EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12112

Cfh complement component factor h ( MGI:88385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008425. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008425_01	euxassay_008425_02	euxassay_008425_03	euxassay_008425_04

euxassay_008425_05	euxassay_008425_06	euxassay_008425_07	euxassay_008425_08	euxassay_008425_09

euxassay_008425_10	euxassay_008425_11	euxassay_008425_12	euxassay_008425_13	euxassay_008425_14

euxassay_008425_15	euxassay_008425_16	euxassay_008425_17	euxassay_008425_18	euxassay_008425_19

euxassay_008425_20	euxassay_008425_21	euxassay_008425_22	euxassay_008425_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12112_wholemount_strong.wlz

12112_wholemount_moderate.wlz

12112_wholemount_weak.wlz

12112_wholemount_possible.wlz

12112_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12112_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23

humerus

strong

regional

strong expression: see section 01 02 03 04

fibula

strong

regional

strong expression: see section 01 02

tibia

strong

regional

strong expression: see section 01 02

femur

strong

regional

strong expression: see section 03 04 05 06 07 21 22 23

mesenchyme

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

adrenal cortex

strong

regional

strong expression: see section 08 09 10 11 17 18 19

adrenal medulla

strong

single cell

strong expression: see section 09 10 11 17 18 19

thymus primordium

strong

single cell

strong expression: see section 12 13 14 15 16

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate mantle layer

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

hindbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

rest of cerebellum mantle layer

strong

single cell

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

single cell

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord mantle layer

strong

single cell

strong expression: see section 11 12 13 14 15 16 17

spinal cord meninges

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

trabeculae carneae

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

mandible

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23

bladder

strong

regional

strong expression: see section 12 13 14 15 16

renal medullary interstitium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 17 18 19 20 21 22

