EMAGE mouse gene expression spatial database

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EMAGE:11726

Rasa3 RAS p21 protein activator 3 ( MGI:1197013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006677. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006677_01	euxassay_006677_02	euxassay_006677_03	euxassay_006677_04

euxassay_006677_05	euxassay_006677_06	euxassay_006677_07	euxassay_006677_08	euxassay_006677_09

euxassay_006677_10	euxassay_006677_11	euxassay_006677_12	euxassay_006677_13	euxassay_006677_14

euxassay_006677_15	euxassay_006677_16	euxassay_006677_17	euxassay_006677_18	euxassay_006677_19

euxassay_006677_20	euxassay_006677_21	euxassay_006677_22	euxassay_006677_23	euxassay_006677_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11726_wholemount_strong.wlz

11726_wholemount_moderate.wlz

11726_wholemount_weak.wlz

11726_wholemount_possible.wlz

11726_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11726_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

olfactory cortex ventricular layer

weak

regional

weak expression: see section 12 13 14 17 18 19

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22 23

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 moderate expression: see section 02 03 04 20 weak expression: see section 07 08 17 18 19

pons ventricular layer

strong

regional

strong expression: see section 05 06 weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23

lower jaw incisor

strong

regional

strong expression: see section 13 16

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23

upper jaw incisor

strong

regional

strong expression: see section 13 14 17

liver left lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

spotted

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8108
Entity Detected:	Rasa3, RAS p21 protein activator 3 ( MGI:1197013)
Sequence:	sense strand is shown >T8108 CAGCATGGCGGTGGAGGAAGAGGGACTGCGGGTCTTCCAGAGCGTGAGGATCAAGATTGGTGAAGCCAAG AATCTTCCTTCCTACCCGGGGCCAAACAAGATGAGGGACTGCTACTGCACTGTGAACCTGGACCAGGAGG AGGTTTTCCGGACCAAAATTGTGGAGAAGTCACTCTGCCCCTTTTACGGAGAGGACTTTTACTGTGAAAT CCCTCGGAGTTTCCGCCACCTGTCCTTTTACATTTTTGATAGAGATGTTTTCCGAAGGGACTCCATCATA GGCAAGGTGGCCATCCAGAAGGAAGACTTGCAGAGGTACCACAACAGGGACACATGGTTCCAGCTGCAGC ACGTGGACGCAGACTCAGAGGTGCAGGGCAAGGTCCACCTGGAGCTGAGACTAAGTGAGGTCATTACGGA CACTGGTGTCGTCTGCCACAAACTCGCTGCACGCATCTTCGAGTGCCAGGGTCTCCCCATTGTGAACGGG CAGTGTGACCCTTACGCCACAGTGACGCTGGCCGGACCCTTCAGGTCTGAAGCAAAGAAAACGAAGGTGA AGAAGAAAACCAACAACCCCCAGTTTGACGAGGTGTTTTATTTTGAGGTGACCAGACCCTGCAGCTACAG CAAAAAGTCCCACTTTGACTTTGAGGAAGAGGACGTGGACAAACTTGAAATCCGAGTTGACCTCTGGAAC GCCAGCAACCTGAAGTTTGGGGATGAGTTTCTCGGGGAACTGAGGCTTCCTCTCAAGATTCTGCGACACT CGAGCTCTTATGAAGCCTGGTACTTCCTCCAGCCCCGAGACAACGGCAATAAGAGCCTGAAGCCAGATGA CTTGGGGTCTCTGAGGTTAAATGTCGTTTATACAGAAGACCATGTCTTCTCCTCTGAGTACTACAGCCCA CTGCGTGACCTGTTACTGAAGTCTGCAGATGTGGAGCCTGTCTCAGCCTCAGCAGCTCACATCCTGGGTG AGGTGTGCAGAGACAAGCAAGAGGCAGCCATCCCGCTGGTACGGCTCCTGCTGCACTATGGCAGGGTAGT GCCCTTCATCAGTGCCATCGCTAGCGCAGAGGTGAAGAGGACCCAGGACCCCAATACCATCTTCCGAGGA AACTCACTGACATCCAAGTGCATAGATGAGACGATGAAGCTGGCGGGCATGCACTATCTCCATGTGACCC TGAAGCCCACCATTGAGGAGATCTGCCAGAGCCACAAGTCCTGTGAGATCGACCCTGTGAAACTGAAAGA CGGCGAAAACCTCGAGAACAACATGGAGAGCCTGAGGCAGTATGTGGATCGCATCTTCACTGTCATCACC AAGTCCGGGGTGAGCTGCCCCACCGTCATGTGTGACATCTTTTTCTCCTTGCGGGAGGCGGCTGCCAAGC GCTTCCAAGATGACTTGGATGTGAGGTACACGGCCGTGAGCAGCTTCATCTTCCTCAGGTTCTTCGCCCC TGCCATCCTGTCCCCAAACCTTTTCCAGCTGACACCCCACCACACGGATCCACAGACTTCTAGAACCCTG ACACTTATCTCAAAGACGATCCAGACCCTCGGCAGCTTGTCCAAGTCCAAGTCTGCCAGTTTTAAGGAGT CGTACATGGCGACATTCTATGAATTCTTCAATGAGCAGAAGTATGCAGATGCTGTAAAAAATTTTCTGGA TTTGATCTCATCCTCGGGGAGAAGGGACCCCAAGAGCATAGAGCAGCCCATCCTGCTTAAAGAAGGGTTC ATGATCAAGCGGGCCCAGGGAAGGAAACGGTTTGGAATGAAGAATTTCAAGAAGAGGTGGTTTCGCCTGA CGAACCACGAGTTCACCTACCAGAAAAGCAAAGGTGATCAGCCACTCTGCAACATCCCCATCGAGAACAT CTTGGCTGTGGAGAGGCTAGAGGAGGAGTCCTTCCGAATGAAAAACATGTTCCAGGTCATCCAGCCAGAG CGTGCCCTGTACATCCAGGCCAACAACTGTGTGGAGGCCAAGGACTGGATCGACATCCTCACCAAAGTGA GCCAGTGCAACCAGAAGCGGCTCACCGTCTTCCACCCGTCGGCCTACCTGAACGGCCACTGGCTCTGCTG CAGGGCCTCCTCAGACACGGCTGCTGGCTGCACTCCCTGCACTGGTGGGCTCCCGGCCAACATCCAGCTG GACATTGATGGGGACCGTGAAACAGAGCGCATCTACTCTCTCTTTAACCTGTACATGGGCAAGCTGGAAA AGATGCAGGAGGCCTGCGGCAGCAAGTCTGTGTATGACGGCCCTGAGCAGGAGGAGTACTCAACGTTCGT CATCGACGACCCCCAGGAGACGTACAAGACGCTGAAGCAGGTCATCGCTGGGGTGGGGACCCTTGAGCAG GAGCACGCACAGTACAGGAGAGACAAGTTCAAGAAGACGAGATACGGGAGCCAGGAGCACCCTATTGGAG ACAAGAGCTTCCAGAACTACATCCGGCAGCAGTCTGAGATCTCCACCCATTCCATTTAAGGTCACAGCAC CTCCCAGATCAGCGCTGTTGACCCACACCAGGGGGAAAAAGCAGATAAAAGAAGGCAGAAGGCATACATG GAGAGGAAACATGTTCACCCGTTCACACGGGTCCTACCAAGCTGGTGGGGCCACCACGAGTCAAGCATGC TCTTCCCCGGCAGGAACTGTTGCCTGGGCTGTGCACTCCCCTTAGCTGCCAGCACAGGTGGTCTGAGCCA TCCTGGAATGTATGCTCATCTGTCTTTCTACTCCTTCAGTTGTATGGGGATCCAGGGATCTGTTAAATAT TAGGGAAGTATGGACTTTGTCTAAACCTGTTCCCTCGCAGCCCGGTCCCCCTTCACAAAGGCACGCCTGG TTCGTTCATGCCAGGGCGCACCTTCTTCAGTGTGCACTCTTGTCCCTTGCCTGCTGGTCTCTGTGGCTCC GTACACATGTAGCAGTCTGGCCCTAGCCTTAGACGTACACGCAATCCTGCCGTTCGTTCCACGGAATCCC TGTCTGAAACGTGAGAAGCAGTATTCCCTGGCAGCCAGCAGGCAGGCAGCCTTTCTCCTGAGCTTATGTT GGTTTGTTCCTGGCTAAAGGGTCATTTTTGTGGCTGGGATGGACAGGATGGTACAGAAACTCCTTTATCT TAGAGGACATGGGGGTCCTTTTCTTAGAGTCCCTGGAGAGGGAGGGCTGTGAGCCCAGTCTTCTCTGGGT GACCATCACCCCTCAGTTCAAACACAGGTCTGGATGTTGGATAAGGTTTCAAACTAGAATCCACAGGCTT GCTGCCTCGATGAAAGTCTGGGAAAGATTTTTAAAGGCTGTGCATTTTTCAAGCTGTCTTTCTTAATGTT TTTAAAGGACTGTTTTTAAATAGCATTTTGATATAAGGCAGTGTGGAATACCAGCATCTATGTGCATTAG GGGGGTGACCCAGGGAAATCCAAAGGGAACAGTATTTGATTTCTCACAAAGCCAAAGGGTCCTCGGGGAC ATAGCTCTGAGATGGAGCCTGCACCGGCAGCAATAAAGGCCAAACGTGGTATTCCACATGCCTGTGTCTC TGTGAGCCCAACAGGCCACACATGCCAATGTCTGGAACCACTTTTGATTGGGGTGGGAGGGTCCTGAAAC AGACAAGACAGACTGTACCAGCTTAGCCCCCACAGTACTGCTGGAGCCTTATGCCAAACTGCATTTCCAG AGGCTGAGAGAGGGGCACTCTATGGGCAGATGTTGCTGAAGGACACAACCACACATTCTGGTACCCTTTT CTCTTCATTCTTGTTGGGAGGTTTAATCTGTATCTAGAACCCATAGTAGCAGAGGATGGAAACAAAAGTC AGTTTGGACACAGCAGGCCCCTTGTACTGAACTTCTTTTTAACTTGGATTTGTAACTTAATGTTTCTGTT TATAAGATACTAACGATTTGCAATGTATTTTAATGGGCAATTAAAGCAGATGTTTTAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:30102545 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30102545 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11725 same embryo
	EMAGE:11727 same embryo
	EMAGE:11728 same embryo
	EMAGE:11724 same embryo
	EMAGE:11722 same embryo
	EMAGE:11723 same embryo
	EurExpress:euxassay_006677 same experiment
	MGI:4827620 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source