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EMAGE:12067

Kank1 KN motif and ankyrin repeat domains 1 ( MGI:2147707)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067
"Pseudo-wholemount" of euxassay_008605. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008605_01 euxassay_008605_02 euxassay_008605_03 euxassay_008605_04
EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067
euxassay_008605_05 euxassay_008605_06 euxassay_008605_07 euxassay_008605_08 euxassay_008605_09
EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067
euxassay_008605_10 euxassay_008605_11 euxassay_008605_12 euxassay_008605_13 euxassay_008605_14
EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067
euxassay_008605_15 euxassay_008605_16 euxassay_008605_17 euxassay_008605_18 euxassay_008605_19
EMAGE:12067 EMAGE:12067 EMAGE:12067 EMAGE:12067
euxassay_008605_20 euxassay_008605_21 euxassay_008605_22 euxassay_008605_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12067Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12067_wholemount_strong.wlz
12067_wholemount_moderate.wlz
12067_wholemount_weak.wlz
12067_wholemount_possible.wlz
12067_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12067_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23
forearm mesenchyme
strong strong
regionalstrong expression: see section 01 02 03
upper arm mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 22 23
hand
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 22 23
foot
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 20 21 22 23
lower leg mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 23
upper leg mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 20 21 22 23
mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 17 18 moderate expression: see section 19 20
brain
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23
diencephalon floor plate
strong strong
regionalstrong expression: see section 11 12
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 11 12 13
diencephalon roof plate
strong strong
regionalstrong expression: see section 12 13
choroid invagination
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 14 15 16 17 18 19
medulla oblongata floor plate
strong strong
regionalstrong expression: see section 11 12
metencephalon floor plate
strong strong
regionalstrong expression: see section 11 12
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain floor plate
strong strong
regionalstrong expression: see section 11 12
midbrain ventricular layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16
spinal cord
moderate moderate
single cellmoderate expression: see section 10 11 12 13 14 15 16 17 18
alar columns
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18
naris
strong strong
regionalstrong expression: see section 11 12 13 14 15 16
anterior naris
strong strong
regionalstrong expression: see section 17
nasal septum
strong strong
regionalstrong expression: see section 11 12
viscerocranium
strong strong
regionalExpression in the turbinate bone.
aorta
strong strong
regionalstrong expression: see section 11 12 13 14
trabeculae carneae
strong strong
regionalstrong expression: see section 08 09 10 12 13 14 15 16 17
heart valve
strong strong
regionalstrong expression: see section 11 12 13 14 15
tongue muscle
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17
stomach
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10
mandible
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16 17
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 19 20
maxilla
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 19 20
renal cortex
strong strong
regionalstrong expression: see section 09 10 11 12 18 19 20 21 22 23
arytenoid cartilage
strong strong
regionalstrong expression: see section 12 13 14 15
cricoid cartilage
strong strong
regionalstrong expression: see section 12 13 14
thyroid cartilage
strong strong
regionalstrong expression: see section 11 12 13 14 15
trachea cartilaginous ring
strong strong
regionalstrong expression: see section 13 14 15
axial skeleton
strong strong
regionalstrong expression: see section 12 13 14 15 16
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
basisphenoid bone
strong strong
regionalstrong expression: see section 11 12 13 14 15
temporal bone petrous part
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 21 22 23
sternum
strong strong
regionalstrong expression: see section 14
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 11 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9409
Entity Detected:Kank1, KN motif and ankyrin repeat domains 1 ( MGI:2147707)
Sequence:sense strand is shown

>T9409
CTTCCACTGAGTCTCTGTCATCCTCCAACAGTGATGACAGCAAGCAGTGTCCCAGCTTCCTGCTAGCCAG
AAGCCACGTTACTTCAACCCCAATCCCACGGCCACCTGCCCCGCTAGAGACCTCACCCACTTTTGCTGTG
TCAGAAAACCGACAGCTGCTGCCCCCTCCCTCACCACAACTCCCCAGGCACAACCTCCATGTCACCAAGA
CGTTGATGGAGACCCGGAGAAGACTGGAACAGGAGCGAGTCACCATGCAGATGGCACCGGGTGACTTCAG
GAGGCCCAGGCTGGCCAGTTTTGGAGGCATGGGCTCCACAAGCTCCCTCCCGTCCTTTGTGGGGTCTGCT
AACCACAGTTCTGTAATACACCAGCTTCAGAATGGCTACCAAGGCAATGGGGATTATAACAGCTACGTCC
CAGCAGCTCCTACCACGTCTTCCATGGGAAGCTCCGTCCGGCACAGCCCATTGAGTTCAGGGATCTCCAC
CCCAGTGACCAACGTGAGCCCCATGCACCTGCAGCACATCCGAGAGCAGATGGCCATCGCCTTAAAACGC
CTGAAGGAGCTTGAGGAGCAGGTGAGGACCATCCCTGTGCTCCAGGTCAAGATCTCCGTCTTGCAAGAAG
AGAAAAGGCAGTTGGCCTCGCAGCTGAAAAGCCAGAGGGCCTCGTCCCAGAACGAAGCGTGCGGTGTGAG
GAAACGCTCCTACAGTGCGGGCAACGCCTCCCAGCTGGAACTGCTTGCCCGAGCCCGAAGAGGCGGCGGG
GAATTGTACATCGACTACGAGGAGGAAGAGATGGAGAGCGTAGAGCAGAGCACGCAGCGGATCCAGGAGT
TCCGACAGCTCACGGCCGACATGCAGGCGCTGGAGCGGAAGATTCAGGATAGCAGCTGCGAGGTGGCATC
AGAGCTCAGGGAGAATGGGCAGTGCCCGTCTCGGGAGTGCAAGTCTGTGGCCGTGGGTAGTGATGAGAAC
ATGAACGATGTCGTTGTATACCACAGAGACTTAAGGCCCTGCAAGGATACGGCTGTGGGGACGGTCACCG
AGACGAGGAATGTTGGCATCAGCGTGACAGAAGCTATGCTTGGGGTTATCACTGAAGCTGACAAAGAGAT
TGAGCTGCAGCAGCAGACCATAGAGGCCTTGAAGGAGAAGATTTACCGCCTAGAAGTACAGCTTAAAGAA
ACCACCCATGACCGCGAGATGACTAAGCTCAAGCAAGAACTACAGGCTGCCGGATCCAGGAAGAAAGTTG
ACAAGGCCACAATGGCCCAGCCACTTGTCTTCAGCAAGTTGGTGGAGGCACTGGTGCCAACCAGAGATCA
AATGGTTGGCAGTCATGTGGACACAAGGGAGTCATGTGTTGGGACCTCTGTGCAAACTAGTAGCGTAGGC
ACCTCCTGCCATCCTGACTGCAAGAACCAAGTCGTGGGATCCGAGCTGCCCATGAATTGGTGGGTCGTGA
AGGAGAGGGTGGCAATGCATGACCAATGCGTAGGGAGGTCTGTTGAGACTTGTGACCGGAGTGTGGGTGT
GGAAGTCAGTGTCTGTGAAACAGGCAGCAACACAGAGGCTTCTGGGAGCGACCTGACACTCCTTAAGACA
AACTTGAACCTCAAAGACGTGCGGTCCATCGGCTGTGGAGATTGTTCCGTGGATGTGATTGTCTGCTTCC
CCAAGGAGTGCACCTCCCGAAGCATGAACACGGAGGCTGTAGGCCAGGAGGAAGCAGCTGTCATGGCGGT
GCCCCATACCACAGACCAGCACACCAGCACGAATCTGGAGCGGGTGGACCAGTGCACTAACACAGAGGCA
GCCACCCTGGTGGAGTCCTGCACCAACACTCTCCTCAGCACTATGGACAAGCAGACCAGCACCCAGACTG
