EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13598

Mir145 microRNA 145 ( MGI:2676830)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019231. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019231_01	euxassay_019231_02	euxassay_019231_03	euxassay_019231_04

euxassay_019231_05	euxassay_019231_06	euxassay_019231_07	euxassay_019231_08	euxassay_019231_09

euxassay_019231_10	euxassay_019231_11	euxassay_019231_12	euxassay_019231_13	euxassay_019231_14

euxassay_019231_15	euxassay_019231_16	euxassay_019231_17	euxassay_019231_18	euxassay_019231_19

euxassay_019231_20	euxassay_019231_21	euxassay_019231_22	euxassay_019231_23	euxassay_019231_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13598_wholemount_strong.wlz

13598_wholemount_moderate.wlz

13598_wholemount_weak.wlz

13598_wholemount_possible.wlz

13598_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13598_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 09 10 13 14 15 16 17 18 19 weak expression: see section 08 11 12

telencephalon mantle layer

moderate

single cell

moderate expression: see section 09 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 03 04 05 06 07 10 11 12

olfactory cortex mantle layer

moderate

single cell

moderate expression: see section 13 15 16 17 18 weak expression: see section 10 11

medulla oblongata alar plate mantle layer

moderate

single cell

moderate expression: see section 09 10 16 17 18 19 20 weak expression: see section 08 11

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 09 10 13 14 15 16 17 18 19 weak expression: see section 11 12

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 09 15 16 17 18 19 20 21 22 weak expression: see section 06

pons mantle layer

moderate

single cell

moderate expression: see section 09 13 14 15 16 17 18 19 20 weak expression: see section 07 08 10 11 12

midbrain mantle layer

moderate

single cell

moderate expression: see section 09 13 14 15 16 17 18 19 20 weak expression: see section 07 08 10 11 12

facial vii ganglion

moderate

single cell

moderate expression: see section 22

trigeminal v ganglion

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22 23 24

dorsal grey horn

moderate

single cell

moderate expression: see section 15

ventral grey horn

moderate

single cell

moderate expression: see section 13 14 15 16 17 18 19

dorsal root ganglion

moderate

single cell

moderate expression: see section 13 19 20

esophagus

moderate

regional

moderate expression: see section 15 weak expression: see section 14 16

stomach

strong

regional

strong expression: see section 07 08 09 10 11 12 moderate expression: see section 03 04 05 06 weak expression: see section 02

hindgut

moderate

regional

moderate expression: see section 15 16 17 weak expression: see section 14

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 moderate expression: see section 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70274
Entity Detected:	Mir145, microRNA 145 ( MGI:2676830)
Sequence:	sense strand is shown >T70274 GTCCAGTTTTCCCAGGAATCCCT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-145 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13594 same embryo
	EMAGE:13597 same embryo
	EMAGE:13596 same embryo
	EMAGE:13599 same embryo
	EMAGE:13595 same embryo
	EurExpress:euxassay_019231 same experiment
	MGI:4826219 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source