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EMAGE:12991

Csrp1 cysteine and glycine-rich protein 1 ( MGI:88549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991
"Pseudo-wholemount" of euxassay_013687. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013687_01 euxassay_013687_02 euxassay_013687_03 euxassay_013687_04
EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991
euxassay_013687_05 euxassay_013687_06 euxassay_013687_07 euxassay_013687_08 euxassay_013687_09
EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991
euxassay_013687_10 euxassay_013687_11 euxassay_013687_12 euxassay_013687_13 euxassay_013687_14
EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991
euxassay_013687_15 euxassay_013687_16 euxassay_013687_17 euxassay_013687_18 euxassay_013687_19
EMAGE:12991 EMAGE:12991 EMAGE:12991 EMAGE:12991
euxassay_013687_20 euxassay_013687_21 euxassay_013687_22 euxassay_013687_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12991Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12991_wholemount_strong.wlz
12991_wholemount_moderate.wlz
12991_wholemount_weak.wlz
12991_wholemount_possible.wlz
12991_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12991_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 16 17 18 19 20
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 19 20 21 22 23
aorta
moderate moderate
regionalmoderate expression: see section 11 12 13 14
esophagus
moderate moderate
regionalmoderate expression: see section 12
tongue muscle
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10
hindgut
strong strong
regionalstrong expression: see section 13 14
midgut
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
lower jaw incisor
weak weak
regionalweak expression: see section 09 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 18 19
palatal shelf
moderate moderate
regionalmoderate expression: see section 13
upper jaw incisor
weak weak
regionalweak expression: see section 09 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 19
bladder
strong strong
regionalstrong expression: see section 11 12 13 14
metanephros
moderate moderate
regionalmoderate expression: see section 07 08 09 10 16 17 18 19 20
ureter
moderate moderate
regionalmoderate expression: see section 11 15
testis
moderate moderate
regionalmoderate expression: see section 05 06 07 08 19 20 21
lung
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 moderate expression: see section 19 20 21 22 23
trachea
moderate moderate
regionalmoderate expression: see section 13
axial skeleton
weak weak
regionalweak expression: see section 13 14 15
clavicle
moderate moderate
regionalmoderate expression: see section 10 11 16 weak expression: see section 09 17 18
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 12 13 14 15 weak expression: see section 11
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31647
Entity Detected:Csrp1, cysteine and glycine-rich protein 1 ( MGI:88549)
Sequence:sense strand is shown

>T31647
ATGAGGAAGCCCCTGGACACAGGCCCACCACCAACCCCAATGCATCCAAGTTTGCCCAGAAGATTGGCGG
CTCTGAGCGCTGTCCCCGCTGTAGCCAGGCGGTCTATGCTGCGGAGAAGGTGATCGGTGCAGGGAAGTCC
TGGCATAAGTCCTGCTTCCGATGTGCCAAGTGTGGCAAAGGCCTTGAGTCGACCACCCTGGCAGACAAGG
ATGGTGAGATCTACTGTAAAGGATGCTATGCCAAAAACTTTGGGCCCAAAGGTTTTGGCTTTGGACAGGG
AGCTGGAGCCTTGGTTCACTCAGAGTGAGGCTGCCACCGCCTGCGCACCCTGCTTACTCCTAAGCTTTTC
ATCAGCGGTCCATTCCCAAGCCTTGGACACCTCCGAGCTCCTCTCCCTCACTCAGCCCCGCTGCACATCA
CTAATGCCTTGGACTTGGGTATCTGGCTTTTTGTGGCTCAGGGATCTGCCTGAGCTCCTTCCCCACGAAG
GGTTTCCTCCTGCCTGGCATTTGATACCATTACCTTTAGGAGACATCTGTCTAGTCGCCCCTTTCCAGAG
ACCAGGTCCCTTTCCCCACACCTCACCCTCCACAGACCTCTGTTTCTAGGCTGAATGTCCACTCTCTACT
GGCCAGGACACCTGAGCCCACATCTTTGGAGACCAATAGAGCAAAGCAGGAGACCTGGGGCAGGGAGGCA
GGACCAGAATTGGCCTAGGGAGGGTTATGGTTTTTGGGTCCCAGAGAGTCTCCTCTGGGTAGGCGGCTGA
GTTCTTGGTGTATCTCAGTCTGGGAAGTCTCCATGAGAGGCAGGGAGGTGCAGATAGGATTGTGGGCCAT
CGGAAGGAGCTCTTTCTCTGGCCCTTGCCTTTGAATGTGGAAATAGTATCTTATCACTTAGGAATGGAGA
ACAAGCCAGTTCAGAGGACCCCAAAGTCTAGAGATTGCCTGACCTCCTTAGGCATCAAGTTCCCAGTTTG
ATGGGCAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2654089), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12379. Forward Primer - name:012379_F_IRAV08-11_H24_Csrp1, sequence:ATGAGGAAGCCCCTGGAC; Reverse Primer - name:012379_R_SP6_IRAV08-11_H24_Csrp1, sequence:CCTTGCCCATCAAACTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12995 same embryo
 EMAGE:12994 same embryo
 EMAGE:12993 same embryo
 EMAGE:12990 same embryo
 EMAGE:12992 same embryo
 EurExpress:euxassay_013687 same experiment
 MGI:4824088 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS