EMAGE mouse gene expression spatial database

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EMAGE:12370

Camkv CaM kinase-like vesicle-associated ( MGI:2384296)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007008. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007008_01	euxassay_007008_02	euxassay_007008_03	euxassay_007008_04

euxassay_007008_05	euxassay_007008_06	euxassay_007008_07	euxassay_007008_08	euxassay_007008_09

euxassay_007008_10	euxassay_007008_11	euxassay_007008_12	euxassay_007008_13	euxassay_007008_14

euxassay_007008_15	euxassay_007008_16	euxassay_007008_17	euxassay_007008_18	euxassay_007008_19

euxassay_007008_20	euxassay_007008_21	euxassay_007008_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12370_wholemount_strong.wlz

12370_wholemount_moderate.wlz

12370_wholemount_weak.wlz

12370_wholemount_possible.wlz

12370_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12370_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 2 phalanx

strong

regional

strong expression: see section 02

forelimb digit 3 phalanx

strong

regional

strong expression: see section 02

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 03 04 05 21 22

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 03 04 05 21 22

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 03 04 05 06 21 22

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 03 04 05 06 07 21 22

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 04 05 06 07 21 22

diencephalon

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17

hindbrain

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4352
Entity Detected:	Camkv, CaM kinase-like vesicle-associated ( MGI:2384296)
Sequence:	sense strand is shown >T4352 AGATGCTGGAGTCGAAGCGGCTGCAGCTGGCGCCGCGTCTGCCCCGCGTGCCCGGAGCGGATTCTGCCCG CCGCCCCCGGAGCCCTGCTGAGCGGGTTATCGCTTCCTGCCTGTGACCGACCAGCTTTGCAGAGCCTAGC AATGCCGTTTGGGTGTGTGACTCTGGGTGACAAGAAGAACTATAACCAGCCATCGGAAGTGACTGACAGA TATGACCTGGGACAAGTCATCAAGACTGAGGAGTTCTGTGAAATCTTCCGGGCCAAGGACAAGACAACGG GCAAGCTGCACACCTGTAAGAAGTTCCAGAAGCGTGATGGCCGCAAGGTGCGGAAGGCAGCCAAGAACGA GATTGGAATCCTCAAGATGGTGAAGCACCCCAACATCCTGCAGCTGGTAGATGTGTTTGTGACCCGCAAG GAATACTTCATCTTTCTGGAGCTGGCCACGGGGAGGGAGGTGTTTGACTGGATCCTGGACCAGGGCTACT ACTCGGAGCGAGACACGAGCAACGTGGTGCGGCAGGTCTTGGAGGCTGTGGCCTACTTGCACTCGCTCAA GATTGTGCACAGGAACCTCAAGCTGGAAAACCTAGTGTACTACAACAGGCTGAAGAACTCAAAGATTGTC ATCAGCGACTTTCACCTGGCTAAGCTAGAGAATGGCCTTATCAAGGAGCCCTGTGGAACCCCGGAGTATC TGGCACCAGAAGTGGTAGGACGGCAACGGTACGGTCGCCCTGTAGACTGTTGGGCCATTGGCGTCATCAT GTATATCCTGCTTTCAGGCAACCCGCCCTTCTACGAGGAGGTGGAAGAAGATGACTATGAGAACCACGAT