EMAGE mouse gene expression spatial database

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EMAGE:12621

Itgb8 integrin beta 8 ( MGI:1338035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011385. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011385_01	euxassay_011385_02	euxassay_011385_03	euxassay_011385_04

euxassay_011385_05	euxassay_011385_06	euxassay_011385_07	euxassay_011385_08	euxassay_011385_09

euxassay_011385_10	euxassay_011385_11	euxassay_011385_12	euxassay_011385_13	euxassay_011385_14

euxassay_011385_15	euxassay_011385_16	euxassay_011385_17	euxassay_011385_18	euxassay_011385_19

euxassay_011385_20	euxassay_011385_21	euxassay_011385_22	euxassay_011385_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12621_wholemount_strong.wlz

12621_wholemount_moderate.wlz

12621_wholemount_weak.wlz

12621_wholemount_possible.wlz

12621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

weak

regional

weak expression: see section 05 22

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 03 05 21 22

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 03 05 20 21 22

hindlimb digit 4 phalanx

weak

regional

weak expression: see section 03 05 20 21 22

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 05 20 21 22

submandibular gland primordium

weak

regional

weak expression: see section 08 09 16 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 03 07 20 21 22 23

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

telencephalon ventricular layer

strong

regional

strong expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 01 02 03 05 17 18 19 20 21 22 23

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13 14 19

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 14 15

brachial plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 moderate expression: see section 02

optic nerve

weak

regional

weak expression: see section 05 06 07 08 09 10 16 17 18

optic chiasma

weak

regional

weak expression: see section 15

nasal cavity

moderate

regional

moderate expression: see section 12 13 14 15

heart valve

weak

regional

weak expression: see section 10 11 12 13 14

testis

weak

regional

weak expression: see section 05 06 07 08 09 19 20 21 22 23

axial skeleton

moderate

regional

moderate expression: see section 17

axial skeleton tail region

moderate

regional

moderate expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37760
Entity Detected:	Itgb8, integrin beta 8 ( MGI:1338035)
Sequence:	sense strand is shown >T37760 TGAGAACCACAGAGGACTCAAAGGACAGTGTGCGGAAGCTGCCCCAGACCCCAAGTGTCCACAGTGTGAT GACAGCAGATGTCATTTTGATGAAGACCAGTTTCCTTCTGAAACTTGCAAGCCACAGGAGGATCAACCTG TCTGCAGTGGCCGAGGTGTTTGCATCTGTGGGAAATGTTTATGTCACAAAACCAAGCTAGGAAGAGTGTA TGGCCAGTACTGTGAGAAGGATGACTTCTCCTGTCCCTATCTCCATGGGGATGTGTGTGCTGGGCATGGG GAGTGTGAAGGTGGCAGATGCCAGTGCTTTAGTGGCTGGGAAGGAGATCGGTGTCAGTGCCCATCAGCCT CAGCACAGCACTGTGTCAACTCCAAGGGCCAAGTTTGCAGCGGGAGAGGCACCTGCGTGTGTGGCAGGTG TGAGTGTACTGATCCCAGAAGCATTGGCCGTCTCTGTGAGCACTGCCCAACCTGCCATCTCTCCTGCAGT GAAAACTGGAATTGTCTGCAGTGCCTTCACCCTCACAATCTGTCTCAGGCTGCCCTTGATCAGTGTAAAT CCTCCTGTGCTGTCATGGAACAGCATCGCATGGACCAAACATCAGAATGTTTATCTGGCCCAAGCTATCT GCGAATATTTTTCATCATATTCATAGTCACATTCTTGATCGGGTTGCTTAAAGTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83991. Forward Primer - name:083991_F_cDNA_Itgb8, sequence:TGAGAACCACAGAGGACTCAAA; Reverse Primer - name:083991_N_SP6_cDNA_Itgb8, sequence:GAACTTTAAGCAACCCGATCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12624 same embryo
	EMAGE:12623 same embryo
	EMAGE:12620 same embryo
	EMAGE:12622 same embryo
	EMAGE:12619 same embryo
	EurExpress:euxassay_011385 same experiment
	MGI:4825658 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source