EMAGE mouse gene expression spatial database

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EMAGE:12655

St6galnac6 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 ( MGI:1355316)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011543. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011543_01	euxassay_011543_02	euxassay_011543_03	euxassay_011543_04

euxassay_011543_05	euxassay_011543_06	euxassay_011543_07	euxassay_011543_08	euxassay_011543_09

euxassay_011543_10	euxassay_011543_11	euxassay_011543_12	euxassay_011543_13	euxassay_011543_14

euxassay_011543_15	euxassay_011543_16	euxassay_011543_17	euxassay_011543_18	euxassay_011543_19

euxassay_011543_20	euxassay_011543_21	euxassay_011543_22	euxassay_011543_23	euxassay_011543_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12655_wholemount_strong.wlz

12655_wholemount_moderate.wlz

12655_wholemount_weak.wlz

12655_wholemount_possible.wlz

12655_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12655_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial muscle

weak

regional

weak expression: see section 03 04 20 21

submandibular gland primordium

weak

regional

weak expression: see section 05 06 07 08 14 15 16 17

mandible

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21

maxilla

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30341
Entity Detected:	St6galnac6, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 ( MGI:1355316)
Sequence:	sense strand is shown >T30341 AAACGAAGTGGCACTGGCCCAGCCATTTAACCATGGCCACCTTCCAGCCAGTTCAGGCTGGCTGGAGTAT TTCCCAGCCAATCAAATCAGGGCCTTGATGAGGGTTTTTTTTTCCTCCAGCCAATCGGGGCTGGGGTTAT CTCTTGGCCAATCAGGGATTTGGAATTCTGTATGGTTTAATCGGGTGTCAGGGGGTCTTTCTCGTCTAGT CAGGGTCTGAGCATAGTCTATCAGGGTATAGGGGTCTTTCTGAGTCAATCTGAAGCTTAGGACATGTCTT TTCCCATAAGGCTTTGCTTCAGAGCCCCAGGAATGGGCCCCCAAATCACTCCCTCCTTAGCTGGGACAAT GAGGTCCTGTCCTAAGGATCTGAGGACTTGGTGTTGCCCCACAGTTGCCAGAATCAGAGCTACTGTAGGG GGAAGGGGCTGCCAGGAGGCCAGGCCAAAGGATTTGTGGAGTTCTAGGAGTGGTAGGGAAGCCTAGGAGG TAAGAGGCGGTGTCCAGTTCCCGGTTTCTTCCTGAGTGACCTTAAACAGACCCTCTTGTCTTTCTGGTAT GCTGTGATGACATCATAGCTCCGTATGTGGAGACCCTCACTCTCCGTTATACTTAAGTGTAAATTCCTCA GCTACCATTCCTCGCTGGGGGACTCAGCTCCCTGGGGATGGGGAGCCTGGGGTACCCTTCCCTGCCTCCT TCTAGAAACTTTAGGGTATTTTTGTGCAAGTGCCCCCCATGGTTGGGGGATCCTGACAGAAATGGGGCAA ACATGAAGCTGTTTCTCTAGCCCCTCAATCCAGCTGCCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4985304), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33089. Forward Primer - name:033089_F_IRAV81-84_J19_Siat7f, sequence:AAACGAAGTGGCACTGGC; Reverse Primer - name:033089_R_SP6_IRAV81-84_J19_Siat7f, sequence:AACGGCAGCTGGATTGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12653 same embryo
	EMAGE:12656 same embryo
	EMAGE:12654 same embryo
	EurExpress:euxassay_011543 same experiment
	MGI:4828467 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source