EMAGE mouse gene expression spatial database

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EMAGE:11724

Edar ectodysplasin-A receptor ( MGI:1343498)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006696. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006696_01	euxassay_006696_02	euxassay_006696_03	euxassay_006696_04

euxassay_006696_05	euxassay_006696_06	euxassay_006696_07	euxassay_006696_08	euxassay_006696_09

euxassay_006696_10	euxassay_006696_11	euxassay_006696_12	euxassay_006696_13	euxassay_006696_14

euxassay_006696_15	euxassay_006696_16	euxassay_006696_17	euxassay_006696_18	euxassay_006696_19

euxassay_006696_20	euxassay_006696_21	euxassay_006696_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11724_wholemount_strong.wlz

11724_wholemount_moderate.wlz

11724_wholemount_weak.wlz

11724_wholemount_possible.wlz

11724_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11724_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 02 03 04 20 weak expression: see section 01 17 18 19

cranial musculature

weak

regional

weak expression: see section 01 02 03 04

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 22

telencephalon ventricular layer

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 01 02 03 04 05 09 10 19 20 21 22

trigeminal v ganglion

weak

single cell

weak expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

otic capsule

weak

regional

weak expression: see section 05 06 07 08 16 17 18

lens

moderate

regional

moderate expression: see section 01 02

viscerocranium

moderate

regional

Expression in the turbinate bone.

lower jaw molar

moderate

regional

moderate expression: see section 06 07 20 21 weak expression: see section 08 09 19

upper jaw molar

moderate

regional

moderate expression: see section 06 07 20 21 weak expression: see section 08 09 18 19

trachea cartilaginous ring

moderate

regional

moderate expression: see section 12 13

axial skeleton tail region

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8112
Entity Detected:	Edar, ectodysplasin-A receptor ( MGI:1343498)
Sequence:	sense strand is shown >T8112 CTTCAAAATAGAAAGTTAGTTGCGCTGGCAGCAGAGGTGTGCCTGGCCGCTGTCACCCGGCTGGCCCCAG GATTGTGGAGCTCTGCTTTTGAGAGGACACCGACGGACGCCTGTGAAGCCTGCCCCCCATCCCTTACCTG CTCGCCTTCTCCGTAGACCCATCTTCTGCTGGGAAAAGCTAACCTCATTCGGGTACCAGGTGTACTTCCA AGAGATCATGGCCCACGTCGGGGACTGCAAATGGATGTCCTGGCTCCCAGTGCTGGTGGTGTCTCTGATG TGCTCAGCCAAGGCGGAGGACTCCAACTGTGGTGAGAACGAATACCACAACCAGACTACCGGGCTGTGCC AGCAGTGTCCTCCATGCAGACCAGGGGAGGAGCCCTACATGTCCTGTGGATACGGCACTAAAGACGACGA CTATGGCTGTGTGCCCTGCCCTGCAGAGAAGTTCTCCAAAGGAGGTTATCAGATATGCAGGCGCCACAAA GACTGTGAGGGCTTCTTCCGGGCCACTGTGCTGACACCAGGAGACATGGAAAACGACGCTGAGTGTGGCC CATGTCTCCCTGGCTACTACATGCTGGAAAACAGACCCAGGAACATCTATGGCATGGTCTGCTACTCCTG TCTCTTGGCACCTCCCAACACCAAGGAATGTGTGGGAGCCACTTCTGGGGTTTCAGCACACTCATCCAGC ACTTCCGGTGGCAGCACCTTGTCTCCCTTCCAGCATGCTCACAAAGAGCTCTCAGGCCAAGGACACCTGG CCACCGCCCTGATTATTGCCATGTCTACGATCTTCATCATGGCCATTGCCATCGTCCTCATCATCATGTT CTACATCATGAAGACTAAGCCGTCAGCTCCAGCCTGCTGTAGCAGTCCCCCAGGAAAGAGCGCAGAAGCC CCAGCTAACACACACGAGGAGAAAAAAGAGACCCCAGACAGTGTGGTGACGTTCCCTGAGAATGGTGAGT TCCAGAAGCTGACAGCAACACCCACAAAGACCCCCAAAAGTGAGAATGATGCCTCCTCTGAGAACGAGCA GTTGCTAAGTCGCAGTGTGGACAGTGATGAAGAGCCAGCCCCGGACAAGCAGGGGTCCCCAGAGCTATGT CTGCTGTCGCTAGTTCACCTGGCCAGGGAGAAGTCTGTGACCAGTAACAAGTCTGCTGGGATCCAGAGCC GGAGGAAAAAGATACTGGATGTGTATGCCAACGTGTGCGGTGTTGTTGAAGGTCTCAGCCCCACCGAGTT GCCGTTTGACTGCCTTGAGAAGACAAGCCGAATGCTCAGCTCTACATACAACTCTGAGAAGGCGGTCGTG AAAACATGGCGCCACCTTGCCGAGAGCTTTGGGCTGAAGAGGGATGAGATTGGGGGCATGACTGATGGCA TGCAGCTCTTTGACCGCATCAGCACCGCGGGCTACAGCATCCCAGAGCTGCTCACAAAGTTGGTGCAGAT CGAGCGGCTGGATGCTGTGGAGTCCTTGTGTGCAGACATATTGGAGTGGGCTGGGGTTGTACCACCTGCC TCCCCACCCCCAGCTGCGTCCTGAAGAGTTGTCTTGGACTGTCTTCCCTGGGACCAGCTGGGGATCCAAT GAAGTCACGACCGACAGCTGTGAGTGATGCTATCAGACTGCCAAAACTCAAGGCATTTCCTGGTGGGTCA CTGTATTCCTTAGGCTGCCCTAAGTAGTTCATTGAGCACTCAAATGAACCAAACCATGTGGGAAGGACAC AGGTGAAGAATCTGATCCTGTCTTTTAAGGAGGCACTTAGTGAGAGATGGGAGCATGGTATAGACCGTGT TACCAAAACATCACCTAGGCAAATGAAGGGATGCTTTTTTAAAAAGTAGCAAAAGTTATAAGGGTGATAG AGTCTCAAGGGGTTGAAAGTGGGAACATCCTAATGAAGAAAATAACTTCAAGGTTTTAGTAAAACGGTTA CAAAGTACAGGCATCCCACATCCTTCATGGCCTCAGACAGAGCATGGTTTGCTGGCATGCCCTATGGTTT CAGAGGTAACTCGACCTGTGTTTGCAGTCACACACCATGGTATAAGCCTTGCAGTCACTAGTGGAAGAAC TCACCATGTGTCACTGACATACAAGGCGTGTGTCTGTCCAGTCACAAGTGTGGGCAGATCTGGCCTCCAG CACCACTCGCAGCCACAGCAGTTACAATGTCAGGCTTGTCTTGCTTCAAAGGGACGTGCGTCCTATCTAG AGAAGGAAATGGTGACTTGCTCAGAGTTTGACCATGCCTGATTCCTGGGTGAGGCTCGAGTGAGTTCAGG CACAACATCCTGAGCCAGTGAGGGGTGGTGCAGCCCGAGACGCAGTACAGAGCTGGGGTCTGAGGTCCTG GGCACTGGGAGAGTCATTCAAAGTGTCTGTCTCCTCCAGACTTAGCTTCTCTTAGCTGAGAGAGGTTGGT CTTCACATCTGTAGTCAGGAATGTTGAGGCTCCAGTGAGCGAAAGGGTGAGTGAAAGAACACGGAGGCAG AAGAGAAGAGGCCAGAGGAGCCTCCATGGGGTAAATACAGTGTGGGTGGCCACAAAAAATGAGAGTCAAG GGAAACGCAGCCGCCATTCTCATATAAGGGCAACTGTAGGACTGAGCGTTTACAGGGCTTATAAACAGCC ATTGCTCTTGGCATATTCTCTTACTGCACCTGTAACTGCCAGGAAATGGGAGCCAAGGAAACTTCCCCGG CAGGGGGTTAATGCCAAGCTGGCTGCTGGGCTCCCTCCCAGAGCGCTGACTGCAGAGAATGCTTCCTTTC AGTAAAGCCTCTGGTTTAGAAGGCGGTTGGGTTTTTTTGTTTTACAAGGCCTATGACTGAACAAAGGCTT TGGAGAGCAATCAGTGGTGTGTTTAAAACCATCAAGCCATTTCCCACCACTGAATATAGACCATACTGTG AGAGGACCATAATTAGGTCCTGAATTTTTAATATGATCATTTTCCTGTGTCTGTCTGTGCAGTGTTTTTT TTTTTTTTTTAAAGAAGGCATTTACTCCATTTTGCAAGTTAAATGTCTGCTTAATTGTCCTTTTAATTCC TGAGACCCTGCAGTGTCCCTTACCCCTGGTCTTCCATAATGACGCTGCGATTCCCCGTAATTAGACTTGT AAATGTCATGCGTGATGAGTGAGCAGGTCGAGGACAGCAGGCTCTTCTCCAACTGTCATTGTGCTGAAGA ATGGGCAGCTGCAGCCAGCGGTGTGGGCTGCCCTCCATTCACACTACTAGGTTTCAAGGCCTGAGGCAGC CAGCATCCTTGTTGTTTCCTTGACTCCCTGCTTGCTGCTTTAGGGGAGCCAGTCCCTTGTCATTTACTTT ACATGGCAATAAATCCTGGAAGGGTTGGTTGGCTTCAGTGTGCTTTGCCAACCAACAAGACCACAGTGAC TCTTGCTGACCAATGCTGGAACTCCACGCTGCCATGTTTGTCTGGAGACTGTTATTATTTTTTCAGTACT TAAAGGTATTTAGTAAACACCCAAGCTAGGTCTGAGGGCCTGAGCCAGTGAAGTCTTAATTGTGAATATT TTATATAATTCTGTTTATGTAACTTATTATATTTTTATAAGCTCAATAAACATATTGATAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30098537 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30098537 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11725 same embryo
	EMAGE:11726 same embryo
	EMAGE:11727 same embryo
	EMAGE:11728 same embryo
	EMAGE:11722 same embryo
	EMAGE:11723 same embryo
	EurExpress:euxassay_006696 same experiment
	MGI:4824469 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source