EMAGE mouse gene expression spatial database

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EMAGE:11791

Sybu syntabulin (syntaxin-interacting) ( MGI:2442392)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006982. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006982_01	euxassay_006982_02	euxassay_006982_03	euxassay_006982_04

euxassay_006982_05	euxassay_006982_06	euxassay_006982_07	euxassay_006982_08	euxassay_006982_09

euxassay_006982_10	euxassay_006982_11	euxassay_006982_12	euxassay_006982_13	euxassay_006982_14

euxassay_006982_15	euxassay_006982_16	euxassay_006982_17	euxassay_006982_18	euxassay_006982_19

euxassay_006982_20	euxassay_006982_21	euxassay_006982_22	euxassay_006982_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11791_wholemount_strong.wlz

11791_wholemount_moderate.wlz

11791_wholemount_weak.wlz

11791_wholemount_possible.wlz

11791_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11791_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 10 11 weak expression: see section 12 13 14 15 16 17

diencephalon

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

corpus striatum

strong

regional

strong expression: see section 04 05 06 07 08 18 19 20 21 22

telencephalon mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15

olfactory cortex

strong

regional

strong expression: see section 18

olfactory cortex mantle layer

strong

regional

strong expression: see section 13 17 moderate expression: see section 12 16

olfactory cortex marginal layer

strong

regional

strong expression: see section 13 17 18 moderate expression: see section 12 16

hindbrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 07 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 16 17 18

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

retina

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23

nasal cavity

moderate

regional

moderate expression: see section 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 09 10 11 12

