EMAGE mouse gene expression spatial database

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EMAGE:12440

Zbtb7c zinc finger and BTB domain containing 7C ( MGI:2443302)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011232. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011232_01	euxassay_011232_02	euxassay_011232_03	euxassay_011232_04

euxassay_011232_05	euxassay_011232_06	euxassay_011232_07	euxassay_011232_08	euxassay_011232_09

euxassay_011232_10	euxassay_011232_11	euxassay_011232_12	euxassay_011232_13	euxassay_011232_14

euxassay_011232_15	euxassay_011232_16	euxassay_011232_17	euxassay_011232_18	euxassay_011232_19

euxassay_011232_20	euxassay_011232_21	euxassay_011232_22	euxassay_011232_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12440_wholemount_strong.wlz

12440_wholemount_moderate.wlz

12440_wholemount_weak.wlz

12440_wholemount_possible.wlz

12440_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12440_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 weak expression: see section 10 11 12 13 14

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 06 07 08 10 14 15 16 17

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 06 16 17

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 18 19 weak expression: see section 07

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17 18

ventral grey horn

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

naris

moderate

regional

moderate expression: see section 12 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 weak expression: see section 08 18 19

esophagus

moderate

regional

moderate expression: see section 12 weak expression: see section 11

mandible

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22

maxilla

weak

regional

weak expression: see section 05 06 07 08 09 17 18 19 20 21

maturing glomerular tuft

strong

regional

strong expression: see section 08 09 10 18 19 20 moderate expression: see section 17

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9260
Entity Detected:	Zbtb7c, zinc finger and BTB domain containing 7C ( MGI:2443302)
Sequence:	sense strand is shown >T9260 GCTGCCCGCTCGACTGATGCTCGGCTGCACAGCCGCTGGAGCAACCCGGGCCACGAGACTCTTGACAGCG GCGCGGCCCGCGCGGCTCGGGGGCGCAGGGACGGACGGACAGCGGCGGCGAAGGAGGAAGCGAGCGCTGC GGCCGGGAGCCAAGTGCGGGAGGCGCGCGGGGTCGGCGGCGCGCAGAGATCTCCTCTGAAGCAGCTGAAT CTCAAATCGCCCCATCCCTGAGGATTCGGCCAGCAAGGAGGGGAAAGAGGATGGCAAGGGGCTCACCCAG GGCTGAGCAGTGAGCCGCAGCTCTTTGAACCTGGAGCTCCGACTGGGAGGCTCTACCACTGGAGTGACTC GGGAAGAATTGATGCTCTGGCCCCCAGCGACATGTACATGGTGTAATGACAGGAAATGAGCAGGTTTGCA GAAAGACTGGTGGCCTAAAACTGAAGCCTAAGGAGTCGGTGCTTGGCTCTGGCTGAGAACATGGCCAATG ACATTGATGAGCTGATCGGCATCCCTTTCCCTAACCACAGCAGCGAGGTGCTGTGCAGCCTCAACGAGCA GCGGCACGCGGGGCTGCTGTGTGACGTGCTGCTGGTGGTCCAGGAGCAAGAGTACCGAACACACCGCTCA GTGCTGGCGGCCTGCAGCAAATACTTCAAGAAGCTCTTCACAGCCGGGAGCCTGGCCAGCCAGCCCTACG TGTATGAGATCGACTTTGTCCAGCCCGAAGCTCTGGCCGCCATCCTAGAGTTTGCCTACACCTCCACGCT CACCATCACCGCCTCCAACGTCAAGCACATCCTCAATGCGGCCAGGATGCTGGAGATCCAGTGCATCGTG AATGTGTGCCTGGAGATCATGGAGCCCGGAGGCAGTGTCGGTGAGGAGGATGACAAGGAGGAGGAGGACG AGGATGACGATGATGAAGATGAAGACGACGAAGAGGAGGAGGAAGAGGAAGAAGAGGAGGAGGAGGATGA CCCAGAGGACTTTGCCGACCAGGAAAACTTGCCTGACCCCCAGGACATCACCTGCCCCCAAAGCCCCTCC AAGACAGACCACCTCACAGAGAAGGACTATTCGGACACACCCAGGGACTTCCCCGACTCCTTCCAGCCCG GAAGCCCTGGCCATTTGGGAGTGATCCGGGACTTCTCCATTGAATCTCTGCTGAGGGAGAACTTGTACCC CAAAGCCAACATCCCTGACAGAAGACCCTCCTTATCTCCGTTTGCCCCGGAATTCTTCCCACACCTCTGG CCAGGTGGCTTTGGTGCCTTTGCTCAGCTGCCTGAGCAGCCCATGGACAGTGGGCCACTGGATTTAGTCA TCAAGAACCGCAAAATCAAGGAGGAGGACAAAGAGGAGCTGGCCCCGCCCCCACCCCCTCCCTTCTCTAG CGACTTCTTCAAGGACATGTTTCCTGACCTGCCCGGTGGGCCGCTGGGCCCCATCAAGGCAGAGAATGAC TATGGTGCCTATCTCAACTTCCTGAGTGCCACCCACCTGGGGAGCCTCTTCCCACCCTGGCCGCTGGTGG AGGAGCGCAAGCTGAAGCCCAAGGCCTCTCAGCAGTGCCCCATCTGCCACAAGGTCATCATGGGGGCCGG GAAGTTGCCCCGGCACATGAGGACCCACACCGGGGAGAAGCCGTACATGTGCAGCATCTGCGAGGTCCGC TTCACCAGGCAGGACAAGCTGAAGATCCACATGCGGAAGCACACGGGGGAGCGGCCCTACTTGTGCATTC ACTGCAACGCCAAGTTCGTGCACAACTACGACCTCAAGAACCACATGCGCATCCACACCGGCGTGCGGCC CTACCAATGCGAGTTCTGCTACAAGAGCTTCACGCGCTCCGACCACCTGCACCGCCACATCAAACGGCAG AGCTGCCGCATGGCACGACCCCGGCGTGGTCGCAAGCCCGCGGCCTGGAGGGCCGCCAGCCTGCTCTTCG GGCCCGGTGGCCCCTCCGCCGACAAGGCAGCCTTCGTGATGCCACCCGCGCTGGGCGACGTGGGTGGCCA CCTGGGCGGCACAGCCGTGTGCCTCCCGGGCCCCAGCCCCGCTAAGCACTTCCTGGCTTCCCCCAAGGGC ACCCTGAGCCTGCAGGAGCTGGAAAGGCAGTTCGAGGAGACGCAGATGAAGCTGTTTGGACGGGCGCAGC TCGAGGCCGAGAGGAATGCGGGTGGCCTCCTGGCCCTGGCGCTGGCCGAGAACGTGGCCGCCACTCGGCC CTACTTCCCATTGCCCGACCCCTGGGCGGCAGGTCTGGCTGGCCTCCCTGGTCTCACCGGCCTCAACCAC GTGGCCTCCATGTCTGAAGCCAACAACTAGGCTGGCCCTGGTCCACCCTAGACCCAAACCCTGTCTTAAG