EMAGE mouse gene expression spatial database

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EMAGE:11601

Tmem97 transmembrane protein 97 ( MGI:1916321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006766. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006766_01	euxassay_006766_02	euxassay_006766_03	euxassay_006766_04

euxassay_006766_05	euxassay_006766_06	euxassay_006766_07	euxassay_006766_08	euxassay_006766_09

euxassay_006766_10	euxassay_006766_11	euxassay_006766_12	euxassay_006766_13	euxassay_006766_14

euxassay_006766_15	euxassay_006766_16	euxassay_006766_17	euxassay_006766_18	euxassay_006766_19

euxassay_006766_20	euxassay_006766_21	euxassay_006766_22	euxassay_006766_23	euxassay_006766_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:11601

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11601_wholemount_strong.wlz

11601_wholemount_moderate.wlz

11601_wholemount_weak.wlz

11601_wholemount_possible.wlz

11601_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11601_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24 moderate expression: see section 04

radius

moderate

regional

moderate expression: see section 01

ulna

moderate

regional

moderate expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24

hand

moderate

regional

moderate expression: see section 02

forelimb digit 1

moderate

regional

moderate expression: see section 01

forelimb digit 2

moderate

regional

moderate expression: see section 01

forelimb digit 3

moderate

regional

moderate expression: see section 01

forelimb digit 4

moderate

regional

moderate expression: see section 01

forelimb digit 5

moderate

regional

moderate expression: see section 01

hindlimb digit 1

moderate

regional

moderate expression: see section 01 02 03 23 24

hindlimb digit 2

moderate

regional

moderate expression: see section 01 02 03 23 24

hindlimb digit 3

moderate

regional

moderate expression: see section 01 02 03 23 24

hindlimb digit 4

moderate

regional

moderate expression: see section 01 02 03 23 24

hindlimb digit 5

moderate

regional

moderate expression: see section 01 02 03 23 24

fibula

moderate

regional

moderate expression: see section 01 02 03 23 24

tibia

moderate

regional

moderate expression: see section 01 02 03 23 24

femur

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22 23

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 17 18 19

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 19 20 21

naris

strong

regional

strong expression: see section 10 11 12 13 14 15

viscerocranium

strong

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 15 16

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 10 11 15 16

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial skeleton

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24 moderate expression: see section 11 12 13 14 15 16 17

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

clavicle

strong

regional

strong expression: see section 09 10 11 17 18 19

scapula

moderate

regional

moderate expression: see section 05 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7363
Entity Detected:	Tmem97, transmembrane protein 97 ( MGI:1916321)
Sequence:	sense strand is shown >T7363 CAGCTCGGGCGCGGGGCTTCTAACCACGTAGCAGGCTTCTATCACCGGCTTGCCAGTCGTAGCCCAACGG ACAGGAGGGAACATGGGGGCCCTGGCAGCTAGGCGCTGTGTCGAGTGGCTCTTGGGCCTCTACTTCGTCT CGCACATCCCCATCACGCTGTTCATCGACCTGCAGGCGGTGCTGCCGCCCGAACTATACCCGCAGGAGTT CAGCAACCTGTTGCGGTGGTACTCAAAGGAGTTCAAAGACCCTCTGATGCAGGAGCCCCCAGTGTGGTTC AAGTCCTTCCTGCTCTGTGAGCTTGTGTTCCAGCTGCCTTTCTTTCCCATTGCGGCATATGCCTTCTTCA AAGGAAGCTGCCGGTGGATCCGAATCCCTGCAATCATCTATGCAGCTCACACCATAACGACTTTAATTCC AATCCTCTACACACTCCTGTTTGAGGATTTCTCTAAAGCCGTTGCTTTCAAGGGACAAAGACCCGAGAGT TTCCGTGAACGACTGACCCTCGTAGGTGTCTACGCCCCCTATTTAATAATCCCCCTCATACTCCTCCTGT TCATGCTTCGGAACCCGTACTACAAGTATGAGGAGAAAAGAAAGAAAAAATAGGGACGGCCACCAGCCTG GTGGGGAAGACCCCACAGCACAGTTGCCTTTTGGACAACACAGGAAAACTGCCCCAAACATGTTTCAGCA GCATTTGAAACACCAACAGCAGTGCATACAAACAAGACAGTGGTGGGAGACGTGCCAGGCCTTCACCTTT CAGCAGGGGTGTGACCAAGTTACAGCAAAATGGTGCCAAAGCGCTACCACAGCACTGCTAAAAAGGCCAG GTGGGAAGCCGGTTAGCTCCTGACCTGGAATGCACACACCTGACCAAGTCCTCACCTCGACCTTGATAGT CAACAAATGAGCAGTATTTTGTCTCTTTAGCACTTTTTAACATGGTTTCCTGCCCACACCTCCACCTCTG GGAGAAAAATATATTATAGCCTCACATGAGTCCAAGCCAGAAATGGGTTACAGGGAAGGAAGGTGCCTCC ATTTCTATCTTGAACAGATAGCCTGCTCTGGGTTATTCTGGTGCTGGTTCTATGAACGGCTCTCATGTCC CTACTCTGGCACCTCACCCCACTGGGCTTCCTAGTACACAGGCTTGTGAGGGTGACTGGACTGTCCAGTG TATGTCTGCTGCTTGTTCCACTGACTCTACAGAAACAAAACCCGGTGGGTGCTAGACAGTGCTGGCTCTG CTTGCAAGCCATGTTGCTGTGGTTGTTACTTATGTGTCTGGTCCAAATAAAGGCAGCTGCTGATTTGTTG TTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4192256 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4192256 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11600 same embryo
	EMAGE:11597 same embryo
	EMAGE:11596 same embryo
	EMAGE:11598 same embryo
	EMAGE:11599 same embryo
	EurExpress:euxassay_006766 same experiment
	MGI:4828822 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source