EMAGE mouse gene expression spatial database

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EMAGE:12035

Bmi1 Bmi1 polycomb ring finger oncogene ( MGI:88174)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008247. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008247_01	euxassay_008247_02	euxassay_008247_03	euxassay_008247_04

euxassay_008247_05	euxassay_008247_06	euxassay_008247_07	euxassay_008247_08	euxassay_008247_09

euxassay_008247_10	euxassay_008247_11	euxassay_008247_12	euxassay_008247_13	euxassay_008247_14

euxassay_008247_15	euxassay_008247_16	euxassay_008247_17	euxassay_008247_18	euxassay_008247_19

euxassay_008247_20	euxassay_008247_21	euxassay_008247_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12035_wholemount_strong.wlz

12035_wholemount_moderate.wlz

12035_wholemount_weak.wlz

12035_wholemount_possible.wlz

12035_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12035_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 15 16

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 05 06 07 08 19 20 21 22

spinal cord

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17

vomeronasal organ

strong

regional

strong expression: see section 11 13 14

esophagus

moderate

regional

moderate expression: see section 10 11

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15

lower jaw molar

strong

regional

strong expression: see section 06 17 18

upper jaw incisor

strong

regional

strong expression: see section 10 11 12 15 16

upper jaw molar

strong

regional

strong expression: see section 06 17 18

renal cortex

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17 18

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09

right lung

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

trachea cartilaginous ring

strong

regional

strong expression: see section 11 12

extraembryonic component

moderate

regional

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9379
Entity Detected:	Bmi1, Bmi1 polycomb ring finger oncogene ( MGI:88174)
Sequence:	sense strand is shown >T9379 AATGCATCGAACAACCAGAATCAAGATCACTGAGCTAAATCCCCACTTAATGTGTGTCCTGTGTGGAGGG TACTTCATTGATGCCACTACCATAATAGAATGTCTACATTCCTTCTGTAAAACATGTATTGTACGTTACT TGGAGACCAGCAAGTATTGTCCTATTTGTGATGTCCAGGTTCACAAAACCAGACCACTCCTGAACATAAG GTCAGATAAAACTCTTCAAGATATTGTATACAAATTAGTCCCAGGGCTTTTCAAAAATGAGATGAAGAGA AGAAGAGATTTTTATGCAGCTCACCCGTCAGCTGATGCTGCCAATGGCTCCAATGAAGACCGAGGAGAAG TTGCAGATGAGGAGAAGAGGATTATAACTGATGATGAGATAATAAGCTTGTCTATTGAGTTCTTTGATCA GAGCAGATTGGATCGGAAAGTAAATAAAGAGAAGCCTAAGGAAGAGGTGAATGATAAAAGGTACTTACGA TGCCCAGCAGCAATGACTGTGATGCACTTGAGAAAGTTTCTCAGGAGTAAAATGGACATACCCAATACTT TCCAGATTGACGTCATGTATGAAGAGGAACCTTTAAAGGATTACTACACGCTAATGGACATTGCCTACAT TTATACCTGGAGAAGAAATGGCCCACTACCTTTGAAATACAGAGTTCGGCCAACTTGCAAAAGAATGAAG ATGAGTCACCAGAGGGATGGACTGACGAATGCTGGAGAGCTGGAAAGTGACTCTGGGAGTGACAAGGCCA ACAGCCCAGCCGGAGGTGTTCCCTCCACCTCTTCCTGTTTGCCCAGTCCCAGCACTCCAGTGCAGTCTCC TCACCCACAGTTCCCTCACATTTCCAGTACCATGAATGGAACCAGCAACAGCCCCAGTGCTAACCACCAA TCTTCCTTTGCCAGTAGACCTCGAAAATCATCACTAAATGGGTCATCAGCAACTTCATCTGGTTAGGACT GTTAAGGAAAAGATTTTTCAACCCCCTGATTTAGTTACCTTCATTCATTACAGCTTTATAGATGCTTAAT ACATGTGACTGTCGTCCAGTTTGCTTCCTTTTGTAGTGACTTTAAATTTGGCCATAAATGATGGACTAGA TGTGATACTTCATATGGATGTTAAGTGGAAAGATTGATTCTTTCTCTAAAGAATTGGATTCTGAGAAGGA TTCTGTGTTAGGAAAGATGTGAAATGATTTCTGTGACCACTGTTTGGATCTGGAAATGTTCTACAGTGGG TAGACATTGGGCCATAGTTTGTTAATCTCAATTAATGCCTACATTACATTCTCTTTGATTGTTCTTGTTA TTATGCTGTTCTGTGAACCTGTAGAAAACAAGTGCTTTTTATCTTGAAATTCAGCAAATGGAAAGAATAA GCATAGAATACTGCATTCTGTGCAGCCACGTCACTGTGAATAACAATTTCTTGCATATTTAGCCATTTTA ATTCCTGTTTGATTTTTACTTCTCTGTTGCTACACAAAATGATCAAAGGAAACTTGTTTTACAATCTGTA TGCCTAAAAAGCGGGTACTACCGTTTATTTTACTGACTTGTTGAAATGATTCACTTTTGTAAGAATCAGA TGGCATTATGCTTGTTGTACAATGCCATATTGGTATATGACATAACAGGAAACAGTATTGTATGATATAT TTATAAATACTATGAAAATATTGTGTTTCATGCATTCAAAAACAGTTGTAAACTTCTCCAAATGGGTTCG ACCTTTGCGGATATCACAGTGCACCCAATGCTGAGGCTTACCACCAGCACAGAATTTGTTTTAATGTTGA TAACCAGTTTCAAATTCTTTTCCACTTAGAAGCAAATGGCAGATCTTCATAGTTCTGGCTTTTCTCCAAT AAGACTTTCAAAATTAATATTGCAGTAAAGAAAAATTTTAACCAAGGCAAATATAGTGTTTAATTTTCCA TTGGTTAAAATAGAATTAATGTTTTTAAATTCTGTTTATCAGTGGAATTCTAAAATAGATTTTAAAGTCA ACCAGCTAATTGCTGAAGTAAAGCATTTTATCTACAATTTTATTTTAGTGTATGTGGCACAAGTGTAATT TCAAATTTAAGAGTTGTTTTCATAGTTAAAGGAATCTGCCTTCACTGTAACGTCTGGAAACAGAAACAAT TTGTTATGATTTCTACAGGGATTTTTAATAGAGCAAACATGTTGAATTTAAAATATGAATGACCCCTCCA AGTCTCAGTTTTGCTTTGGTCGAACTTAGCGTGTGTTTATCAGCCATCAGTTATTTGTGAGGATGTTAAT TCCGTATGAATATTGTTTCATGTTTGTATGGGAAACTGTAGCTAAGCATTTCATTGTCTGCAGTCTGCAG AAGAAGCACAATTGTATTGCTTTGTCTTGCTTGTAGCCATTAAATCATTACTTTTACACACATTGCTGTT AACTTCTGCTTTCTTTAAAGATTTAGTAAATGATGTTTTATGAAGCCACAAGATACATATATTTTTATTT TGACCTAAATTTGTACAGTCCCATTGTGTGTGTGTCATTTCTAATTATAGATGTAAAATTAAATTTCATT TTTAATTGGAAAATTCAATAAAAAGATATTCATTTAGAAAATACTATGCTCTTTAATTAAAATTTTGCTA TGAAAAGCACAGTGTGCAGAAGTTTTGGGAACCCTGTAGTGGATTGTAAGAGCTAAAACCTAAGGAAAAT ACTTTGTTGTCTTCAAATGATGTTGTTAGTAAATGCTTTGTTGTATATTGCTCGAAAGCCCAATGAAAGA AGTATCTGCTTATTTACAATCTTTGAAGCAAAAGTTACCAATGTTTGCCAATTTTGGTGAATTTAACTCA TTTTTCTAGACTTTTCTCGAGGTTTTCATGGTGTTACCTAAGACAAAAGACATCTCACCCTCTATGATGG ACTTACTTCTGAGAGTGCGTTTGAGGCACTTATGGCTTACTAAGCAGTGTGTCACCATACTTGAAAACAC TTCCATTTATTGTATCTGGGATGAGGCTTTTTACCCTTACTCAATTTGAAAATTGCTTAAGCTTAAATGA TATTTCAGTCAAAATTTGTCTTTTAATAAAACAACAGAAAGATGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854104 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854104 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12033 same embryo
	EMAGE:12037 same embryo
	EMAGE:12036 same embryo
	EMAGE:12034 same embryo
	EMAGE:12038 same embryo
	EurExpress:euxassay_008247 same experiment
	MGI:4823482 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source