EMAGE mouse gene expression spatial database

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EMAGE:12261

Lgi3 leucine-rich repeat LGI family, member 3 ( MGI:2182619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007015. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007015_01	euxassay_007015_02	euxassay_007015_03	euxassay_007015_04

euxassay_007015_05	euxassay_007015_06	euxassay_007015_07	euxassay_007015_08	euxassay_007015_09

euxassay_007015_10	euxassay_007015_11	euxassay_007015_12	euxassay_007015_13	euxassay_007015_14

euxassay_007015_15	euxassay_007015_16	euxassay_007015_17	euxassay_007015_18	euxassay_007015_19

euxassay_007015_20	euxassay_007015_21	euxassay_007015_22	euxassay_007015_23	euxassay_007015_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12261_wholemount_strong.wlz

12261_wholemount_moderate.wlz

12261_wholemount_weak.wlz

12261_wholemount_possible.wlz

12261_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12261_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 02 03 04 05 06

foot

moderate

regional

moderate expression: see section 03 04 05 06 24

diencephalon lateral wall mantle layer

moderate

spotted

moderate expression: see section 11 15 16

telencephalon mantle layer

moderate

spotted

moderate expression: see section 02 03 04 05 24

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 14 15 16 17 moderate expression: see section 07 08 09 10 11 12 18 19 20

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13

pons mantle layer

strong

spotted

strong expression: see section 14 15 16 17 moderate expression: see section 05 06 07 08 09 10 11 12 18 19 20

pons ventricular layer

strong

regional

strong expression: see section 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 12 13 14

trigeminal v ganglion

moderate

spotted

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

basal columns

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

stomach mesentery

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

rest of midgut mesentery

moderate

regional

moderate expression: see section 16 17 18 19 20 21

urogenital mesentery

moderate

regional

moderate expression: see section 06 07 08 09 10 24 weak expression: see section 23

left lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

right lung

strong

regional

strong expression: see section 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8407
Entity Detected:	Lgi3, leucine-rich repeat LGI family, member 3 ( MGI:2182619)
Sequence:	sense strand is shown >T8407 TGTTGCGGCAGCCCGGAGGTGGAAGCTCTCGAGAGGGGCAGAACAGCCTGCAGGGAGCAGCGGGGCGCCG GCAGGCTGGTGTGGGGGGAGACCCTGCAGGAGTCATGGCCGGGCTACGAGCCAGGCGGGGCCCTGGGCGC AGGCTACTGGTGTTGTCCACGCTGGGTTTTTGCCTAATGCTGCAAGTCAGTGCCAAGAGACCTCCTAAGA CGCCCCCCTGCCCACCCAGCTGCTCCTGCACCAGGGACACCGCCTTCTGCGTGGACTCTAAGTCGGTGCC CAAGAACCTGCCTTCAGAGGTCATCTCCCTGACGCTGGTGAATGCTGCCTTCTCAGAGATCCAGGATGGA GCCTTCTCCCATCTGCCGTTGTTGCAGTTCTTGTTACTCAACTCTAACAAGTTCACACTGATTGGAGACA ATGCCTTCATAGGACTGTCGCACCTGCAGTACCTCTTCATAGAGAACAATGACATCTGGGCTCTCTCCAA GTTTACCTTCAGAGGACTGAAGTCCTTGACACACCTTTCTCTGGCCAACAATAACCTGCAGACACTGCCC AGAGACATCTTCCGTCCCCTGGACATCCTGAGTGACTTAGACCTCCGGGGCAACGCACTTAACTGTGACT GCAAGGTAAAGTGGCTGGTGGAGTGGCTGGCACACACCAACACCACGGTGGCTCCCATCTACTGCGCCAG CCCGCCCCGCTTCCAGGAACACAAGGTGCAGGACTTGCCTCTTCGAGAATTCGACTGCATCACCACTGAT TTTGTCCTCTACCAGACCCTGTCCTTCCCAGCAGTGTCTGCGGAACCTTTCCTTTACTCTAGTGACCTCT ACTTGGCTCTGGCCCAGCCAGGTGCCAGCGCCTGCACCATCCTCAAGTGGGACTATGTGGAACGACAGCT TCGAGACTATGACAGAATTCCAGCCCCTTCAGCTGTGCACTGCAAGCCGATGGTGGTGGATGGCCAGCTC TATGTCGTTGTGGCCCAGCTGTTTGGTGGCTCATACATTTACCACTGGGACCCCAACACCACACGTTTCA CCAAGTTGCAGGACATCGACCCACAGCGCGTGCGCAAACCCAATGACCTGGAAGCCTTCCGCATCGACGG TGACTGGTTTTTCGCAGTGGCTGACAGCTCCAAGGCAGGTGCCACCAGCCTCTACCGTTGGCACCAGAAT GGCTTCTATTCCCACCAGGCCCTGCACGCCTGGCACCGCGACACCGACCTGGAGTTTGTGGACGGTGAGG GCAAACCACGGTTAATTGTGTCTAGCAGCTCGCAAGCACCTGTCATCTATCAATGGAGTCGTTCTCAGAA ACAGTTTGTGGCTCAGGGAGAGGTGACTCAGGTACCTGATGCCCAGGCCGTGAAACACTTTCGTGCTGGC CGAGACAGCTACCTGTGCCTTAGCCGCTACATTGGCGACTCCAAGATCCTGCGCTGGGAAGGTACCCGTT TCTCTGAGGTGCAAGCCCTTCCCTCCCGAGGCTCGCTGGCCCTACAGCCCTTCCTGGTGGGTGGTCACCG CTACCTGGCCCTGGGCAGTGACTTCTCCTTCACCCAGATCTACCAGTGGGATGAGGGGCGACAGAAATTT GTACGGTTCCAAGAGCTGGCAGTACAGGCACCCCGGGCCTTCTGCTACATGCCTGCTGGAGATGCCCAGC TGCTCCTGGCCCCCAGTTTCAAGGGACAAACACTGGTGTACCGACATGTTGTGGTGGACCTTAGTGCCTA GAGGGGGGCTAAGTCCTTTGGGTTCCTCAGGGTGGCACTGTACGATGGGTGGGGACCTCTGTGAGCAACT TGTGGCCCCAAGTGAAGTCACAGGTGGCCAGCCTGTCTATGTAGCTACACACACATACACAAAGAAGTGG GCCTGGGCACACACCAGGGACTAGCCCAGGGGAGCCATTCCCATTACTGTAATAAGCACCTACGTATGCA CCAGGCACCCAGTGTCCAGGCTCTCTATGTGCGCACCAACAGCCCCTGATCTTCAGTGTTCCCTGCTCTG CTCCGTTGCTAGCGTGTCTGAGTAACAAAGCGAGTGCAGGAAGGGGTGAGCCACAGCCTTCTCTTCTCAG CTTAGCTTTCTGCTCTCTGGCCTCTGGATGCCCGAGGTGCTTGTGTACCTGTTTGGCATCCACAGTTGCA GCCCCCCTGCAGGTGTGCCCACACTTCCAAGTGTGCCTGCCCACGCCATGCTCCTTCTGCATGCACCTGT GGACACTGATGGACAAACTCAGCTGCATACTTGTGCATACCCCACCACACTTCTGATTCTGTATCTGCAC ACGTCTGGGGGTGTGTACGTGAGCGGACTAGGTGCTGGGCAAACCGGCGTCCTTGATACCCCGTAGCTGC TCTTCTTGGAGGGTGCCTACAGCACCCACACTTTCTCTGCTCTCCCCAGTGTGCCCCCTGTGTGGCTCAT AAGGATAGCAACAGAAAACGTGACCATGGCCTCGTGGGGACAAAGCCACTTCATCCAGAAGCAATAATAA CAAGAGGCAGCAGCAACTGGGTTCTTCACAGTGCAGTATAGCTCTCCTCACTAGAGTTTTCTTCCACGAC TCAGTCCCAGCTCAAAGGGACACGGGTGGGAAGGCTTTCTCTGGATAGCTATTGCTTTTTCAAGCTCGGG CCATCTCCTCTCTCACCCTATCTTTGAGATGCGGATCCCAAAGTGTGATTGCTCCTTGACTGGATATATA AAAAAAAATCAGCCAAGGCAAGAATCCTGAGACAGCCTGAGCTACTTGTGTGGGCGCCAGGAACATGTTG GTCGCCCTGGCACCCCTCCATGCCATGGAGATGCCCCCCGGAGGCACAGCTCGGTGGAGGCTGGATGAGG ATGGCAGGGAGGGACACCTGGGAACCAGGCCTCTGCTGCCCTCTGCTGTCCTCTGGGAGCAGCAGCCCTG AAGTAGGAAATGATCAGGCTGAGAGGATTTCTTGCGGCCTGATGCACCTGGGGATAATGCTAAGATTCCT TTGCTTCGAATAAAGTTCCACCCCACTGCTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686778 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686778 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12260 same embryo
	EMAGE:12263 same embryo
	EMAGE:12265 same embryo
	EMAGE:12264 same embryo
	EMAGE:12262 same embryo
	EurExpress:euxassay_007015 same experiment
	MGI:4825913 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source