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8401
Entity Detected:	Sybu, syntabulin (syntaxin-interacting) ( MGI:2442392)
Sequence:	sense strand is shown >T8401 CGTAATCCTAGCCATCACAGTTTACAGCATCTAAATACCTTAACCATAGCTTAACAATAACACACATACA ATAAGAGGAGGGTGGATCACTCACACAAGCTCTTACAGGAAAACAGGCAGCTCTCTCCCTGGTAAGAAAA ATATGGGTCTCCACCCCACCACAAAAAAAAATCCCAGGATGCCCCAGAATGCAGTTTCTTTTCTTCATGA CGTGTTGTAAAGCACGTCAGCTGCTCCTTGTTGCTGTGCAGATCACCCAACATCGGGTCACTGAGAGACG GCAGCGCTTGCTCGCACAGCTGATAGGATTAGGAGCTCGACTGCTTTGTCAGCCCTGATTGAGTCCAAGA CAGCCCCGGCAGGCACACGCAGGTGCCTCCTCCTGGACGGCGGCGGCGGCGGCAGCGGCGGCGTGGGGAG CCGGCAGGCAGCAGCGAAATGGGGCCCCTCCGAGAGAGCAAGGAGCAGAGAGTGCAGCATCAGGAGAAAG AGATTTCCAGGAGCCGGATTCCCCGGTTGATTCTGCGGCCCCATCGGCCTCAGCAGCAGCAACAGCAGCA GAACAAGGTTTCCCCAGCCTCCGAGTCTCCCTTTTCGGAGGAAGAAAGTAGAGAGTTCAACCCCAGCAGC TCTGGACGCTCAGCAAGGACGATTAGCAGCAACAGCTTCTGCTCAGATGACACAGGTTGTCCCAGCAGCC AGTCGGTATCGCCTGTGAAGACTCCTTCAGACACAGGGCACAGTCCCATTGGCTTTTGCCCTGGAAGCGA TGAAGACTTTACCAGGAAGAAATGCAGGATTGGAATGGTTGGTGAGGGAAGTATTCAGTCAGCTCGTCAT AAAAAAGAACCCAAGGGAGGCATCATAAAGCCAGGCAGTGAAGCAGATTTTAGCTCCTCGAGCAGCACAG GCAGCATCTCGGCTCCTGAGGTCCACATGTCCACGACAGGAAACAAGAGAGCCTCTTTCTCACGCAACCG AGGCCCTCATGGGCGGAGCAATGGAGCATCATCCCACAAGTCTGGCAGCAGCCCACCATCCCCAAGGGAA AAAGACCTTGTGTCTATGCTGTGCAGGAATCCACTGAGCCCCAGTAACATCCACCCTAGTTACGCCCCTT CTTCTCCAAGTAGCAGCAACTCCGGCTCCTACAAAGGAAGTGATTGTAGTCCAGTCATGAGAAGGTCTGG AAGATACATGTCTTGTGGCGAAAATCACGGTGTCAAACCCCCAAATCCAGAACAGTATTTGACTCCTCTG CAGCAGAAGGAGGTCACAGTGAGGCACCTGAGGACCAAGCTGAAGGAGTCTGAGCGCCGACTCCATGAGA GGGAATCTGAAATCATGGAGCTCAAGTCTCAGCTGGCTCGAATGAGGGAGGACTGGATTGAGGAAGAGTG CCACAGAGTGGAGGCTCAGTTGGCACTCAAAGAAGCCAGAAAAGAGATTAAACAGCTCAAACAGGTCATC GAGACTATGAGGAGCAGCTTGGCTGATAAAGATAAAGGCATTCAGAAGTACTTTGTGGACATAAACATCC AAAACAAGAAACTGGAGTCTCTGCTTCAAAGCATGGAGATGGCACACAATAGTTCCCTGAGGGATGAACT GTGTCTTGACTTTTCCTTTGATTCCCCAGAGAAAAGTTTACCCCTGAGCAGCACATTTGACAAGTTGCCA GATGGGTTATCTCTGGAAGAACAGATAACAGAGGAAGGTGCTGACAGTGAGCTTCTGGTGGGAGACAGCA TGGCTGAAGGCACAGATCTGTTAGATGAGATGGTGACTGCCACCACCACAGAATCCAGTGGCCTGGAGTT TGTTCATTCCACTCCAGGGCCACAAGCCCTCAAGGCTCTCCCCTTGGTGAGCCACGAAGAGGGCATTGCG GTGATGGAGCAAGCCGTGCAGACCGACGTGGTGCCGTTCAGCCCTGCCATCTCAGAGCTCATTCAGAGTG TGCTAAAGCTGCAGGACTACTGTCCCACAAGCTCAGCATCTCCAGATGAATCTGGAGCTGACTCGATGGA AAGCTTCTCAGAATCTATCTCTGCCTTAATGCTTGATTTAACTCCAAGAAGTCCTAACTCGGCCATCCTT CTGTCTCCTGTGGAGATCCCATTCAGCAAGGGGGCTATGGAAGCCCATGCAAATCGCCTCATGAGAGAGC TAGATTTTGCAGCCTACACAGAAGAAAGATTGGACAGTGTCCTCTCACTGTCCCAGGGCAGTGTTGTGAG GCAGTACTGGAGCAGCAATTTCTTGGTGGATCTACTGGCTGTGGCTGCCCCTGTGGTACCCACTGTTCTG TGGGCATTCAGTACTCAGAGAGGGGGTACAGATCCTGTCTACAACATCGGAGCTTTGCTCCGAGGTTGCT GTGTGGTGGCCCTACACTCACTACGCCGCACCGCTTTCCACATGAAAACCTAATTAGTTGTTACTGTGTG CCAATGTATCTGTGTGGCCTGATGCCAGGTAGAGCAACAGCAGGTGATCAGTGGAAGTCTCTATTCTGTC AGTTTTGCTTCTCGCTATTTGATTTGCACTATAGTCAGTTGAAGCCTGTTCACTGTTTAAACCAGAGGTA TTTTCCAAGGCATGGAAACCTGGTTCTGGTAGCTGTCCCACCAGAGTGGTGTAGAAAGCATGCTTATGCC CCTGCCGTGTTGTCTGAGGTGCCCGTTCTTATACTAATGGTTCAGGAAGAGAAAATGCCGTTTGCACTTT CACCACAGCCTTTCTAAGGCTGGCATGTTATCTCCTTGCTTTGCTTTGTGCCGTTTGAAAATGTGTAATT GTTCCAGCATTCCAATGCTCTTGTGCATAGCAGGGGACTGTAACCAAAAATAAAAATGTACTTGTGTAAT TGGTTCAAAGACTTGAATAGCTCTTTATCGTCTTGAGGTTGATAAAGTTTGAGTGTTTAGATTTTTGTTT TTTAACTTAATGTAGCTCCAAAGTCTTATAAGACTCATTTGCTCTTAAACCTGTCAGTTAGTGATACTAT GTAAATTTACAATGTACTAACTTATTTTTTGCTTATTATATATAGTGTTTTTTGGAAATTGTTTGTACCC ACATACTTAAAGCATGAAAATAAAGGTTAGTGGTTCCATTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686153 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686153 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11790 same embryo
	EMAGE:11786 same embryo
	EMAGE:11788 same embryo
	EMAGE:11787 same embryo
	EMAGE:11789 same embryo
	EurExpress:euxassay_006982 same experiment
	MGI:4822765 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source