EMAGE mouse gene expression spatial database

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EMAGE:11708

Gata3 GATA binding protein 3 ( MGI:95663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006921. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006921_01	euxassay_006921_02	euxassay_006921_03	euxassay_006921_04

euxassay_006921_05	euxassay_006921_06	euxassay_006921_07	euxassay_006921_08	euxassay_006921_09

euxassay_006921_10	euxassay_006921_11	euxassay_006921_12	euxassay_006921_13	euxassay_006921_14

euxassay_006921_15	euxassay_006921_16	euxassay_006921_17	euxassay_006921_18	euxassay_006921_19

euxassay_006921_20	euxassay_006921_21	euxassay_006921_22	euxassay_006921_23	euxassay_006921_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11708_wholemount_strong.wlz

11708_wholemount_moderate.wlz

11708_wholemount_weak.wlz

11708_wholemount_possible.wlz

11708_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11708_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 08 09 10 11 16 17 18

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15 16

skin

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

pons mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 11 14 15 16 17

cervical ganglion

strong

regional

strong expression: see section 09 10 17 18

thoracic ganglion

strong

regional

strong expression: see section 12 13 14 15

inner ear

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23

perioptic mesenchyme

strong

regional

strong expression: see section 02 03 04 05 06 07 08 23 24

nasal cavity

strong

regional

strong expression: see section 13 14 15 17 18 moderate expression: see section 09 10 11 12 16 19 20 21 22 23

