EMAGE mouse gene expression spatial database

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EMAGE:12922

Col20a1 collagen, type XX, alpha 1 ( MGI:1920618)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013644. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013644_01	euxassay_013644_02	euxassay_013644_03	euxassay_013644_04

euxassay_013644_05	euxassay_013644_06	euxassay_013644_07	euxassay_013644_08	euxassay_013644_09

euxassay_013644_10	euxassay_013644_11	euxassay_013644_12	euxassay_013644_13	euxassay_013644_14

euxassay_013644_15	euxassay_013644_16	euxassay_013644_17	euxassay_013644_18	euxassay_013644_19

euxassay_013644_20	euxassay_013644_21	euxassay_013644_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12922_wholemount_strong.wlz

12922_wholemount_moderate.wlz

12922_wholemount_weak.wlz

12922_wholemount_possible.wlz

12922_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12922_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21

ventral grey horn

weak

regional

weak expression: see section 10 11 14 15

otic capsule

weak

regional

weak expression: see section 07 08 15 16

nasal septum

moderate

regional

moderate expression: see section 10 11 12

mandible

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 16 17 18

meckel's cartilage

moderate

regional

moderate expression: see section 12

axial skeleton

moderate

regional

moderate expression: see section 14 weak expression: see section 10 11 12 13

basioccipital bone

moderate

regional

moderate expression: see section 08 09 10 12

basisphenoid bone

moderate

regional

moderate expression: see section 08 09 10 12

exoccipital bone

moderate

regional

moderate expression: see section 03 04 05 06 20 21

temporal bone

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 weak expression: see section 18

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 04 05 06 16 17 20 21 22 weak expression: see section 18

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

weak

regional

weak expression: see section 07 08 15 16 17 18

pelvic girdle skeleton

weak

regional

weak expression: see section 07 08 09 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31001
Entity Detected:	Col20a1, collagen, type XX, alpha 1 ( MGI:1920618)
Sequence:	sense strand is shown >T31001 GAGAAGGGGGATCAGGGACTTTCAGGCTTGCAGGGCCTCTCTGGCCAGCAAGGCATCCCTGGGAAAACTG GCCTACAGGGACCAAAGGGCATGAGAGGACTGGAGGGACCTGCTGGCCTGCCAGGACCACCTGGCCCCAG GGGGTTCCAGGGCTTGGCAGGGGCCAGGGGCACCAATGGGGAGCGAGGAGCCCCAGGAGCTGTGGGTCCC ACAGGTCTACCAGGGTCCAAAGGTGAACGAGGAGAGAAGGGAGAGCCACAGTCCCTTGCCACCATCTTCC AGCTGGTGAGCCAGGCCTGCGAGTCAGCCATACGGAGTGAGCCTGTGACTATCAGCCACACAAGCAACCC TAGACTGCAGGAGGTGCAGACCCCAGAATCCCTGGAGTGAGCAGGATCTTCCCTCCGCCACTTCAAATTG CCCTGGCCTTCCCCAGCACCATCATCCATAATTTTGTCTCAGCACTAAGAACAACTGTGTGCTGGAGGGG CAGAGAAGGAAACCCAGAGGAAGACCCCGAGGTGGCAGGAGCTGGAAGGGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4481590), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 23286. Forward Primer - name:023286_F_IRAV32-35_H18_1700051I12Rik, sequence:GAGAAGGGGGATCAGGGA; Reverse Primer - name:023286_R_SP6_IRAV32-35_H18_1700051I1, sequence:TCCTCCCTTCCAGCTCCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12921 same embryo
	EMAGE:12924 same embryo
	EMAGE:12919 same embryo
	EMAGE:12920 same embryo
	EMAGE:12923 same embryo
	EurExpress:euxassay_013644 same experiment
	MGI:4823985 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source