EMAGE mouse gene expression spatial database

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EMAGE:13851

Rfx2 regulatory factor X, 2 (influences HLA class II expression) ( MGI:106583)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019670. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019670_01	euxassay_019670_02	euxassay_019670_03	euxassay_019670_04

euxassay_019670_05	euxassay_019670_06	euxassay_019670_07	euxassay_019670_08	euxassay_019670_09

euxassay_019670_10	euxassay_019670_11	euxassay_019670_12	euxassay_019670_13	euxassay_019670_14

euxassay_019670_15	euxassay_019670_16	euxassay_019670_17	euxassay_019670_18	euxassay_019670_19

euxassay_019670_20	euxassay_019670_21	euxassay_019670_22	euxassay_019670_23	euxassay_019670_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13851_wholemount_strong.wlz

13851_wholemount_moderate.wlz

13851_wholemount_weak.wlz

13851_wholemount_possible.wlz

13851_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13851_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

moderate

regional

moderate expression: see section 14 weak expression: see section 13

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 13 18 19

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 10 11 12 13 16 17 18 19 20 21 22 23 24

metencephalon floor plate

weak

regional

weak expression: see section 13 14

rest of cerebellum ventricular layer

weak

homogeneous

weak expression: see section 08 09 11 12 16 17 19 20

pons ventricular layer

weak

homogeneous

weak expression: see section 08 09 10 11 12 16 17 18 19 20

midbrain floor plate

moderate

regional

moderate expression: see section 14 weak expression: see section 13

spinal cord floor plate

moderate

regional

moderate expression: see section 14 15 16 weak expression: see section 13

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 20

liver lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55166
Entity Detected:	Rfx2, regulatory factor X, 2 (influences HLA class II expression) ( MGI:106583)
Sequence:	sense strand is shown >T55166 CACAGCAGTGAGGCAGATGTGGATGGCCGCAGCCTGGGTGAGCCCCTCGTGAAACGGGAACGGAGTGACC CCAGCCACCCACTGCAGGGCATCTAGCCCCTTCCCTGGGCGTCTTCCACGTGCTTCCGGAAGGCGTCACC TGGCCCTCTAGGGACCTGGACTCTCAGCTGTATCACACTAGTGCCTCTTCAATGATGCCATGTTTACTGT GACCCAAGGTGGCATGGGGTCCCCACCCACAGGGTGCCAGAGACTGTGGGCAACTAAGATGGTCACTCAA GCTGCCATACTCTGCTAAAGTGGCCCTGACCCTGGGCTGTCCCCAAAGGATACAGCAGCCTGCTGTGTGT ATGTGAGTTGTGGGCACATCCCCAGAAATGTCCCAACACCTCGGTGGCTTTCCCACTCAGCTAACCACCA TGTCAAAGAAAAACCACTCCAACAGAGCCCAGAGGGGTAGGAGCCTACTATTCTCTGGCCATTGTGTGGA GGCTCTGCAGGTCCCCTGCGCTGTAGTCTCCATGCATCTAATGGCCTCTCATGGACAGCCAAGAACTCCA GGCACACACCCTGGATACATGCTAACGGCATCTCATCTCATCCTGGACAGGTCCAAGCCCCAAAGAGATA ACAGGCTCCAAGCAGGGCCAGTCAAGCCCAGATGATCGACCCATCTGCTTGACTATAGACTTTCTGTCCA ATTCATGGTTAATACCAAGATAACCTGGGGGGCCTGTGTTAGGGTTCCCCAAGTAGCCACAGGGGTCAGC ACCATATCCACGCCTCCCAACAAAACTCCAAAACCTGTGCTCAGGGCAGTGCCTGAGCCCCTGCAGTCTT CCAGACACCTCCCCCAAACTAGCCATGCTTGCTGCTGCCAGTGGCCAACCCCTTCTCAACCCTAGACCAT GAGATCTCTGCAGACAAATGAACACCAATGCCTATTGGGCCCTGAGTTCTAGAGGCCCTAACATGTTACA CCTTGCTGCTGTCGTC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CACAGCAGTGAGGCAGATGT; Reverse Primer - name:unspecified, sequence:GACGACAGCAGCAAGGTGTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13850 same embryo
	EMAGE:13849 same embryo
	EMAGE:13848 same embryo
	EurExpress:euxassay_019670 same experiment
	MGI:4827705 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source