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EMAGE:12260

Ccbl1 cysteine conjugate-beta lyase 1 ( MGI:1917516)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260
"Pseudo-wholemount" of euxassay_007009. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007009_01 euxassay_007009_02 euxassay_007009_03 euxassay_007009_04
EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260
euxassay_007009_05 euxassay_007009_06 euxassay_007009_07 euxassay_007009_08 euxassay_007009_09
EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260
euxassay_007009_10 euxassay_007009_11 euxassay_007009_12 euxassay_007009_13 euxassay_007009_14
EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260
euxassay_007009_15 euxassay_007009_16 euxassay_007009_17 euxassay_007009_18 euxassay_007009_19
EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260 EMAGE:12260
euxassay_007009_20 euxassay_007009_21 euxassay_007009_22 euxassay_007009_23 euxassay_007009_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12260Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12260_wholemount_strong.wlz
12260_wholemount_moderate.wlz
12260_wholemount_weak.wlz
12260_wholemount_possible.wlz
12260_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12260_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 13 14 15 16 17
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 18 19 20 21
thyroid gland
moderate moderate
regionalmoderate expression: see section 12 13 16 17
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
strong strong
regionalstrong expression: see section 06 07 08 moderate expression: see section 03 04 05 09 10 21 22 23 24
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 19 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 19
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 19 20 21
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 11
spinal cord
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19
inner ear
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 18 19 20 21
eyelid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
retina
weak weak
regionalweak expression: see section 24
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04
anterior naris
moderate moderate
regionalmoderate expression: see section 13 14 16 17 18
external naris
moderate moderate
regionalmoderate expression: see section 13 18
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 16 17 18 19 20
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 13 16
pharynx
moderate moderate
regionalmoderate expression: see section 15
esophagus epithelium
moderate moderate
regionalmoderate expression: see section 14
pharynx epithelium
moderate moderate
regionalmoderate expression: see section 12 13 14 16
stomach
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 20 21
oral epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 17
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 20 21
liver
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
bladder
strong strong
regionalstrong expression: see section 15 16 17
urethra of male
moderate moderate
regionalmoderate expression: see section 16
larynx
moderate moderate
regionalmoderate expression: see section 13 14 15
trachea epithelium
moderate moderate
regionalmoderate expression: see section 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8414
Entity Detected:Ccbl1, cysteine conjugate-beta lyase 1 ( MGI:1917516)
Sequence:sense strand is shown

>T8414
CAACCTTGGGAATACGTTTGGGGTCGCGGGTTATTGGACAGAGCAGCGCTATTGTTTGCTGAAGCAGAAC
TGTGTGTGGGACTCAGGCCCTGGCTTGGAGCCATTTTCTGGCCTAGACTGTCTGCCCTTCTGTGCCCCTG
AGGGGACGTCTGCAGTGCCTTGGATCTGGGAGGCATGTTCAGGAGTGCAGCAGGCCTCTCGGTGCACCTG
CTGTGACCACTCTGGGGAAGGAAGGCTGGAGCCTCACGCAGCCAGTGCTTGCACCAGTCTCTCACCATGT
CCAAACAGCTGCAGGCTCGAAGGCTGGAAGGGATCGATCACAACCCCTGGGTGGAGTTTACCAGACTGTC
CAAGGAGTATGATGTCGTGAACTTGGGCCAGGGCTTCCCTGACTTCTCACCGCCAGACTTTGCTGTGCAA
GCTTTTCAGCAGGCTACCACTGGGAACTTCATGCTCAACCAGTACACCTCAGCGTTTGGTTACCCTCCAC
TGACAAAGATCCTGGCAAGTTTCTTTGGCAAGCTGCTGGGACAGGAGATGGACCCACTCAAGAATGTGCT
GGTGACAGTGGGAGCCTATGGGGCCTTGTTCACAGCCTTTCAGGCCCTGGTGGATGAAGGAGACGAGGTC
ATCATCATCGAACCTGCTTTTAATTGTTACGAACCCATGACAATGATGGCTGGAGGTCGCCCTGTGTTCG
TGTCCCTGAGGCTGAGTCCTGCTCCTAAGGGGCAGCTGGGATCCAGCAATGATTGGCAGCTGGACCCCAC
AGAACTGGCCAGCAAGTTCACACCTCGCACCAAGATCCTGGTCCTCAACACACCCAACAACCCTCTGGGA
AAGGTATTCTCTAAGAAGGAGCTGGAGCTGGTGGCTGCACTGTGCCAACAGCATGATGTCCTGTGCTTCT
CTGATGAGGTCTACCAGTGGCTGGTCTATGATGGGCACCAGCACATCAGCATTGCCAGCCTCCCTGGCAT
GTGGGAACGGACACTGACCATCGGCAGCGCAGGCAAAAGCTTCAGTGCCACTGGCTGGAAGGTGGGCTGG
GTCATGGGTCCAGATAACATCATGAAGCACCTGCGGACTGTGCACCAGAACTCTATCTTTCACTGCCCCA
CCCAGGCCCAGGCTGCAGTAGCCCAGTGCTTTGAGCGGGAGCAGCAACACTTTGGACAACCCAGCAGTTA
CTTTTTGCAGCTGCCACAGGCCATGGGCCTGAACCGAGACCACATGATCCAGAGCCTGCAGTCAGTGGGC
CTCAAGCCCTTGATCCCCCAGGGCAGCTACTTCCTCATTGCAGACATCTCAGACTTCAAGAGCAGCATGC
CCGACCTGCCTGGTGCTATGGATGAGCCCTATGATACACGCTTTGCCAAGTGGATGATCAAAAACAAGGG
GCTGTCAGCCATCCCTGTCTCCACCTTCTATAGCCAGCCCCATCATAAGGACTTTGACCACTACATCCGA
TTCTGTTTTGTGAAGGACAAGGCTACACTCCAGGCCATGGATAAGAGGCTGTGCAGCTGGAAAGGAGAGC
CCCAAGCCTGAGGCGCTCAGGCCCGCCTCCAACCCCGAGACCTCAGCTGTGCCTTATCACAGAGATGGCG
CTGCAGGGCCCAGATGTGTGACTGGGCATGTTTCCAGGAAAGAGGCCATCTCTTGGGGGTTTCAGCCATC
TTACCCAGTGTCTGGACTGCTATATTGGGTTGGGGGCTGGTTCTGGAACTCAGTCTGCGCCTCTATAGGT
TCTTGTAGAGCCTTGGTTGTTTCTGGCCTCTGCCTGGGACAGTAAAGGATGGAGGATCAGGCCTGGAGCC
CCAGCCCCACCTTAGCCCTGCTGAGGCTCCATGGCTTCTCCACTTCTTCTCATGTGACCTCACAGTGTTG
CTACTGTTCCTAATAAAGTTTTAAGTTATTAGAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5687475 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5687475 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12261 same embryo
 EMAGE:12263 same embryo
 EMAGE:12265 same embryo
 EMAGE:12264 same embryo
 EMAGE:12262 same embryo
 EurExpress:euxassay_007009 same experiment
 MGI:4823661 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS