EMAGE mouse gene expression spatial database

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EMAGE:11679

Gyltl1b glycosyltransferase-like 1B ( MGI:2443769)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006772. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006772_01	euxassay_006772_02	euxassay_006772_03	euxassay_006772_04

euxassay_006772_05	euxassay_006772_06	euxassay_006772_07	euxassay_006772_08	euxassay_006772_09

euxassay_006772_10	euxassay_006772_11	euxassay_006772_12	euxassay_006772_13	euxassay_006772_14

euxassay_006772_15	euxassay_006772_16	euxassay_006772_17	euxassay_006772_18	euxassay_006772_19

euxassay_006772_20	euxassay_006772_21	euxassay_006772_22	euxassay_006772_23	euxassay_006772_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11679_wholemount_strong.wlz

11679_wholemount_moderate.wlz

11679_wholemount_weak.wlz

11679_wholemount_possible.wlz

11679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 14 15

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

thyroid gland

strong

regional

strong expression: see section 10 11 15

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 22 23 24

inner ear

strong

regional

strong expression: see section 01 02 03 04 05 18 19 20 21 22 23 24 moderate expression: see section 06 07 08 16 17

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

anterior naris

strong

regional

strong expression: see section 14 15 16 17 18 19

external naris

strong

regional

strong expression: see section 14 15 20

posterior naris

strong

regional

strong expression: see section 14 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

naso-lacrimal duct

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 20 21 22 23

tongue epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

frenulum

strong

regional

strong expression: see section 16

stomach

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 12 13 14 17 18 19

lower jaw molar

strong

regional

strong expression: see section 08 09 20 21

upper jaw incisor

strong

regional

strong expression: see section 13 14 15 17 18 19

upper jaw molar

strong

regional

strong expression: see section 08 20 21

bladder

strong

regional

strong expression: see section 14 15

metanephros

strong

regional

strong expression: see section 06 07 08 09 10 11 17 18 19 20 21

urethra of male

strong

regional

strong expression: see section 15 16

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13

right lung

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8169
Entity Detected:	Gyltl1b, glycosyltransferase-like 1B ( MGI:2443769)
Sequence:	sense strand is shown >T8169 GGCACGAGGGTGATGGAGCCCGCAGCCCGGGGTCGCGCCCCGCCCTGAGTCCCACAGTCATGCTGCCCCG AGGTCGTCCCCGGGCAATGGGGGCCGCCGTGCTGCTGCTGCTGCTGTTGCTAGTGGTTGGCTTCTTCCTG TTCGGCCGGGACCCGGATTATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTACAGGAGTCGCA ACCTCTCCGCCTCCAGCCCGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTGGCTATCGTGTG TGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCCTGCTATTCTACAGGAAAAATCCG CTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCGAACATGGATGG TGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCCCTGGTCTCCTGGATCCCCAACAA ACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCATCCTGCCTCCCAGCCTGGCCCGA GTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGAGCTCTGGGCACTCTTTGATCATT TTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGGTACCTGGGCAACCTCTGGAAGAA CCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTGTGGCTGGACAGGCTC CAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGAGCTGCTCACTCTGATGGCTACTT CCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCCCATCTGGTGCACCCCCTGCCCTG TGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCTACCTGGAAGCAGCTGACCTCAAA GTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCACGCAGAATTCTTCCGTAATCTGC ACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTCTTTGGATGCCCCAACCAGTTCCC TCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGGAAGAGCCCTGCTTTGAGTTCCGC CAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCACCAGCCGCCACCTCCCCAGCCTC ACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTGTGCAGGCACTG GCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTCAACAATTTCTTCATTTTGTGGAA ACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGTGTACCGGGACGGTCCACTCTATC CAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTCAGTGATATTGA CTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCGAGCAGCTGGAGCTGGACAGTCGG CGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCGGTTCAGCTTCCCAAACTCTAAGG CAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGGTACCACGAGTGGCCACAGGGTCA CTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACAGTGTGCAGTGGTCAGCTGACTAT GAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCGCTTTGTGGGATTTGGCTGGAACA AGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAGGCCTTCTCTAT CCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCAGCCCCACCTACCGCAACTGTCTC CAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGGGTCTGCAGCCCTGAAATACCTCA CTGCCCTGCAGCAGGCCCGAAGTCGGGCCTGAGCTTACCCCGCTGCCCTAGCTATGGCTTACACATTCCC TGTAGAGGCTGACCAGGGTGTCCACCCTTACTGCTATTTAAGTTATTTAAAATTTTTGTCAGAAGGGGCA GAGTGGAGCATTTGTGGGGTTCCTGGTTCCTTCTGCTGATCCACACTGGGATCCAGCCCTCCTTTGCCTC AGCTGGTTTGGGGCTAGTCTAATTCTCTTATATAATAAAAATTTTCAATTTTTTTTATCTTAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5057489 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5057489 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11677 same embryo
	EMAGE:11676 same embryo
	EMAGE:11678 same embryo
	EMAGE:11675 same embryo
	EurExpress:euxassay_006772 same experiment
	MGI:4825291 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source