EMAGE mouse gene expression spatial database

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EMAGE:12366

Slc39a12 solute carrier family 39 (zinc transporter), member 12 ( MGI:2139274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007024. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007024_01	euxassay_007024_02	euxassay_007024_03	euxassay_007024_04

euxassay_007024_05	euxassay_007024_06	euxassay_007024_07	euxassay_007024_08	euxassay_007024_09

euxassay_007024_10	euxassay_007024_11	euxassay_007024_12	euxassay_007024_13	euxassay_007024_14

euxassay_007024_15	euxassay_007024_16	euxassay_007024_17	euxassay_007024_18	euxassay_007024_19

euxassay_007024_20	euxassay_007024_21	euxassay_007024_22	euxassay_007024_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12366_wholemount_strong.wlz

12366_wholemount_moderate.wlz

12366_wholemount_weak.wlz

12366_wholemount_possible.wlz

12366_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12366_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

mesenchyme

strong

spotted

strong expression: see section 01 02 03 23 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14

diencephalon roof plate

strong

regional

strong expression: see section 12 13

cerebral cortex ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 14 15 16 17 18

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 10 11 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

pons ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9440
Entity Detected:	Slc39a12, solute carrier family 39 (zinc transporter), member 12 ( MGI:2139274)
Sequence:	sense strand is shown >T9440 GGAATTTTAAAACGGAGAACTGTGGGACCACAAAATGCTCTCAGAAATTTAAAGGCCCAGGGAGCTAGAG ATAAACAACTATTTCTCTTCTTGAGGAAACCCTCTGGTTGTAAATTTGTCCTGCATGAAGCCTCCCATTC ACCCCCAACACAACCCAAGCCCAAGAGTGGACATGTGCTTCTGGACCAATCTTTCAGTGTGGATGATCCT ACTGTCCCACTCCCTCAGCCTCGTTTCCTCTACTGAGACCGGCAAAACCCTCACTCAGAATAACAGCAGA GCTGGGAGCCAAGGCCTGCTAGAAGTTCTGAGGGTCCTCTCTGCTGGTGACCACTGGTCCCTCAACCATC CACAAAGCCTCATCAAAATTCTGTTGGAGAGGACTGGGTGTCCACAGAGAACAGATTGGACACAAGGAGA CTGCAAGCTGTGTTTGGAAGCAGATGCACTGTTACTCACAGCTGGGGGAAATTTGGAGGATGAGCTAAGA GAGGAGGTAGTCCAGAGAGTTTCTCTTCTTCTCCTCTACTACATAATTCATCAGGAAGAGATCTGTTCTT CCAAGCTCAACATGAGCAACAGAGAATATGAGTTTTACCTGCACAGCCTACTCGGCCTTAGGCAGGATGA AGACTCCTATTTCCTTTCAGAGAAGGAGACTGATGACATCTTGGCTTTCACCAGGAAGTACTTTGGCACT TCCAGTAGCCAGTGTATGGAAACTAAGATTCTGCAGAGGGAATCTGGCATTCAGGGCAGCAATGGTGCTG ATGAAAAGACACTTCCTCAGCTGGCAGCAACCATCATCGCTCTGTCACTCCAAGGAGTTTGTCTGGGAAG AAAAGCCTTGCCTTCTCCAGATGATTTTACAGAGTATATTTTCAGTTTCCTGAATAGTACCAATACCCTC CACCTATCTGAAATAGAGCAACTTCTGAACATGCTCACGACCAGAAGGACCTGTGCTAAAGAGGATAAAT ACCTCCATCAATACCAAAGGAAACAAAACACAGAGGAACACAGTTTGAGAGACCCCAAGACATCTACAGC AATGGACAAAGAGTCTGATGATCATTCTCCTTCCTGGGATCAGGCTTGCTTCTCTGCTAGGCAGTTGGTG GAGATATTTCTACAGAATCATAGCAGTCTGTCCATTTCTAAGGAGGACTTTAAGCAGCTAAGTCCAGGGA TCATCCAGCAACTTCTCAGCTGCTCTTGTCAGATGCCCAAGGACCAGCAAGCAAAGCCCCCACCCACCAC TCTGGAGAAATATGGCTACAGTACAGTGGCAGTGACACTCCTAACCTTGGGCTCCATGCTTGGGACAGCG CTGGTCCTTTTCCACAGCTGTGAGGAGAACTACAGTCTTATTTTACAGCTGTTCGTGGGTTTGGCTGTGG GGACGCTCTCTGGGGATGCCCTGCTCCATCTCATACCTCAGGTTCTTGGTTTACATAAGCAGGAAGCAGA GTTTGGGCATTTCCATGAAAGCCAAAGTCCTATTTGGAAGCTGTTGGGACTACTTGGAGGCATCCATGGA TTTTTCTTGATAGAAAAATGTTTTATCCTTCTTGTATCACCAAATACCAAGGGTCTGCCATTAGTTAATG AACATGTGGGACATACACACCATCTTGGACTCAATCCAGAATTAAATGACCAGTCAAGTGGAGGCAAGTC TATCTCAACCATCCAACTGAAAGGCCCAGAAGATTCACAGACAACTGTGCTACCCATAGGCAATGTGCCA GCCTCCAACAGAAATGGCAAAACCATCAGCTTATTGGCCATTATGATCCTGGTTGGAGATAGCCTGCACA ATTTTGCAGATGGCCTAGTAATAGGCACAGCCTTCTCCTCTTCATTGGAGTCAGGAGTGACCACAACAAT TGCTATCTTGTGTCATGAAATTCCACATGAAATGGGAGACTTTGCAGTGCTCTTAAGCTCAGGACTCTCT GTCAGGACTGCCATCCTGATGAATTTTCTAAGTGCCCTGACGGCCTTCGCAGGACTGTACATTGGTCTCT CAGTATCTGCTGACCCACGTGTCCAAGACTGGATTTTAACAGTGACTGCTGGGATGTTCTTATATTTATC TTTGGTGGGGATGCTTCCTGAAATGACTCATGTTCAGACACAACGACCCTGGATGACGTTTCTCCTGCAG AATGTCGGATTGGTCCTTGGCTGGTTTTCTCTTTTGCTCTTAGCTGTATATGAACAGAATATTAAAATAT AAGTTAAGAATCTCTAATGGCTTCCAAAATGTAATTATCTGCTCATATCTCGTATCTTCCATGTTCTCTA TGCCAATCACTAATTTGGTATTTATATTCGAGGCAAAGAATTTGTCATTTATCTTAACTTATTAATTCTG TATTATAAATTTTTAGAAATATATATATGTGAGTCTGGCTTGGATTAAAATCTTTATTTTTGTTTCTGAC ACTGTGATTGGCCACAATAATGTTTAGTGTTAGAAAATTCTTAATTCTAGTCAGCTGCAAAAATATTATT TCCCTACAAAAGAATGTTTATTCCTTATATTTGAAGCAGACATTTTTGTCGTTGTTTTTTAAATTGTTAA TAATATATTGTTTTCTATGTGATTAGTAAAGCATAAGCAAATATAATTGTAGAAATGTAATAAAGGCTAG TTTCAATTACAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30628112 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30628112 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12368 same embryo
	EMAGE:12370 same embryo
	EMAGE:12371 same embryo
	EMAGE:12367 same embryo
	EMAGE:12369 same embryo
	EurExpress:euxassay_007024 same experiment
	MGI:4828221 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source