCTGGGACTTCTGCAGACACACTTGGAG CGAGCTCCCAGCCCTGCTCTCCAGATTTGATGAGACGTATGCCCGGTCCTACTGCTTCTCAGCCCACTAC ATCTACCACTTGCTCGTAAATGGATACAAGTTCACTGAGGAGACTTGGCCTCAGATACGCTTTGAAAAAG AAGTGGGGAACAGCAGCATCGCCTGGTCCCTTGGCTACATGCTCAGCTTGACCAACCAGATCCCAGCTGG AAGTCCCCTGATCCATCTACCCATACAGCCACCGGTGTTTATGGGAGTCCTGGCCTTCTTCACAGCTATC GCCTTGCTGTGCCTGGCATTTCTTCTGTATCTATGTTCATCATTCAGGACAAAGGAGCGCTCTGAGAATG CCTTCGACCAAGCAGTGGATTCTGATTGAGCCTTCCAAGCAGCACCTGTCACCCCAGGCTGCCAGAACCC ACCAGAGTGACACCACACAGTGTGAGTGAAGTGGCTGCCACCAGGACCTGTCCCGTGCTTAAATGCCAAG GTCATGTGCCTCTCAGCCACTGATTCCTGCCAGAGCACCTCCTGGGAGTCCCTCATCCACCCAGTGGGGA CTGCCTTTGCAATCCTCCCTACCTGCTTGCTGATCCACTGGGAATCAAGGAGACACTGGCTGTTGGTGTC AGGGGCTTCACTCTGTCTACTCCAGAGGAAGTTGAGAATATAATTTAATGCAGAAGAATTGACCACGGGC TCGGTTTCCCTTTTCTCTCCCTCCGTGCTCTTCCTAGCAAGCTGCCCATGAAGGCTCCGACTCTGATACT TCACCCCACCCTGTGGCTTTGACCTAGAGTTCCTTTGCAAGACCTTCCTGTATTTTAGTTGAGTCGAGCC CTATCTCAAGTCACTAATGCTTTTTTTTAGCAATCTCATAAGCATTAAGCTCCCTGACTGGTAGCTAGTA GCATTTAGTGGGGAAGATGATGGCATTTTGAGACAGTTTCATGGAGCCCATGCTAGCTTCCTGCCCACTA TGTAGCCAAGAGCAGCCTTGATATCTGTTCTTTTCCTGAGGGCAGGGATTATGGGTTGTGAGGTCCTCGG GGTGGAACCCCAGAGCTTCATGCATTCTAGGCAACTATTTGACCAACCGAGTCACACGCCCAGCCTAAGA AGATTCAGCCTCAAGGACAGATGGCCTATGTCATGTAGGCAGACCTGAAGCACAAAGACACACAGAAGTC CCTAAATACAGAGTCCCTATTCTTATTTTGGAGTGCCCACTTGGAGCTCTGGTCATGAGGTTGCTACTAG GCATGTGTCCTCCTTGTGTAACTGTCTCCCTGCTGTGTCCTCTTGTAAATCACACCCAGTATGGACTGTA CTGGGGGGGGGGGCAGTGAGGGGATCTCATTGACCCGGCAAAGCTCCTGCTCAGGGTGCCTTTGGGCTAT AAACATGGGGTGTGCTTTTTTCCCCACTGCTCCCACAGGTATACAGGATCCTGTCCAAAGACATCCCTAC ATTTCTCCCTGCAGAGTTGCTACACATCTTGACTTTGGGAAAAGAGAAGGCAGTCTCAGGAATATTTGCC TCCAAAGTGGGATGGGAAAGGTCTTCAAGTTCAGTAGCTACAAGCTACAAATCAATGCTGTGTCTCATGG CTGTTTGCTGGCTCCATATGGAATAAATTCTTATTTTTATTTTGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831925 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831925 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11471 same embryo
	EMAGE:11468 same embryo
	EMAGE:11469 same embryo
	EMAGE:11473 same embryo
	EMAGE:11472 same embryo
	EurExpress:euxassay_006856 same experiment
	MGI:4824565 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source