EMAGE mouse gene expression spatial database

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EMAGE:12137

Fam184b family with sequence similarity 184, member B ( MGI:2442958)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008461. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008461_01	euxassay_008461_02	euxassay_008461_03	euxassay_008461_04

euxassay_008461_05	euxassay_008461_06	euxassay_008461_07	euxassay_008461_08	euxassay_008461_09

euxassay_008461_10	euxassay_008461_11	euxassay_008461_12	euxassay_008461_13	euxassay_008461_14

euxassay_008461_15	euxassay_008461_16	euxassay_008461_17	euxassay_008461_18	euxassay_008461_19

euxassay_008461_20	euxassay_008461_21	euxassay_008461_22	euxassay_008461_23	euxassay_008461_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12137_wholemount_strong.wlz

12137_wholemount_moderate.wlz

12137_wholemount_weak.wlz

12137_wholemount_possible.wlz

12137_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12137_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 04 05

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 09 19 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 09 10 17 18 19 20 21 22

trigeminal v nerve

strong

regional

strong expression: see section 18

spinal cord mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18

cervical ganglion

moderate

regional

moderate expression: see section 09 10 18 19

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 17 18 19

neural retina

strong

regional

strong expression: see section 01 02 03 04 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

esophagus

strong

regional

strong expression: see section 13 14 15

tongue

strong

regional

strong expression: see section 13 14 15 16 17

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

rectum

strong

regional

strong expression: see section 13 14

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9348
Entity Detected:	Fam184b, family with sequence similarity 184, member B ( MGI:2442958)
Sequence:	sense strand is shown >T9348 CGCACGTGCACACGGCCAGCGGTGAGAGCGCGCGAGTCAGAGCATTTCAGTGCAGGGCTCCTTAAAGCGA TGGCATCTGCCCTCAACAGCAAAATTCACCCGCCAGGAACCTGCGCGAGCTCCAAAGCCGACGCCCGCGG TGGCTCGGGCTGGAGAATGGACTGTGATCCGGAGATGCACGTGAAAATGTGCAAGAAGATCGCTCAGCTC ACTAAGGTTATTTATGCCCTCAATACCCGCCAGGATGAGGTAGAGGTCAGCGTGGAGTCAATACGGGAAG CCCACCAGGAGGACCTCCAGGACACAGGGGCTGAAACCAGGACCAGGCTCCCGCAGGAACAGAGCCGCAC CAGTGAAGACGCAGAGACCCTTCTGAAGCGCATTCAGACCCTGGAAAATGCCCTGGAGCTGCAGAAGAGG CTGACACAGGAGGCCCTGGCTGAGTCAGCCTCCTGTAAATTAGAGACCAAAGAGCGAGAGCTGCGGGTCG AGGCTGAGCATGCTGAACGAGTCCTGATACTTTCCAAGGAGATGCTGGAGCTCAAAGCTGATTATGAGAA GAGGCTCCAGCTGCTGACTAGCCACGAGGGTCCCCAGTGGGGCCAGCTCTCCCAGGAGAGCCCGGATGCC ACAGCCGAGTCTAGCCAGAGACCGGAAATGCACCAGGTCCTGCTGGAGGTGGAGCGATTGAGAGCTGAGA ATAAGCAGCTGAGCCAGGACTATGCCCGCAAGGCGGAGGAGCTACAGGCCACCTATGAGAGGGAAAATGA GGCCATCCGTCAGGCCATGCAGCAGTCAGTCAGCGAGGCCTTATGGCAGTGGCAGGAGAAAGAGTCGGGG CTTCGAAAAAACTTCCAGGTCCAGGAGTCGGCCCTGCAAGCTCAGGTCAGGAAGCTGGAAGGGGACCTGG AGCATAGGGGGCGCAAGATAAGTGACCTGAAGAAGTATGCCCAGAAGTTGAAAGAACGGATCCAGGATCT CGATGTGCAGCTAAGGGAGGCTCGGCAGGAGAACTCGGAGTTGAAGAGCACAGCAAGAAAACTTGGCGAG AAGTTGGCTATTGCCAAAGACAGGCTGATGCTGCAGGAGTGTCACGTGACACAGAAAACTGATGACATGA AGACTGAGGATGGAGTCCTGGGGAAAAGAGATGACCTGGAAGCCTGCAGTCTCCATCCTCAGCAGGAACA