EMAGE mouse gene expression spatial database

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EMAGE:14042

Ppfibp2 PTPRF interacting protein, binding protein 2 (liprin beta 2) ( MGI:894649)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007827. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007827_01	euxassay_007827_02	euxassay_007827_03	euxassay_007827_04

euxassay_007827_05	euxassay_007827_06	euxassay_007827_07	euxassay_007827_08	euxassay_007827_09

euxassay_007827_10	euxassay_007827_11	euxassay_007827_12	euxassay_007827_13	euxassay_007827_14

euxassay_007827_15	euxassay_007827_16	euxassay_007827_17	euxassay_007827_18	euxassay_007827_19

euxassay_007827_20	euxassay_007827_21	euxassay_007827_22	euxassay_007827_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14042_wholemount_strong.wlz

14042_wholemount_moderate.wlz

14042_wholemount_weak.wlz

14042_wholemount_possible.wlz

14042_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14042_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 21 22 weak expression: see section 04 05 06 07 17 18 19 20

radius

weak

regional

weak expression: see section 01 02

humerus

weak

regional

weak expression: see section 01 02 03 22 23

fibula

weak

regional

weak expression: see section 03 04 05

tibia

weak

regional

weak expression: see section 02 03 04 05

femur

weak

regional

weak expression: see section 02 03 04 05 07 08 19 20 21

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 13 14 16 17 18 19 20

4th ventricle

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 19

tongue epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

mandible

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06 07 08 09 10 15 16 17 18 19 20 21 22

maxilla

weak

regional

weak expression: see section 06 07 08 09 10 15 16 17 18 19 20 21

palatal shelf

weak

regional

weak expression: see section 10 16 17

bladder

moderate

regional

Expression in the fundus region.

orbito-sphenoid

moderate

regional

moderate expression: see section 23 weak expression: see section 02 03 04 05 06 20 21 22

clavicle

moderate

regional

moderate expression: see section 08 15 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3137
Entity Detected:	Ppfibp2, PTPRF interacting protein, binding protein 2 (liprin beta 2) ( MGI:894649)
Sequence:	sense strand is shown >T3137 TGGCCTCGAGNCAGATTCGGACGAGGCTGAACAGGAAAAGCGAGACAAAATGGCCTCACCCGCCTACACA CCTCTGACCACCACCGCCAAAGTTCGGCCTAGGAAACTTGGATTTTCACATTTTGGAAACATGAGAAAAA AGAAGTTTGATGAATCTACAGATTACATTTGCCCCATGGAGCCTGGAGATGCTGTCAGTGACAGCCACAG GGTCTACGGAGTCTACCGGGGCCTCAGTCCCCTTGACAACCATGAACTAGATGGTTTGGACCAGGTGGGA CAGATAAGCTGATGACCCTGTTATCTGCCTTCTCTGTGCACGGAGAGCTCACAGTAACACTGTGTGTCAC CACGTAACTGCACTTCACCCTCGTCCTTTTGCATGATCTACAGAAAACAATTGTGTCTTTGGGCTGGTCT CACTACACACTTTAAGGATGGTCAGAATATGCAGTTATACAGCCACAAAGGGGACAGACTTTGGGGAACT ATAGCCAAATGTGGACTCTGGGAAAACACCTGCAGACATTAGTAGATGTTTAGTAATTCATA
Notes:	The probe template was PCR amplified from IMAGE:1547715 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1547715 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14041 same embryo
	EMAGE:14040 same embryo
	EMAGE:14037 same embryo
	EMAGE:14038 same embryo
	EMAGE:14039 same embryo
	EurExpress:euxassay_007827 same experiment
	MGI:4827355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source