TCCCTCTAAGGCCTGCCCTGCCCCATCCAGTGGGCTCGAACTTTCTAGTTTGCAAACACAAAGGGAAAAT GAAAAAAAAAAATACATTTTAGCTGAGATGCAATTCTCCTGGCCTTCGAGGGACAGCATCTCCTTTTTGT AGGTGTCTGAGCTAGACATCTCTTCCCTGAGGGTTTGGAACCCCCACCTCTCCCTGGCTGCCTCTGGTTG ACATAGAAATACACGTGGTGCCAAGTCTCTGAAGGTTCTGCACTGGTGCCAGGAGCACATTGCAGTGGTA CTGGGCTGTGAGGAGGGAGCATTCTGCCGAGCTGGCCGGGAGGAGCTAGCATGAGGACTGTGTCCTGGCC AGGTACCTTGGTTGAGCCGTTTGACCCTATGGGGGAGTCCCTCCCAACACCGCTTTTTGGAGTCAGGTTC AAGCTCAGAGGTCCCAGATTCAATTGAGTGATGCTTCCGGGCCATTGGGAGAGACCATCCCCACTGGGCA GAAAGGCCTCCAGCAGCCAGGAGCTGGCATGGAGCGAGCCCATTTTATATCCTGCTTGACCTGTGACCTT AGGAGGGTCAACCCTCTGTGCCTCAGAGTCACCCTGCTGTGAAAGGGGGTGGTAACTTACACTCCCCACA CTTCATACCTATTCACATCCATTCCTATGACAAGAGTCCCCTGGAAGGTAGAAGTTTAGCACCCCCAGCG TTACTGGGGAGACTGTCTCTCTTGCTGGAGTGGGTTTCATCGCCTCCATGTGGACATCAGCATGTTAGCT TCTGTGAGACATGTGGCTGAACTAAACAGGAAGGACAGGATCAGAGAAAGATAGGCTAGGCCTTCCTGGC TTTCAAATCCCCACAGGTAGCTCCAGGAATCCCAGATCCTGCCTCACTTCTGACAGGCCACTGCCATATC TAGACATCCCCTGGAAAGGAGAGTCATCATTGGCTCACCCCCACACTCCCTCAGTGTTCAAACCCCTTTT GCTAACACTTGAGGGATGGATGGGGCTAACAGCCACCACCTGCCTGGCTCCCCCCACCCCCACCCCTCCA AATGTCACAGAACCTAGTCATCCAGGCCTCAGCTCTTTGCTCCTTGAGTTGAGGTTTCTCCAGGCCTGGG CGTTGCTGGTGACACCCTTCTACAGGGTTTAAGCCCCTGATCCCCCTTTCCATATTTGACCACAGGTCTC CCGTGGAAGGAGGTAGAGTCCTTTGGGTTGTGATGGGCTGTGTTGCGAGTCACACTCGGGCTGGTTCTGG GTTGGGAACTTTGCTGGCCCACGCTTTCTTGAATTTGTATTTGGTTTCTCGAGATCCCAGGAAGAAGAAT CCACCTGCTTGCAAGGCCTGGGCCTGGAGTCTCAGAGCCCCAAGCTGTGCGGGTCCACGAGACTCAGCTC TTCCTAGATCAACTGCCTTGGCTTCTTCATGTGCAAACGCAAACGCAAAACTGCCAGAGAAGACAGTAGA CGCTATCCCTCCTGAAGCTCCCAAGGGAGCCCCTGCCTGCCAGTGCCACCAGATGACAGTCCACCAGGGA GGATCTTCCTCAGATCTCCGTCCTCATCAGTCACCTGCTGGGCAGCTGCCTCTTAAATTCCTGTCTGCAG TCCAGACAGTCGCCAATATGCACACTTTACCCCAGCAGCCAAGCGGCTGTAGCTGCACCAGACTCAGAAC CAACAGAGGGACCCAGGGGAGGGCATCCGGTGTCAGGCTTCTGTTTTTATTTGTAAAGGTAATGACTTCT TATAAACTCACTTTTTCAGATGACTGGCTAGTTCTTTTTGTCGTTTGTTATTATTTTTTTTTTCTTTCCT GGGCTGCCGTTTGGAATTGTACATTGTAAAATATCCAAAGATGCTGAGTACTGTATGATCAAGCACTTGA AACAAATGGTTGGGGGGAGGGAATGTGGGGGGCGGCTGTGGGGTTTTTTTATTATTATTATTTTGTTTTG TTTATGTTCCTGACTTCCCTGTCTGTCTGTGGGAGACCTTTGGAATTTTTTCTATTTATAAAACTTTTTA TGTGACGCCTCTATGTAAAAATGACACACAATGAGGTTTGCCTTAGTGATTCAAGGGACAATCTTCCGTA ACTTTGGTCGCACGCTGCATCCTGCCCAGAAATGCTCAGATAATTTTCTGGCCTATTTATTAAACAATAT CAACTGACTTGATTAAATGATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837447 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837447 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12443 same embryo
	EMAGE:12441 same embryo
	EMAGE:12439 same embryo
	EMAGE:12442 same embryo
	EurExpress:euxassay_011232 same experiment
	MGI:4829265 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source