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EMAGE:13718

Brca1 breast cancer 1 ( MGI:104537)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718
"Pseudo-wholemount" of euxassay_019531. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019531_01 euxassay_019531_02 euxassay_019531_03 euxassay_019531_04
EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718
euxassay_019531_05 euxassay_019531_06 euxassay_019531_07 euxassay_019531_08 euxassay_019531_09
EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718
euxassay_019531_10 euxassay_019531_11 euxassay_019531_12 euxassay_019531_13 euxassay_019531_14
EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718
euxassay_019531_15 euxassay_019531_16 euxassay_019531_17 euxassay_019531_18 euxassay_019531_19
EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718 EMAGE:13718
euxassay_019531_20 euxassay_019531_21 euxassay_019531_22 euxassay_019531_23 euxassay_019531_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13718Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13718_wholemount_strong.wlz
13718_wholemount_moderate.wlz
13718_wholemount_weak.wlz
13718_wholemount_possible.wlz
13718_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13718_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 13 14 15 17 18
submandibular gland primordium
weak weak
regionalweak expression: see section 09 10 11 12 13 21 22 23
pancreas
weak weak
regionalweak expression: see section 10 11 12 13 18 19
thyroid gland
weak weak
regionalweak expression: see section 13 14 18 19
vibrissa
weak weak
regionalweak expression: see section 09 10 11 23
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 13 14 15 16
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 13 14 15
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 13 14 18 19 weak expression: see section 12
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 02 03 05 06 09 10 11 13 14 16 17 18 19 weak expression: see section 07 12 21 22 23 24
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07 09 11 13 17 18 19 21 22 23
rest of cerebellum ventricular layer
weak weak
regionalweak expression: see section 10
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 13 15 weak expression: see section 10 11 12 14 16 17 18
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 13 14 15 17 18
vomeronasal organ
weak weak
regionalweak expression: see section 15
lower jaw incisor
weak weak
regionalweak expression: see section 14 15 19
lower jaw molar
weak weak
regionalweak expression: see section 09 10 21
upper jaw incisor
weak weak
regionalweak expression: see section 14 15 16 19
upper jaw molar
weak weak
regionalweak expression: see section 09 10 21
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 09 10 11 13 14 15 17 18 19 21 22 23 weak expression: see section 04 07 12 16
metanephros
weak weak
regionalweak expression: see section 09 10 11 18 19 21 22 23
testis
weak weak
regionalweak expression: see section 07 09 21 22 23 24
lung
weak weak
regionalweak expression: see section 05 06 07 09 10 11 13 14 15 17 18 19 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55014
Entity Detected:Brca1, breast cancer 1 ( MGI:104537)
Sequence:sense strand is shown

>T55014
AGATGACGAGTCAGGGATGGGAAGGCCTTCCCCTTTTAAATCTCCGTTGGCAGGCAGTAGGGGCTCTGCA
CATGGCTGCTCTAGGCATCTTCAAAAGAGAAACTCCCCCTCTCAGGAGGAGCTCCTCCAGCCTGCTGGAT
CAGAGGCGTCATCTGAGCCACACAATTCAACAGGGCAGTCTTGCCTGCCAAGGCGAGAGCTAGAAGGAAC
CCCATACCTGGGATCTGGAATCAGCCTTTTCTCTAGTAGAGACCCCGAATCTGAGTCCCCTAAAGAGCCA
GCCCACATTGGCACCACACCAGCTTCAACCTCTGCACTGAAAATACCCCAAGGTCAAGTTGCTTTCCGGA
GTGCAGCTGCTGCTGGTGCTGATAAAGCAGTGGTAGGAATTGTGAGCAAGATAAAGCCGGAATTGACATC
TTCAGAAGAAAGAGCGGATAGAGACATATCCATGGTGGTGTCAGGCTTGACCCCCAAAGAAGTAATGACC
GTGCAAAAGTTTGCTGAAAAATACCGCCTCACTTTAACTGACGCAATTACTGAGGAGACTACACATGTAA
TTATAAAAACAGATGCGGAGTTTGTGTGTGAGCGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAA
GTGGATAGTTAGCTATTCATGGGTGGTCCGGTCTATCCAAGAAAGAAGACTTCTGAATGTGCATGAATTT
GAAGTCAAAGGAGATGTTGTGACTGGAAGAAATCACCAAGGTCCAAGGCGATCCAGAGAATCCCGGGAAA
AGCTCTTCAAGGGCCTACAGGTCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAG
GATGCTGCAGCTGTGTGGGGCTTCCGTGGTGAAGGAGCTTCCATCGCTCACCCATGACACAGGTGCTCAT
CTAGTTGTGATCGTGCAGCCAAGCGCCTGGACAGAAGACAGCAACTGCCCAGATATTGGGCAGCTGTGCA
AGGCACGTCTTGTGATGTGGGACTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGATGACGAGTCAGGGATGG; Reverse Primer - name:unspecified, sequence:CAGTCCCACATCACAAGACG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13720 same embryo
 EMAGE:13719 same embryo
 EMAGE:13716 same embryo
 EMAGE:13717 same embryo
 EurExpress:euxassay_019531 same experiment
 MGI:4823498 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS