EMAGE mouse gene expression spatial database

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EMAGE:12562

Dusp6 dual specificity phosphatase 6 ( MGI:1914853)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011367. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011367_01	euxassay_011367_02	euxassay_011367_03	euxassay_011367_04

euxassay_011367_05	euxassay_011367_06	euxassay_011367_07	euxassay_011367_08	euxassay_011367_09

euxassay_011367_10	euxassay_011367_11	euxassay_011367_12	euxassay_011367_13	euxassay_011367_14

euxassay_011367_15	euxassay_011367_16	euxassay_011367_17	euxassay_011367_18	euxassay_011367_19

euxassay_011367_20	euxassay_011367_21	euxassay_011367_22	euxassay_011367_23	euxassay_011367_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12562_wholemount_strong.wlz

12562_wholemount_moderate.wlz

12562_wholemount_weak.wlz

12562_wholemount_possible.wlz

12562_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12562_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 05 06 07 08 19 20 21 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 17 18 19 20

pituitary gland

moderate

regional

moderate expression: see section 13 14 15

vibrissa

strong

regional

strong expression: see section 05 06 moderate expression: see section 04 07 08 20 21 22 23

cochlea

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20 weak expression: see section 21

cornea

moderate

regional

moderate expression: see section 03 04 23 24

naris

moderate

regional

moderate expression: see section 12 13 15 16

viscerocranium

moderate

regional

Expression in the turbinate bone.

tongue

strong

regional

strong expression: see section 13

hindgut

weak

regional

weak expression: see section 11 12 13 14

midgut

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 15 16

lower jaw molar

strong

regional

strong expression: see section 06 07 08 19 20

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16 17

upper jaw molar

strong

regional

strong expression: see section 06 07 08 19 20

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 10 11 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30172
Entity Detected:	Dusp6, dual specificity phosphatase 6 ( MGI:1914853)
Sequence:	sense strand is shown >T30172 CATCGACCAGGCTGCTTTCTTTCTGTGTGTGGCCCCGGGTGTCAAAAGTGTCACCAGCTGTCTGTGTTAG ACAAGGTTGCCAAGTGCAAAATTGGTTATTACGGAGGGAGAGATTTGCTCCATTCATTGTTTTTTTGGAA GGACAGGACATGCTGTCTCTAGATCCAGCAATAGGTTTGCTTCTGTACCCCAGCCTACCCAAGCAGGGAC TGGACATCCATCCAGATAGAGGGTAGCATAGGAATAGGGACAGGAGCATCTGTTCTTTAAGGCCTTGTAT GGCTGTTTCCTGTTGCATCTGGAACTAACTATATATATTGTCTTCAGTGAAGACTGATTCAACTTTGGGT ATAGTGGAGCCAAAGAGATTTTTAGCTCTGTATTTGCGGTATCGGTTTAGAAGACAAAAAAAATTAAAAC CTGATACTTTTATCTGATTATTGTAAATATTTGATCTTCAATCACTTGACAGTGTTTGTTTGGCTTGTAT TTGTTTTTTATCTTTGGGCTTAAAAGAGATCCAAAGAGAGAAAGAGCAGTATGCCACTTCAAAAGTATAT GGAAAAAAATGTTCTTTTTAATCCAAAGGGTATATTTGCAGCATGCTTGACCTTGATGTACCAATTCTGA CGGCATTTTCGTGGATATTATCACTAAGACTTTGTTATGATGAGGTCTTCAGTCTCTTTCATATATCTTC CTTGTAACTTTTTTTTTCCTCTTAATGTAGTTTTGACTCTGCCTTACCTTTGTAAATATTTGGCTTACAG TGTCTCAAGGGGTATTTTGGAAAAACACCAAAATTGTGGGTTCACTTTTTTTTTTTTTAAATAACTTCAG CTGTGCTAAACAGCATATTACCTCTGTACAAAATTCTTCAGGGAGTGTCACCTCAAATGCAATACTTTGG TTTGGTTTCTTTCCTTTTAAAAAAAAATACGAAACTGGAAGTGTGTGTATGTGTGCGAGTATGAGCGCCG AT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3491528), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12634. Forward Primer - name:012634_F_IRAV04-07_C18_Dusp6, sequence:CATCGACCAGGCTGCTTT; Reverse Primer - name:012634_R_SP6_IRAV04-07_C18_Dusp6, sequence:AATCGGCGCTCATACTCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12567 same embryo
	EMAGE:12564 same embryo
	EMAGE:12563 same embryo
	EMAGE:12565 same embryo
	EMAGE:12566 same embryo
	EurExpress:euxassay_011367 same experiment
	MGI:4824420 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source