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23

clavicle

strong

regional

strong expression: see section 05 06 07 08 09 10 18 19 20 21 22 23

scapula

strong

regional

strong expression: see section 04 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9297
Entity Detected:	Cfh, complement component factor h ( MGI:88385)
Sequence:	sense strand is shown >T9297 CTGGATGGTACAGCACAGATTTCTCTTGGAGTCAGTTGGTCCCAGAAAGATCCAAATTATGAGACTGTCA GCAAGAATTATTTGGCTTATATTATGGACTGTTTGTGCAGCAGAAGATTGTAAAGGTCCTCCTCCAAGAG AAAATTCAGAAATTCTCTCAGGCTCGTGGTCAGAACAACTATATCCAGAAGGCACCCAGGCTACCTACAA ATGCCGCCCTGGATACCGAACACTTGGCACTATTGTAAAAGTATGCAAGAATGGAAAATGGGTGGCGTCT AACCCATCCAGGATATGTCGGAAAAAGCCTTGTGGGCATCCCGGAGACACACCCTTTGGGTCCTTTAGGC TGGCAGTTGGATCTCAATTTGAGTTTGGTGCAAAGGTTGTTTATACCTGTGATGATGGGTATCAACTATT AGGTGAAATTGATTACCGTGAATGTGGTGCAGATGGGTGGATCAATGATATTCCACTATGTGAAGTTGTG AAGTGTCTACCTGTGACAGAACTCGAGAATGGAAGAATTGTGAGTGGTGCAGCAGAAACAGACCAGGAAT ACTATTTTGGACAGGTGGTGCGGTTTGAATGCAATTCAGGCTTCAAGATTGAAGGACATAAGGAAATTCA TTGCTCAGAAAATGGCCTTTGGAGCAATGAAAAGCCACGATGTGTGGAAATTCTCTGCACACCACCGCGA GTGGAAAATGGAGATGGTATAAATGTGAAACCAGTTTACAAGGAGAATGAAAGATACCACTATAAGTGTA AGCATGGTTATGTGCCCAAAGAAAGAGGGGATGCCGTCTGCACAGGCTCTGGATGGAGTTCTCAGCCTTT CTGTGAAGAAAAGAGATGCTCACCTCCTTATATTCTAAATGGTATCTACACACCTCACAGGATTATACAC AGAAGTGATGATGAAATCAGATATGAATGTAATTATGGCTTCTATCCTGTAACTGGATCAACTGTTTCAA AGTGTACACCCACTGGCTGGATCCCTGTTCCAAGATGTACCTTGAAACCATGTGAATTTCCACAATTCAA ATATGGACGTCTGTATTATGAAGAGAGCCTGAGACCCAACTTCCCAGTATCTATAGGAAATAAGTACAGC TATAAGTGTGACAACGGGTTTTCACCACCTTCTGGGTATTCCTGGGACTACCTTCGTTGCACAGCACAAG GGTGGGAGCCTGAAGTCCCATGCGTCAGGAAATGTGTTTTCCATTATGTGGAGAATGGAGACTCTGCATA CTGGGAAAAAGTATATGTGCAGGGTCAGTCTTTAAAAGTCCAGTGTTACAATGGCTATAGTCTTCAAAAT GGTCAAGACACAATGACATGTACAGAGAATGGCTGGTCCCCTCCTCCCAAATGCATCCGTATCAAGACAT GTTCAGCATCAGATATACACATTGACAATGGATTTCTTTCTGAATCTTCTTCTATATATGCTCTAAATAG AGAAACATCCTATAGATGTAAGCAGGGATATGTGACAAATACTGGAGAAATATCAGGATCAATAACTTGC CTTCAAAATGGATGGTCACCTCAACCCTCATGCATTAAGTCTTGTGATATGCCTGTATTTGAGAATTCTA TAACTAAGAATACTAGGACATGGTTTAAGCTCAATGACAAATTAGACTATGAATGTCTCGTTGGATTTGA AAATGAATATAAACATACCAAAGGCTCTATAACATGTACTTATTATGGATGGTCTGATACACCCTCATGT TATGAAAGAGAATGCAGTGTTCCCACTCTAGACCGAAAACTAGTCGTTTCCCCCAGAAAAGAAAAATACA GAGTTGGAGATTTGTTGGAATTCTCCTGCCATTCAGGACACAGAGTTGGGCCAGATTCAGTGCAATGCTA CCACTTTGGATGGTCTCCTGGTTTCCCTACATGTAAAGGTCAAGTAGCATCATGTGCACCACCTCTTGAA ATTCTTAATGGGGAAATTAATGGAGCAAAAAAAGTTGAATACAGCCATGGTGAAGTGGTGAAATATGATT GCAAACCTAGATTCCTACTGAAGGGACCCAATAAAATCCAGTGTGTTGATGGGAATTGGACAACCTTGCC TGTATGTATTGAGGAGGAGAGAACATGTGGAGACATTCCTGAACTTGAACATGGCTCTGCCAAGTGTTCT GTTCCTCCCTACCACCATGGAGATTCAGTGGAGTTCATTTGTGAAGAAAACTTCACAATGATTGGACATG GGTCAGTTTCTTGCATTAGTGGAAAATGGACCCAGCTTCCTAAATGTGTTGCAACAGACCAACTGGAGAA GTGTAGAGTGCTGAAGTCAACTGGCATAGAAGCAATAAAACCAAAATTGACTGAATTTACGCATAACTCC ACCATGGATTACAAATGTAGAGACAAGCAGGAGTACGAACGCTCAATCTGTATCAATGGAAAATGGGATC CTGAACCAAACTGTACAAGCAAAACATCCTGCCCTCCTCCACCGCAGATTCCAAATACCCAAGTGATTGA AACCACCGTGAAATACTTGGATGGAGAAAAATTATCTGTTCTTTGCCAAGACAATTACCTAACTCAGGAC TCAGAAGAAATGGTGTGCAAAGATGGAAGGTGGCAGTCATTACCTCGCTGCATTGAAAAAATTCCATGTT CCCAGCCCCCTACAATAGAACATGGATCTATTAATTTACCCAGATCTTCAGAAGAAAGGAGAGATTCCAT TGAGTCCAGCAGTCATGAACATGGAACTACATTCAGCTATGTCTGTGATGATGGTTTCAGGATACCTGAA GAAAATAGGATAACCTGCTACATGGGAAAATGGAGCACTCCACCTCGCTGTGTTGGACTTCCTTGTGGAC CTCCACCTTCAATTCCTCTTGGTACTGTTTCTCTTGAGCTAGAGAGTTACCAACATGGGGAAGAGGTTAC ATACCATTGTTCTACAGGCTTTGGAATTGATGGACCAGCATTTATTATATGCGAAGGAGGAAAGTGGTCT GACCCACCAAAATGCATAAAAACGGATTGTGACGTTTTACCCACAGTTAAAAATGCCATAATAAGAGGAA AGAGCAAAAAATCATATAGGACAGGAGAACAAGTGACATTCAGATGTCAATCTCCTTATCAAATGAATGG CTCAGACACTGTGACATGTGTTAATAGTCGGTGGATTGGACAGCCAGTATGCAAAGATAATTCCTGTGTG GATCCACCACATGTGCCAAATGCTACTATAGTAACAAGGACCAAGAATAAATATCTACATGGTGACAGAG TACGTTATGAATGTAATAAACCTTTGGAACTATTTGGGCAAGTGGAAGTGATGTGTGAAAATGGGATATG GACAGAAAAACCAAAGTGCCGAGACTCAACAGGGAAATGTGGGCCTCCTCCACCTATTGACAATGGAGAC ATCACCTCCTTGTCATTACCAGTATATGAACCATTATCATCAGTTGAATATCAATGCCAGAAGTATTATC TCCTTAAGGGAAAGAAGACAATAACATGTAGAAATGGAAAGTGGTCTGAGCCACCAACATGCTTACATGC ATGTGTAATACCAGAAAACATTATGGAATCACACAATATAATTCTCAAATGGAGACACACTGAAAAGATT TATTCCCATTCAGGGGAGGATATTGAATTTGGATGTAAATATGGATATTATAAAGCAAGAGATTCACCGC CATTTCGTACAAAGTGCATTAATGGCACCATCAATTATCCCACTTGTGTATAAAATCATAATACATTTAT TAGTTGATTTTATTGTTTAGAAAGGCACATGCATGTGACTAATATACTTTCAATTTGCATTGAAGTATTG TTTAACTCATGTCTTCTCATAAATATAAACATTTTTGTTATATGGTGATTAATTTGTAACTTTAAAAACT ATTGCCAAAATGCAAAAGCAGTAATTCAAAACTCCTAATCTAAAATATGATATGTCCAAGGACAAACTAT TTCAATCAAGAAAGTAGATGTAAGTTCTTCAACATCTGTTTCTATTCAGAACTTTCTCAGATTTTCCTGG ATACCTTTTGATGTAAGGTCCTGATTTACAGTGGATAAAGGATATATTGACTGATTCTTCAAATTAATAT GATTTCCCAAAGCATGTAACAACCAAACTATCATATATTATATGACTAATGCATACAATTAATTACTATA TAATACTTTCAAATAAAAGAATCTAAGAAACTTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842070 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842070 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12114 same embryo
	EMAGE:12113 same embryo
	EurExpress:euxassay_008425 same experiment
	MGI:4823827 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source