urinary system

strong

spotted

strong expression: see section 03 04 05 06 07 20 21 22 23

bladder

strong

regional

strong expression: see section 14 15

kidney calyx

strong

regional

strong expression: see section 06 07 08 09 10 11 16 17 18 19 20 21

urethra of male

strong

regional

strong expression: see section 14 15 16

larynx

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 12 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9151
Entity Detected:	Gata3, GATA binding protein 3 ( MGI:95663)
Sequence:	sense strand is shown >T9151 TTTTCTTTCCTCCCTAAACCCTCCTTTTTGCTCTCCTTTTCTATACCCTTAACTGCAAACAAACCATTAA ACGACCCCTCTCCTGGGCCTCCGACGGCAGGAGTCCGCGGACCTCCCAGGCCGACAGCCCTCCCTCTACC CGCGAGGGTTCCGGGCCGGGCGAGAGGGCGCGAGCACAGCCGAGGACATGGAGGTGACTGCGGACCAGCC GCGCTGGGTGAGCCACCATCACCCCGCGGTCCTCAACGGTCAGCACCCAGACACGCACCACCCGGGCCTC GGCCATTCGTACATGGAAGCTCAGTATCCGCTGACGGAAGAGGTGGACGTACTTTTTAACATCGATGGTC AAGGCAACCACGTCCCGTCCTACTACGGAAACTCCGTCAGGGCTACGGTGCAGAGGTATCCTCCGACCCA CCACGGGAGCCAGGTATGCCGCCCGCCTCTGCTGCACGGATCTCTGCCCTGGCTGGATGGCGGCAAAGCC CTGAGCAGCCACCACACCGCCTCGCCCTGGAACCTCAGCCCCTTCTCCAAGACGTCCATCCACCACGGCT CTCCGGGGCCTCTGTCCGTTTACCCTCCGGCTTCATCCTCTTCTCTGGCGGCCGGCCACTCCAGTCCTCA TCTCTTCACCTTCCCGCCCACCCCGCCGAAAGACGTCTCCCCAGACCCGTCGCTGTCCACCCCGGGATCC GCCGGGTCGGCCAGGCAAGATGAGAAAGAGTGCCTCAAGTATCAGGTGCAGCTGCCAGATAGCATGAAGC TGGAGACGTCTCACTCTCGAGGCAGCATGACCACCCTGGGTGGGGCCTCATCCTCAGCCCACCACCCCAT TACCACCTATCCGCCCTATGTGCCCGAGTACAGCTCTGGACTCTTCCCACCCAGCAGCCTGCTGGGAGGA TCCCCTACCGGGTTCGGATGTAAGTCGAGGCCCAAGGCACGATCCAGCACAGAAGGCAGGGAGTGTGTGA ACTGCGGGGCAACCTCTACCCCACTGTGGCGGCGAGATGGTACCGGGCACTACCTTTGCAATGCCTGCGG ACTCTACCATAAAATGAATGGGCAGAACCGGCCCCTTATCAAGCCCAAGCGAAGGCTGTCGGCAGCAAGG AGAGCAGGGACATCCTGCGCGAACTGTCAGACCACCACCACCACCCTCTGGAGGAGGAACGCTAATGGGG ACCCGGTCTGCAATGCCTGTGGGCTGTACTACAAGCTTCATAATATTAACAGACCCCTGACTATGAAGAA AGAAGGCATCCAGACCCGAAACCGGAAGATGTCTAGCAAATCGAAAAAGTGCAAAAAGGTGCATGACGCG CTGGAGGACTTCCCCAAGAGCAGCTCCTTCAACCCGGCCGCTCTCTCCAGACACATGTCATCCCTGAGCC ACATCTCTCCCTTCAGCCACTCCAGCCACATGCTGACCACACCGACGCCCATGCATCCGCCCTCCGGCCT CTCCTTCGGACCTCACCACCCTTCCAGCATGGTCACCGCCATGGGTTAGAGAGGCAGAGCCCTGCTCCAC ATGCGTGAGGAGTCTCCAAGTGTGCGAAGAGTTCCTCCGACCCCTTCTACTTGCGTTTTTCGCAGGAGCA GTATCATGAAGCCCGAAAGCGACAGATCTGTGTTTTTGAAGGCAGAAAGCAAAATGTTTGCTTCTTTTTT CAAAGGAGCTCGAGGTGGTGTCTGCATTCCAACCACTGAATCCGGATCCCATTTGTGAATAAGCCATTCA GACTCATATTCCCTATTTAACAGGGTCTCTAGTGCTGTGAAAAAAATATTGCTGAACATTGCATATAACT TATATTGTAAGAAATACTGTACATTTGAGGAAGACTTTATTGTACCTGGATAGCTGTAAGAAAGGCATGA AGGACGCCAAGAGTTTTAAGGAATATAGGGGGATTAAAGTATGGAGATACAGAAGAAACCACTAAGTCTG ATGTCCAAATGGGCACACTGTCAGTTTTGTTTCCCTTCAGTTGTTTGATGCATTTAAAAAAAAAAAAAAG AAAGAAAAAGAAAAAAAGGGGGGGGGGGGAGAAAAAAATAAATTAAAAAAAAAAAAAAAAAAAAGAAAAG AAAGAAAAATCTAAGAAAAAAAAAAAAAAGGTTGTAGGCAAATCATTTGTTCCAGGCTGTGAGCCTGTGC AAAAGAGATTTCAGATCTGGGCAATGGGTGTGTGATCTCACCCACTGAAGATCTGAGAATGTCATGGCTA GGCCTACATGCTCTGTGAATCAGTCCCTGTAATTGTTGTTTGTATGTATAATTCAGAAGCACCAAAATAA GAAAAGATGTAGATTTATTTCATCATATTATACAGACTGAATTGTTGTATAAATTTATTTACTGCTAGTG TTAGGAACTGCTTTTTTTTTTTTTTTGGTTTTAATGTTTTTTTTTTTTTTGTTTTTTGTTTTTTTTTTTC TTTCTCTCTGGATTTTTGGTTGAATAAACTAGATTGCTTTCAGTTGACTTAAGGTGGATGTACTCTGGAG GGTTTATTTTTCCTTTTATTATTATTTTTGATGGTATTTATTAAATAGCTTCTATGGGCCCGGCGGTACC TGTCTTTTTCGTCACTTTTCTTGCAGCCTAAACTATGAAGGTAGCAGCGTACCAGCTACCAACATGCATG TCAGAGACCCGGCCACTCACAGGCCTGGTCCTGAGAGCCACCTGGCTGACTGTTAGCCCCTGTGTGTTCT GTATTAGTGATCACTGCCTTTAAACAGTCTGTTGGAATAATACTATAAAAATAATAATAAAGTTAAAATA TTTTAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826352 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826352 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11706 same embryo
	EMAGE:11705 same embryo
	EMAGE:11703 same embryo
	EMAGE:11707 same embryo
	EMAGE:11704 same embryo
	EurExpress:euxassay_006921 same experiment
	MGI:4825003 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source