AAGAATCTCTTCCGCAAGATCCTGGCTGGTGACTATGAGTTTGACTCTCCGTACTGGGACGATATTTCTC AAGCAGCCAAAGACCTGGTCACCAGGCTGATGGAGGTGGAGCAAGACCAGCGGATCACCGCAGAAGAGGC CATCTCCCACGAATGGATTTCCGGCAACGCGGCTTCTGATAAGAACATCAAGGATGGCGTCTGTGCCCAG ATTGAGAAGAACTTTGCCAGAGCCAAGTGGAAGAAGGCTGTCCGAGTGACCACTCTCATGAAGCGGCTCC GGGCACCAGAGCAGTCTGGAACAGCGGCGACCCAGTCTGCTTCAGACGCTGCCACTCCTGGGGCTGCTGG TGGGGCCATCGCTGCTGCAGCTGCAGCTGCAGCTGCAGGTGGAGCTGCCTCAGCCTCTGGGGCCAGTGCT ACTGCAGCCACAGAGGGTGGTGCTGGGTGTGCTGCCAAGAGCGATGACATAGCCTCTGCAGATCGTAGTG CTACACCAGCCACAGATGGCAGCGCCACTCCAGCCACAGATGGCAGCGTCACCCCAGCCACGGATGGCAG CATCACCCCAGCCACTGACGGGAGTGTCACTCCAGCTACTGACAGAAGCGCTACACCAGCTACTGATGGC AGAGCCACACCAGCCACAGAAGAGAGCACAGTGCCCGCCACTCAAAGCAGTGCCCTGCCAGCTGCAAAGG CAGCAGCCACCCCTGAGCCGGCTGTGGCCCAGCCGGACAGCACAGCCCTAGAGGGTGCCACAGGCCAGGC TCCACCCTCTAGTAAAGGAGAAGAGGCTACTGGATGTGCCCAGGAGTCTCAGAGGGTGGAGACGAGCTGA ATGGGCAGCCTGTTGGGGGGTTTTGAGGGATGGGAAGGAGGGTGGGAGTTTGGATGAGGGGCTTCTCACT GTATATAGAGTCACTGGCATGATGCCCTAGCTCCCCCCAAGCTCCCTCATCCCAGTGGGGCATGCCTGGG GGCCATGGGAGAGCAGTCTTGTCTCCTGTGTGGATGTATGTGAGTGGCGGGCAGGCCAGAGGCAGGGCCA GCCCCAGCCCCTGCATGGATTCCTTGTGGCTTTCTGTCTTTCGCTAGCTTCACCAGTTTCTGTTCCTTGT GGGATGCTGCTCTAGGGATACTCAGGGGGTCCTTGCTCTCCTTCCCTCCCTGCCTCATCACTCCCCCAGG CAGGCCCTGCAAACCTCATCCTTTCCCAGGCCCTAAATGGGTGGCCTTGCCCTGAGAGCTGGTCCTCCAG CGAGGCCCTGTCAGCAGTGTTAGGCTCCTGCACGGGAAGGTGTGTACCTGTGGTGTTTGGGCTGCTCTAG GAGTGTGCAAGAGAGGATGCAGAAAACCTAGGGCAGTGTGCTTTAAGTCAAGTCTGGTCACGTGGTAGGG AGCAGGCATTGCATGTTAGCTGTGGGGATCCTCAGAAGTGGGAGAGAGAATGGGTAGAAGCTTCCATCTT TTTTCCCTGGGTGGGGGTGGCGATCTTCCCTCTTGAAGATCCTTGTGTTATTTCATAGCCTCCTGAGTCC TGTAGCGGGTTGCTCTGTATCCCGATTCTTAGGAGACTCTAAAGGAAGAGAGAAACATGTCGGACACAGC AGCGACGCCTAGGAGAACAGAGTACAGTGCCCCAGCCTATCTGGCTCCTTCCTGTGAGCTGGATAGGGAG GGCTGGTGTCCCCCCATCCCCCCATCGTCTTCCTAGATGCCATTACCAAGAAAGAGACCTTTGCCATAGC CACCTCATGCAGGCTCAGGCAGTAAATCCTATTCCTCCCAGGGAGGCGTGGCAGCACCTCATAGGCTGCG TTGGGAGCATGCTTTTCACAGCAGCAGATTTTCTGCTCTTAGTGCAGCGATATGGGGAGGCTTTTCCTCT TTGTCTACCATCAGAGGCTGAGGTATTGCTCAGCCATGTGAAGTAGCTCTGGCTGGGATGAGGAAGTAGA CTACAGTTCCCCAGCTCCCTGAGGGATGCTCTCAGCAGGTGCCCTTGGTCAGACCCTACCACAGCCTGAA CAGGCAGCCACGCTGGTCCTTGCCCTTGCTCGGCACTCCATGGTGGTCCTGCCCTTCTCCCTGCATGCCT GTGGGTCTGCTCTGGTATGTGAAGGTTGGTGGGCTAACTGTGTGCCTACTGAACCTGGCAAATAAACGTC ACCCTGCAAAGCCTCTGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527857 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527857 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12368 same embryo
	EMAGE:12371 same embryo
	EMAGE:12367 same embryo
	EMAGE:12366 same embryo
	EMAGE:12369 same embryo
	EurExpress:euxassay_007008 same experiment
	MGI:4823607 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source