EMAGE mouse gene expression spatial database

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EMAGE:12037

Wwp2 WW domain containing E3 ubiquitin protein ligase 2 ( MGI:1914144)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008277. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008277_01	euxassay_008277_02	euxassay_008277_03	euxassay_008277_04

euxassay_008277_05	euxassay_008277_06	euxassay_008277_07	euxassay_008277_08	euxassay_008277_09

euxassay_008277_10	euxassay_008277_11	euxassay_008277_12	euxassay_008277_13	euxassay_008277_14

euxassay_008277_15	euxassay_008277_16	euxassay_008277_17	euxassay_008277_18	euxassay_008277_19

euxassay_008277_20	euxassay_008277_21	euxassay_008277_22	euxassay_008277_23	euxassay_008277_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12037_wholemount_strong.wlz

12037_wholemount_moderate.wlz

12037_wholemount_weak.wlz

12037_wholemount_possible.wlz

12037_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12037_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23 24

forelimb digit 1 metacarpal

strong

regional

strong expression: see section 01 24

forelimb digit 1 phalanx

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

forelimb digit 2 metacarpal

strong

regional

strong expression: see section 01

forelimb digit 2 phalanx

strong

regional

strong expression: see section 01 02 03 04 21 22 23 24

forelimb digit 3 phalanx

strong

regional

strong expression: see section 02 03

hindlimb digit 1 metatarsal

strong

regional

strong expression: see section 21

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 02 19 20 21

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 01 21 22

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 01 19 20 21 22

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 01 21 22

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 01 02 18 19 20 21 22

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 01 21

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 01 02 03 18 19 20 21

hindlimb digit 5 metatarsal

strong

regional

strong expression: see section 21

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 01 02 03 04 18 19 20 21

tarsus

strong

regional

strong expression: see section 02 03 21 22

fibula

strong

regional

strong expression: see section 23 24

tibia

strong

regional

strong expression: see section 23 24

femur

strong

regional

strong expression: see section 01 02 04 05 06 07 22 23 24

otic capsule

strong

regional

strong expression: see section 05 06 07 08 15 16 17

heart valve

strong

regional

strong expression: see section 09 10 moderate expression: see section 11 12 13

hyoid

strong

regional

strong expression: see section 08 09 10 11 12 14

mandible

strong

regional

strong expression: see section 03 04 05 06 07 13 14 15 16 21 23 24 moderate expression: see section 08 09 10 11 12 17 18 19 20 22

meckel's cartilage

strong

regional

strong expression: see section 04 05 06 07 08 12 13 14 15 16 20 21 moderate expression: see section 09 10 11 17 18 19

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 13 14 15 16 21 23 moderate expression: see section 08 09 10 11 12 17 18 19 20 22

arytenoid cartilage

strong

regional

strong expression: see section 10 11 12 13

cricoid cartilage

strong

regional

strong expression: see section 10 11 12 13

thyroid cartilage

strong

regional

strong expression: see section 10 11 12 13 14

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22

trachea cartilaginous ring

strong

regional

strong expression: see section 11 12 13

axial skeleton

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

basioccipital bone

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basisphenoid bone

strong

regional

strong expression: see section 01 02 03 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 04 05 18 19 20 21 22 23 24

vault of skull

strong

regional

strong expression: see section 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23

clavicle

strong

regional

strong expression: see section 06 07 08 09 15 16 17 18 19

scapula

strong

regional

strong expression: see section 01 02 03 04 20 21 22 23

sternum

strong

regional

strong expression: see section 11 12 13 14 15

pelvic girdle skeleton

strong

regional

strong expression: see section 08 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9161
Entity Detected:	Wwp2, WW domain containing E3 ubiquitin protein ligase 2 ( MGI:1914144)
Sequence:	sense strand is shown >T9161 CGGAAGTGGAGCGGAGTTAGGCGCCGCTGGTGTGGCCGCGGCAGTTCCATTGTGATGACATGGCATCTGC CAGCTCCAGCCGGGCAGGAGTGGCCCTGCCTTTTGAGAAGTCCCAGCTTACCCTGAAAGTGGTGTCAGCA AAGCCCAAGGTGCACAACCGCCAGCCCAGAATCAACTCCTACGTGGAGGTGGCAGTGGATGGACTCCCCA GCGAGACCAAGAAGACGGGGAAGCGCATCGGGAGCTCTGAACTGCTCTGGAATGAAATCATCGTTCTGAA TGTCACAGCCCAGAGTCATTTGGATCTGAAGGTCTGGAGCTGTCATACCTTGAGGAATGAACTACTGGGC ACTGCCTCTGTCAACCTCTCCAATGTCCTGAAGAACAATGGCGGCAAAATGGAGAACACACAACTGACCC TGAACCTGCAGACAGAGAACAAAGGCAGTGTTGTCTCGGGAGGAGAGCTGACAATTTTCCTGGATGGGCC AACTGTTGATCTGGGAAGTGTGCCTAATGGCAGTGCAGTGACAGACGGATCACAGCCACCTTCAAGAGAA TCCAGTGGGACTGCTATAGCTCCAGAGACCCGGCACCAGCCCCCCAGTACAAACTGCTTTGGTGGCAGAT CCCGGACGCACAGACACTCAGGTGGCTCAGCCAGGACAGCCACAGCAGCCAGTGAACAAAGCCCTGGCGC TAGGAACCGCCACCGCCAGCCTGTGAAGAACTCTAGCAGCAGTGGCTTAGCCAATGGCACAGTGAATGAG GAACCTACTCCAGCCAGTGAACCTGAAGAATCGTCGGTTGTTGGTGTAACATCCCTGCCTGCAGCAGCCT TGAGTGTGTCCTCAAATCCCAACACAACATCTCTCCCTGCACAGTCCACACCAGCAGAGGGAGAGGAGGC CAGCACTTCCGGGACACAGCAGCTCCCTGCTGCCGCCCAGGCCCCTGATGCTCTTCCTGCTGGATGGGAA CAGAGAGAGCTGCCCAATGGGCGTGTCTATTATGTTGATCACAACACCAAGACCACCACCTGGGAGCGGC CTCTTCCTCCAGGGTGGGAAAAGCGCACGGACCCCCGAGGGAGGTTTTACTACGTGGACCACAACACCCG GACAACCACCTGGCAGCGCCCAACTGCTGAGTACGTGCGCAACTATGAGCAGTGGCAGTCCCAGCGGAAC CAGCTGCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTCTACCAGTCTTCGAGTGCTTCGACTGACC ATGATCCCTTGGGCCCCCTCCCACCTGGCTGGGAGAAGAGGCAGGACAATGGACGGGTGTATTATGTCAA CCACAACACTCGCACTACCCAGTGGGAGGACCCTCGGACCCAGGGGATGATACAGGAGCCAGCCCTGCCC CCAGGGTGGGAGATGAAATACACCAGCGAGGGCGTGCGGTACTTTGTGGACCACAATACCCGCACCACTA CCTTTAAGGATCCTCGCCCAGGGTTCGAGTCAGGGACAAAGCAAGGCTCACCTGGTGCCTATGACCGAAG TTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGCCACTCAAATGCTCTACCCAGCCATGTGAAGATC AGCGTTTCCAGGCAGACACTCTTTGAGGATTCTTTCCAACAGATTATGAACATGAAACCTTACGACCTGC GCCGCCGGCTCTACATCATCATGCGTGGTGAGGAGGGCCTGGACTACGGCGGCATCGCCAGAGAGTGGTT TTTCCTCCTGTCCCATGAGGTGCTCAACCCTATGTACTGTTTGTTTGAATATGCTGGGAAGAACAATTAC TGCCTGCAGATCAACCCGGCCTCTTCCATCAACCCTGACCACCTCACCTACTTCCGCTTTATCGGCAGAT TCATCGCCATGGCTCTGTACCATGGGAAGTTCATCGACACAGGCTTCACTCTCCCTTTCTACAAGCGGAT GCTCAACAAGAGACCGACTCTGAAGGACCTGGAGTCTATTGACCCTGAGTTTTACAACTCCATTGTCTGG ATCAAAGAGAACAACCTGGAAGAGTGTGGTCTGGAGCTGTTTTTCATCCAGGACATGGAGATTCTGGGCA AGGTGACAACCCATGAACTGAAGGAGGGCGGTGAGAACATCCGAGTTACCGAGGAGAACAAGGAGGAGTA TATCATGCTGCTGACTGACTGGCGATTCACCCGAGGCGTGGAAGAGCAGACCAAAGCTTTCCTGGATGGC TTCAATGAGGTTGCCCCTCTGGAGTGGTTGAGATATTTTGATGAGAAAGAGCTGGAGCTCATGCTCTGCG GCATGCAGGAGATAGACATGAGCGACTGGCAGAAGAACGCCATCTATCGGCACTACACCAAGAGCAGCAA GCAGATCCAGTGGTTCTGGCAGGTTGTCAAGGAGATGGACAATGAGAAGAGGATCCGGCTACTGCAGTTT GTCACGGGAACCTGCCGTCTGCCTGTTGGGGGATTTGCTGAGCTCATCGGGAGCAATGGCCCGCAGAAGT TCTGCATCGACAGAGTTGGCAAGGAAACCTGGCTGCCCAGGAGCCATACGTGCTTCAACCGTCTGGACCT GCCTCCCTATAAGAGCTACGAGCAGCTGAAAGAGAAGCTGCTGTACGCCATCGAGGAGACTGAGGGGTTC GGACAGGAGTAGCTGAGGCTGCTGTCTCACACCCTCTAGCACATGTAGTCCTGAGTCCTTCCTGCCGGAG ATGCCACCGGTTGCACAGCCCTCAAGAGGCCCCTTTGGATAGTGGCCCTGCACGAGACTGTGCCTTGATC ATTTCGGTGACTGAAGAGTCTGACCCCAGAGGCCTTGGGGACCCTTTCTTCTCTCTGATGATGGCAGTCT GGAATAAAGCACTGTCACCTTTGACTCTATTACCTGTTGCAAAGCCAGAGGGCTGCCCTTCTCTGCAAAA CTGCCCCTTCCACTAGGACTGAGTCTGGGTGTGTGTGAGAGTGTGAGGGAACATGCCCTGCCTTAGAGGG CTGCCACTGAAGCTGGGCCTCATATGTTCCATGAGGAGCTAGGCCTCGTGGAATGGCTGGCTGACTAAAC CCAGAAAGCCAAGGCTCTGCCAGTGGAAGAGACTTCACTTTTGTAGACCTTCCCTGAAGTCCATGGAACA GACCTAAGGGAACAACTGAGCATCAAAACCGGCTTTGCTTTACTTCCTGGCAGGCTGCAGCCAAGGGGAC TGAGCAGCAGGCACCCAATGCCACCAGCTCGTCTCTTCCTAGCAAGCAAAGGGTTAAAGAAGCCACCACC TGGGTGTCAGTGGGAAGGAGACAGCAGAGCTCAGGCTTTGCCCTTTGACAGAAACCTAAAGACACATCCA CATCCATGCATAGGGCTGTTGCTTGTTGTGTTGTAGGCATGCTCTTCTCACCTTCTGTCAAGGACCTGGT GCCCTGGGTGGTGGAGGGCTCAGCCCTCTTGATTCAGTAGCTTCCCAAGCAGCTGCTCCTCTCAGGAGCC TGCTTAACACTTGCGCCAGCTCAGAGGCCTGCCTCTATGGTCCAGTGCATCCCCGCTGTGCTCAGATGCT GACGAGACTCCTTTGCTAGACTCCCCTAGTTAGGGCCTCTGCAGCTTATGGTGGCTTAAAATGGAGAGAG CATCTTGCCTGGCCGAGTGTTTGTGCTATGTGAGCATGAGGCTTTATTTGACTGTGGAAGAAGACACTAA GAGCCAGCAGAAGGGCCTGAGCTGCCTCATTAGCCTGGCTTCATTTGGGGAGCTCCTGACAGTGTCCCGA GTAGGTGCCCAGCCCCTCTTGGGTACCAGAGTGGTTATAGCGACACACTCCTCATCCAGGCTTCCCAAGA TACACTAGCTCTGGGACACCCCAGGCATGCTCTCAAACTAGTCATGGGGCCTTCTGAGGACAGTTCCATC CTACCTTTGTCCTTCCCTGGTACAGCCTGTGACCCTGGCACACTGCACATGCTGATCTGAGTGGCTCTGC TCACTGTCGCACACCCCTTCCTCTCCCATGCAGTCACCTCAGGACTCCCGACATACATCACAAAGGCTGC TTCATGGCTGGGTGTGGGACCAGATGCCAGCAAGGATGGAAGCATCAGGCTCACAGGTGTGTGTGTGTGT GTGCACGCGTTCGCACATGCGTGAGTGTGTAGCAGAAGTGTGTACAGAGAGGCCCGTGCTAGCTAGCACC AAGTTGGTTGACAGATTGTTTTGTGATGCTTGCCCTGCCTCGGTATTGCCAGTTTCTTGTGCAATAAAGA ATCAGCAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827381 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827381 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12035 same embryo
	EMAGE:12033 same embryo
	EMAGE:12036 same embryo
	EMAGE:12034 same embryo
	EMAGE:12038 same embryo
	EurExpress:euxassay_008277 same experiment
	MGI:4829234 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source