AGGCTTTCCCAAGTTGTGTCACTGCAGGAATGGAGGCTCAGAAACACAGACCAAGAAAGAGGCAAGTGGT GAAATGGAAAACATGAAGCAGCAATACGAGGAAGACCTTCGTAAAGTCAGACATCAGACTGAAGAGGAGA AGCAGCAGCTCCGAGAGCAGCTGGGGAAGCGGCTGGAAGACTTGGTGAAGAAGCACACCATGGAAATGAA GTCGGTGTGCTCCACAGTGGAGGTGGAGAGAAAGAAGCTGAAAGAAGTGGAAGCTCAATTGGAAGAGGTG AAGACAAAATCAGAAAGAGAAATACAGCAGCTGCAAGAGGAGAAAGCTGCCCTCAGTACGAAGCTCCAGA ATTCTCTACTTGAGGACCCATGCTCAAGGCCAAAGAAACCTGCCCGAGATGAGGGCCTAGAGAAGCTGAC AGATGAAGAAGAAAGCAGCAGCGATGAAGAAGAAAGGACTGGAGAATCAGTCAAGGGAAAAAGTGACCTA CAACCTCCATTTGAGTCTGTGATGAAGGAGAAGGCAGTGGAGATTGGACACCGACCAGAGGACTGGCAAA GCCAAAGGACCAAACTACAAACACAGGCTGCAGAGTGCTTGAACAAGTACTCCACCGACAGCCTCCAGGC GCACCTGCTGGAGCTCCAAGCCCTAGAGGACAATGCCAGACAAGAGCTGCAGGAGGACTGTGAGCAGATG CAAGTCCAGCAGTCTGGGCTTCTTGAGTCCCTGAGGCAGGAGCTGACAGAGCAGCGAGTTGCCTGCTGTG AGCATCAGAAGGCCCTGGAGATGCTACAGAATGAGTTCAGGGCGGTGGGGCCCTTAGGGAAGTGGCAGGC CACCAATCAGTGTCCAGGGGACAGAAGAGACCACACCTTTATTACAGAGGATATGGGTGTTACAGGCCCT CCTGGTTCCCTGCCTTGTGCAGCAGAGAAAGGTCTCCTGGAGGAGAATGCTCAGCTCCAAGACACTGTAC TGCGCCTGCGTGCTGAGGTGGACCAGCACCTGCAGGAGGCGCTGCAGCTGCGCGAGCAGCACCGGCTGCT GGAGGAGGACCAGAAAGCCCAAAGAGCCATGGAGGTGGAGGCACTCCGCCAGGAGCATCGGAAGGAGATG CAGGCCATGGTGGCAGATTTCAGTGGTGCCCAGGCCCGACTCCAGGCCCGGCTGGCTGCTCTGGAAACTG AGCTAAAAGAGTCTGGAGAGAAGGCAGGGAAAGGAACTTCGCGGCCTGAGGATCTGCAGCTCATAGGCCG CCTGCAGACACGGCTGAAGGAGAGAGAAGACATCATCCGGCAGCTAACGGAAGAGAGAAGATTTCATTAC GCAGCCTTCCCCAGTGCAGTGTCCCATCGGAATCGGTCCTTCTCTTTCAATCCCCACCCAGGATACTTGA CCCCTTCCATGAAGAAAAAGAAGATGGAGGAAGTGCCCAGCCGAGTGGTCAGCGTGCCAAACCTTGCCTC CTATGCGAAGAACTTTCTGAGCGGAGACCTGAGCTCCAGGATCAATGCCCCTCCCATAACTAAGTCACCC AGCCTGGACCCGAGTCCCAGCTGTAGCCAGCCTTACAAGCCCACCCAGTTACTAGATGGGAAAACTGCCT CAAGGACCCAAGATGGCGAACCAGCCCAACCCAAAGAAGCCCCACAGAAGCAAGGCTCCCCGCATCAGGA ATGGTTTACCAAGTACTTTTCATTCTAAACTGACCTGGGGGTCTGTTTGAAGGAGGACATTAGAAAATCA TTTCAACCATCTGAAGGAATAAATTAAAACCACCCAACCAGGTAACTAGCTGGGAGTGACTTACATGCGA TGGATAGCTAAAATGAAGTGTGGCACGAACATTTCAGCTTCATTGTGACACCTGCTTATGTAAGAATGCC TATAGTAAGATGAAACAAGCCAGTTATTAGGAAGCACCCATAGGAAAATATTAACCTCTGATGACCAAAT TACCCATTGTATTCCTAGTGCCTGTAATGTCATACTTCACTTGTGAGATATCAGACTTGTGACTGCCATG GTCTGCTTTAAAAATATTTTTTCCTTGGTCATCTCCACTCAAAGCTATGATTATATATATATGCATATGT ACATATGCATATGTATATATAATTCCAAATGCACATCTAACCTCAAAATGAAGAGGAATAAAATAAAAGT TCAATATAAAAGGAAAGCCAATGAGAACAAAACACCAAACCTACCAGGAATGGACTGCAGCACGCTTGGA TTGCATGTTTTGACTTCCAAGGTCTGGCACAGGCCACAGACTGCAGAGGGACACCACCTACCTCAAACAG CACTTCACAAGATTAAGCAGCATACTATCAAGTCTGACTTGGAATGCTCGGTGAACAACTGTGCCTCCTG TTACACATCTCAGCTTCGATGCATTATGCTAAGTCTTTGCAAAGGTTTCTAGACTGTCTGTTCTCTGATG CCAACCGTTGTTGGACCTGTTCTTTGCATCTGTGTTATCAGCCTCAGATGTAAGTAAGGCTTTCTTACTG CATGGACTCCCAGGAGAGCAGGGAACTGCCCATGGTGCTGAAGCTGCACTCTGTCTTACCCTTTGACACA ACTTTTAGTGGGTAGACTTTTCTTCTCTCTGGTGCTGGCCCAGCAAGTGGCCTATCAACAGATGTCAATT CTATGCATTGTCAAATTTTTGCTCTTTGAATAATAAAACTCTGAACTGGACAATAAAAATGTCTTTGCCT CCACACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6850901 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6850901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12136 same embryo
	EMAGE:12139 same embryo
	EMAGE:12135 same embryo
	EMAGE:12140 same embryo
	EMAGE:12138 same embryo
	EurExpress:euxassay_008461 same experiment
	MGI:4824727 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source