TGGAGATGCGAACGGTGGCCATCGGAGAAGGCCGCGTCAGAGACATCAACCCCTCCACTAAGACTCGGTC
CGTTGGTGTCGGGACAGTGCTGTCTGGCAATTCTGAGTTTGACAGGCCATGTGCTGTGAAGACCAAAGAG
TCGGGTGTGGGACAGATAAATATTCAGGACAACTATCTAGTTGGTCTCAAAATGCGGACCATAGCGTGCG
GGCCTCCACAGTTGACTGTGGGGCTGATGGGCAGCAGGAGGAGCGTGGGTGTTGGGAACGAGCCCGTAGG
AGAGCTCCCGGAGGAGTCTCCTCAGCCTCGGGTGGCGTCTGGAATGGTGACTGGCTTGGATCACTACATT
GAGCGCGTGCAGAGGCTGCTGGCAGAGCAACAGACGTTGCTGGCTGAGAACTACAGTGAGCTGGCGGAGG
CTTTTGGGGAGCCTCATTCACAGATTGGCTCCCTCAACTCGCAGCTCATCAGCACCCTGTCGTCCATCAA
TTCCGTCATGAAGTCTGCAAGCACCGAGGAGCTCAGGAACTCTGACTTCCAGAAAGCCAGTCTGGGTAAA
ACTACAGGAAATCACTTGGAATATACCTGCAAGTGTGGAGGCCTTCGGTCAGGAGGACTGTTAAATGTAC
AACCATCCCAGCCTGAGGTGGAGGCGGAGACTGCAGAAGGCAAGCACAGCCGGGGCCATGAGCAGTTCCC
CATGCAAGGAAGTACCCTGCCCCCGGTGAACCTGACAGATGACCAGATAGCCACTGGCCTCTACGTATGT
CCAAATAATGAAAACACACTGAAGTCCATCATGAAGAAAAGTGATGGGAATAAAGATTCAAATGGAGCCA
AAAAGAATCTTCAGTTCATTGGCATTAACGGCGGGTATGAGACAACTTCAAGTGACGAATCCAGCTCGGA
TGGAAGCTCTTCTTCCGAGTCAGATGACGAGTGTGACACCATTGGGTACCCTCCCGAGGAAGAGGAGGAG
GAGGAAGAGAAGGACCACGACACTCGGGGAATGGCGGAAGGGCACCACGCAGTTAATATTGAAGGTTTCA
AGTCTGCCAGGGTGGAAGATGAAGTGCAGGTTCCAGAATGTGAGCCTGAGAAGGAGGAAATCAGAGAGAG
GTATGAACTAAGTGAAAAGATGTTGTCAGCCTGCAACTTACTGAAATACAACATAAAGGATCCCAAAGCT
TTGGCCAGCAAAGATATGAGGATCTGTCTGAACACCCTCCAGCACGATTGGTTCCGAGTGTCCAGTCAGA
AGTCCGCCGTGCCAGCCATGGTGGGCGACTACATCGCCGCGTTCGAGGCTGTCTCCCCGGACGTGCTCCG
GTACATCATCAACATGGCGGACGGCAATGGCAACACTGCCCTCCACTACAGCGTGTCCCACTCCAACTTC
CAGATCGTCAAGCTGCTTCTGGATGCAGACGTGTGCAATGTGGATCACCAGAACAAGGCAGGGTACACAC
CCATCATGCTGGCAGCCCTCGCGGCCGTGGAGGCAGAGAAGGACATGCAGGTCGTAGAAGAACTCTTCAG
CTGTGGAGACGTGAACGCCAAGGCCAGTCAGGCGGGACAGACGGCCCTCATGCTAGCTGTCAGTCACGGG
CGGATAGACATGGTGAAGGGCCTGCTGGCCTGTGGAGCTGATGTCAATATCCAGGACGACGAGGGCTCCA
CCGCCCTGATGTGTGCCAGTGAACACGGGCATGTGGAGATTGTGAAGCTGCTGCTGGCCCAGCCAGGCTG
CAATGGCCACCTGGAAGACAATGATGGCAGCACCGCTCTCTCCATAGCCCTGGAAGCTGGACACAAGGAC
ATTGCCGTTCTTCTGTACGCCCACCTCAACTTCTCGAAAGCCCAGTCCCCGAGCACCCCGAGGCTTGGCA
GAAAGACATCTCCTGGTCCCACTCACCGAGGTTCTTTTGACTGATTATGTAAGCATGGCCCTTGTGTGTA
CCACCACGAAGCTGCTAGTTATTCCTGTTGGGGTGACAGATACTGAATGTAAATGGACCACACCAAGCTG
ACCAGCAGACAGAAGAGTCAAGGAGCGGCTGAAGGCTGATGCTTCTGCAGGGAAAGCCGAGCTGTTGGGA
AATGGCCTCCTCTCTGGCCCTCTGCTCTAGTAGAGCTTGGACCCAGCTCCGTGGCTGCTGAACCGGCTCT
GTTATTTGCAGTGGTAGGAAATGGTAGACTGGCCTGACCAGGCCATTTCTACCATATAAGCAAATGTCAG
GTTCTCTATGACATTGTAGTTTTGACCTTTTCCACAAGTGCTTAGTAACTTATGAAATGTGGAGCCGTTG
GGAAGTCCTTCCAGTATTCCATTCCAGGGTGCTTCTTGCTTCTGTTCACTTCTCTTTTGTCTTTTAAAAT
AAGAACCTTGGGGGAGAGGACTTTGATCGTCAGCAACTTGCCCCCCCCCCACGCTGATAATTTCCCGCTT
AAAAATAGAAGATGGAACTCTCAAACCATATGTATAAACTTACAAGAAATATTTCAATTGGTTTATCGAG
GTGGATGTATTATATGGCTTTTTATATAAAAGACTTCTATATGAAATTGACAACAGTATTTTCAACTTTT
ATATTCCACAGTACAGTCATAAAGAGCTACATGCAATGTTACGTTTTGAGCATCTGCCCAACCATGTCTA
TATCTGGTGATAAAGGTTTGAATTAGGTTAGAGGACTGGGTGCCAGATTTGGGTCTATGGAGGCAGAGTG
TAGTTCTCTGTGTATTTCTATCATACGCCACGTTTACTTTGGTCCTGTATATGTATTTAAATGTTTGAAG
TGCCTTAGACTCTTGCCATATTTTCAAAATAAAATTTCATTAAGCTCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30535512 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30535512 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12070 same embryo
 EMAGE:12071 same embryo
 EMAGE:12069 same embryo
 EMAGE:12068 same embryo
 EMAGE:12072 same embryo
 EurExpress:euxassay_008605 same experiment
 MGI:4825686 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS