EMAGE mouse gene expression spatial database

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EMAGE:12661

Rgs4 regulator of G-protein signaling 4 ( MGI:108409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011462. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011462_01	euxassay_011462_02	euxassay_011462_03	euxassay_011462_04

euxassay_011462_05	euxassay_011462_06	euxassay_011462_07	euxassay_011462_08	euxassay_011462_09

euxassay_011462_10	euxassay_011462_11	euxassay_011462_12	euxassay_011462_13	euxassay_011462_14

euxassay_011462_15	euxassay_011462_16	euxassay_011462_17	euxassay_011462_18	euxassay_011462_19

euxassay_011462_20	euxassay_011462_21	euxassay_011462_22	euxassay_011462_23	euxassay_011462_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12661_wholemount_strong.wlz

12661_wholemount_moderate.wlz

12661_wholemount_weak.wlz

12661_wholemount_possible.wlz

12661_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12661_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 10 11 12 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 14 15 16 17 weak expression: see section 13

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 22 23 24 weak expression: see section 03 04 05 06 07 08 09 10 18 19 20 21

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 11 12 13 15 16 17 18 19 20 21 22 23 24 weak expression: see section 03 04

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20 21 weak expression: see section 04 05 22 23

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 weak expression: see section 12

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17 18 19 20 21 weak expression: see section 12

facial vii ganglion

strong

single cell

strong expression: see section 05 06 07 22 23

glossopharyngeal ix ganglion

strong

single cell

strong expression: see section 08 09 21 moderate expression: see section 20

trigeminal v ganglion

strong

single cell

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

strong

single cell

strong expression: see section 10

vestibulocochlear viii ganglion

strong

single cell

strong expression: see section 08 moderate expression: see section 20 weak expression: see section 19

trigeminal v nerve

strong

single cell

strong expression: see section 10 11

spinal cord mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 12 13 18 19

cervical ganglion

strong

regional

strong expression: see section 11 19

thoracic ganglion

strong

regional

strong expression: see section 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 18 19 20

neural retina

weak

regional

weak expression: see section 01 02 03 04 24

aorta

moderate

regional

moderate expression: see section 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30178
Entity Detected:	Rgs4, regulator of G-protein signaling 4 ( MGI:108409)
Sequence:	sense strand is shown >T30178 ATGGCCTTCCCTCCTTTGCCTGGGTGCTCAGGGTGTGCTGATTAGCAGCTGGGTTGGTCTAGAGCTGAAT GTTTGAGCTGAATGAAGGAATGTGGTCCAGGCTTTGCTGCCTAGCCCTACCAAGGACTACTGCTTAGGTG AGGGCAGAGGACCTAAAGATTATCTTAATTCTTTCCTCTTTTGATAAGGTGCTCTTCTTTTCCTTCCCCT CAAGAATGTCACCTGTCAGAGGAGCCTCCTTAGTTCCCTGTGGTTGGTTGTTCACTTTAAAAACCACGGC TACCTGCCGTGGTTCTAAGCTACATGCCTACATCTGTAAAGCATCATCGGGGGGAAAGGTTATGTTTTAT CAGGAGGAAGGAAGTTCTCATGAACTGCAAAGCCCTCTTTGGTTTTCTGCTTTGAGGTAAGAGCTTGTTC ACCCCATCCCAAACACCAAGGCTAGCAGAGACCAATGAAAGGAGAGAGGAAAGGAAAAAGAAAGAGAAAA GAAAACAGAAGAGGAGAGGCTATCAGTTGGTAGGACTACTGCTTGGAGCTTTTCAAGGCACGTGAAATAC TTGAAAATTTCTCATTTGTGTTATTTATGATGTTTTGTACAGTGTTTTTATTATTGTATTGTTTTATAAA TGGAGGTACAGGATATTACCCAAATTATTAATGAATGCCCAAGAAGTAATTTCCTTCTCATTCTTCTAAA AGTACTGCCTTTGAGAGCACACACACATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGT GTGAGCACCATTGCGGCTAGTAAGCCAATGTACCAGGCTGCAAAATGAAGACACTGGAATAGTGTGTATC CAAGTCTCGTCGTATTGAAGAAATAATTTTCTAAGGGTACTCGTTTCGTCTCTCTTGACAGTCTTGCTTG TAATGTCCCCAGAGCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3496887), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 8874. Forward Primer - name:ABA_MGC_004_02_F05_F_CGCAAAGTGCAACAGGTG_ABA_REV_00, sequence:ATGGCCTTCCCTCCTTTG; Reverse Primer - name:_R_SP6_ABA_MGC_008_02_B12_ATGGCCTTCCCTCCTTTGCCTGGG, sequence:GGGAGCTCTGGGGACATT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12662 same embryo
	EMAGE:12660 same embryo
	EMAGE:12659 same embryo
	EMAGE:12657 same embryo
	EMAGE:12658 same embryo
	EurExpress:euxassay_011462 same experiment
	MGI:4827721 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source