EMAGE mouse gene expression spatial database

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EMAGE:12629

Adarb2 adenosine deaminase, RNA-specific, B2 ( MGI:2151118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011310. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011310_01	euxassay_011310_02	euxassay_011310_03	euxassay_011310_04

euxassay_011310_05	euxassay_011310_06	euxassay_011310_07	euxassay_011310_08	euxassay_011310_09

euxassay_011310_10	euxassay_011310_11	euxassay_011310_12	euxassay_011310_13	euxassay_011310_14

euxassay_011310_15	euxassay_011310_16	euxassay_011310_17	euxassay_011310_18	euxassay_011310_19

euxassay_011310_20	euxassay_011310_21	euxassay_011310_22	euxassay_011310_23	euxassay_011310_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12629_wholemount_strong.wlz

12629_wholemount_moderate.wlz

12629_wholemount_weak.wlz

12629_wholemount_possible.wlz

12629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 21 22 23 24

hand

strong

regional

strong expression: see section 02 03

foot

strong

regional

strong expression: see section 03 04 05 06 07 22 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 24

axial musculature

strong

regional

strong expression: see section 03 04 05 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

strong

spotted

strong expression: see section 07 19

trigeminal v ganglion

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21

trigeminal v nerve

strong

spotted

strong expression: see section 18

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 15 16 17 18 19

neural retina

strong

regional

strong expression: see section 01 02 03 04 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

heart ventricle

strong

spotted

strong expression: see section 14 15 16 17 moderate expression: see section 08 09 10 11 12 13

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 15 16 17

genital tubercle of male

strong

regional

strong expression: see section 12 13 14

larynx

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9250
Entity Detected:	Adarb2, adenosine deaminase, RNA-specific, B2 ( MGI:2151118)
Sequence:	sense strand is shown >T9250 AGAGCTCCAGGGCGCTCAGGACGCGCGGGAGAATCTTTCCCAGAGCTTTCAGCCTAAGGAAGGGTTCAGC TTTCCAGCGTGCACCCGAGGCTTCCAGCTCATCCGGAACCAAGGGACGGGCAATTATAGAGAAGGTGCTT GCTGAGGAGACCAGTCCTAAAAAGTGAGCCTGAGACTTGATTTGCAAAACCAGAAACACTCCCGACTGCG GCCGCAGCAGCAAGACTAAGAAAGGTGAAATTGCTGAAGGACCAGCTCCTCTCTGGGTCTCCCAACTGGC ATGGCATCTGTCCTGGGGAGTGGCAGAGGGTCTGGAGGGCTAAGCAGTCAACTCAAATGCAAGTCCAAGA GGAGGAGGAGGAGGAGGTCCAAGAGGAAAGACAAAGTCAGCATACTGTCAACCTTCCTTGCTCCTTTCAA GTACCTAAGTCCTGGCACCACAAACACAGAGGATGAAGATAACCTAAGTACCAGCAGTGCTGAAGTGAAG GAAAACCGAAATGTGAGCAACCTGGGGACCAGGCCCCTGCCCCCTGGAGACTGGGCCAGAGGCAGCACAC CCAGCGTTAAAAGGAAAAGGCCGCTGGAGGAAGGAAATGGGGGACATTTCTGCAAACTCCAGCTGATCTG GAAGAAGCTCTCCTGGTCTGTGACACCCAAGAATGCGCTGGTCCAGCTGCATGAGCTGAAGCCTGGTCTG CAGTACCGAATGGTGTCACAGACGGGCCCGGTGCACGCCCCAGTTTTCGCAGTGGCTGTAGAAGTGAACG GCCTCACATTTGAAGGCACGGGGCCCACTAAGAAGAAGGCCAAGATGCGGGCAGCAGAAATGGCCCTGAA GTCCTTTGTGCAGTTCCCCAATGCCTTCCAGGCCCACCTGGCCATGGGCAGTAGTACCAGCCCGTGCACT GACTTCACCTCTGACCAGGCTGACTTCCCAGACACGTTGTTCAAGGAGTTTGAACCATCCTCAAAAAACG AAGACTTCCCAGGCTGTCATCCCGTTGATACTGAGTTTCTGTCCTCTGCCTATCGGCGAGGACGGCTACT CTACCACACCCTGGACCTCATGGGCCAGGCTCTGCCTGACAGGTCCAGGCTGGCTCCGGGAGCCTTGGGT GAGAGGAACCCTGTAGTGGTACTGAATGAGTTACGCTCAGGTCTTCGGTATGTGTGCCTCTCCGAGACAG CCGAGAAGCCCCGAGTCAAGAGCTTTGTCATGGCTGTGTGCGTGGATGGGAGGACGTTCGAAGGCTCTGG ACGCAGCAAGAAGCTGGCCAAGGGCCAAGCAGCACAAGCTGCCTTGCAGGCCCTCTTCGATATCCGGCTG CCTGGTCACATACCCAGCAGGAGTAAAAGCAACCTCTTGCCACAGGATTTTGCAGACTCTGTGTCCCAGC TGGTCACACAGAAGTTCCGTGAACTGACAGTCGGCCTGACATCTGTGTATGCCCGCCACAAAACACTGGC AGGAATCGTCATGACCAAAGGCTTGGACACCAAACAAGCGCAGGTCATCGTTCTGTCCTCTGGGACCAAG TGTATCAGCGGTGAGCACATCAGTGACCAGGGGCTGGTTGTCAACGACTGTCATGCTGAGATCGTGGCGA GGCGGGCATTTCTTCACTTCCTCTACTCTCAGCTGGAGCTGCACTTGAGCAAGCATCAAGAGGACCCCGA GAGATCAATATTCATACGTTTAAAGGAAGGAGGTTACAGGCTGCGAGAAAACATCCTCTTCCATCTCTAC GTGAGCACATCCCCCTGTGGAGATGCCAGAGTCAACTCTCCATATGAAATCACCACAGACTTGAACAGCA GCAAACACATCGTCAGGAAGTTCCGAGGGCACCTGCGCACCAAGATTGAGTCTGGGGAAGGAACAGTCCC TGTGCGGGGCCCCAGTGCAGTCCAGACGTGGGATGGCATCCTATTGGGGGAGCAGCTAATCACCATGTCC TGCACAGACAAGATTGCCAGCTGGAATGTGCTGGGACTACAAGGCGCTCTCCTCTGTCACTTCATCGAGC CTGTGTACCTCCACAGCATCATTGTGGGCAGCCTGCACCACACGGGCCATCTTGCACGGGTCATGAGCCA CAGGATGGAGGGAATCGGCCAGCTGCCTGCCTCATACCGACAAAACAGGCCTCTCCTTAGTGGCGTGAGT CACGCAGAGGCCCGGCAGCCAGGAAAGTCTCCCCACTTCAGTGCCAACTGGGTCGTGGGCAGCGCCGACC TGGAGATCATTAATGCCACCACTGGGAAGAGGAGCTGTGGGGGCTCATCCCGGCTCTGCAAACACGTGTT TTCTGCTTGGTGGGCACGGCTGCATGGTAGGCTAAGCACACGGATCCCCAGCCATGGAGACACACCATCC ATGTACTGTGAGGCCAAGCAGGGGGCTCACACCTACCAGTCTGTTAAACAGCAGCTCTTCAAGGCCTTTC AGAAAGCCGGTCTTGGCACCTGGGTGAGAAAACCACCGGAGCAAGATCAGTTTCTACTAAGTCTCTAAGT CACTAGGCTCCTTCGCTATTGGACCAGAGGGAGATGCCTGGAGAGGATGAAGCCGTATGATGGTGTGGTG TGTCCATCAGTTCTTGACCTTTGTTCTCCTTCACTGTTCAGGAACATGTGGCTCCATGTGTGGATGAGAG ACTAGGTTTGAGTTTGGGTCGGGCTGCTTCTCCCTGGGCTGTTAGTTCCAAGAACCCCATCCACCATAGC TAGAGAGAGAAGCTTTGTTGTGTCAGCCCCATCCCTTACTGGGTATCAAAAGGAAATTCTCTGTTCAGGC AAGGGAGTCCAGAGGCAGGACTGAAAGGTAGTACTAAAGCGTAGCTTCATGCATCTTCCTTTGGACCTCC CCTCCCTTCTCTTCCAAGGGCCTCTCTAGTGGTGTGATCAAGTCTCACCAGCACAAGTTTATATGACATT TCAAGATAAGCCACAGGTGAACAGGAAAGCCAGGGGCTCCCCAACTCCAAAGAACCCAACAGGAATGCTG TATTGTTCTCATTATTCATTCAGACCTTTCCAACTTCTTTCAAGAAGAACTTGAGACAGACACTGTCAAA GAGCAGCCAAAGTGAAAGCTATACTTATGACACAAAGGGATAGTTACCTAAGACAGGAAAGAGTCATAAT TGATGAGCTAAGAAAACTGTTATTAAACTGCCCAATGATAGTATGTTGCAAACAAACCCAAGCCTTTCCA CTTGATGACAACCTCTCCATTGCTTTTATAGATAATGATAGCCACAGAAGTGACAACTGTAGGGTGATTA ACCACTACCCAATAATCTCTCTACTAGGTATGAGTAATGAAACTGAGTTTTAGATCAAATTCTAAATTTT AAGCAATTTTTTATTAGAAAGAAATCACACACACACACACACACACACACACACACACCTCAGTGCAATC TTTCGGTTTGAGGAGCTGCATGCTCTCTGTCATTTTAGGTCCCTTGATCATCATGTCGCATGAATAAAAT AATAATAACCACAAAGATAATGATGACTTGCCATAGCTGACAAAATTAAAGGGACAGAATCATGCCACTT TTAGAACGGACATTAGAGTTCATCGGTAGACATATATTTCTTTAATATTTAGCCTACATCATCATCTCTA AGAGGAAAACAAAATACAGGCAAGAGGAGGCATGTTGCCAACCAATGTTTCTTGAATTAATTGTCTTTAA ACACTTGGAGCTCATTTCTAACATTCATCAAGCTAGTTAACATGCGTCTAATCTTTTCTATTGAACCCCA TGTGACGGGCTGGAGAGATGGCTCAGCAGTTAGGAGCTTTGCTGCTTCTATAGAGGACTCTGGTTGAGTT CCCAGCTTCCACACGGCAGCTTACAGCCATCCATAACTCCAGCCCCAGCAGCTCCACACATCCATGCAGT GCACCTACATAAATGTGGGCAAATTATCATAGACATAAAGTTAAAATAAATAAATCCTTTTTAATCCCAT CTGAGATGCATTACGGATTTCTGTTTCATTTTGTTTTGTTTTTAAAGAATAGGAGGAACCATGTTTAGAA TATGGGAGTCTTGATTAGAATTCTAGTGCATCATCCCCCAATAAAATTCAACCATTACATCAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6835942 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6835942 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12626 same embryo
	EMAGE:12625 same embryo
	EMAGE:12627 same embryo
	EMAGE:12628 same embryo
	EMAGE:12630 same embryo
	EurExpress:euxassay_011310 same experiment
	MGI:4822996 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source