EMAGE mouse gene expression spatial database

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EMAGE:14070

Ak2 adenylate kinase 2 ( MGI:87978)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001711. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001711_01	euxassay_001711_02	euxassay_001711_03	euxassay_001711_04

euxassay_001711_05	euxassay_001711_06	euxassay_001711_07	euxassay_001711_08	euxassay_001711_09

euxassay_001711_10	euxassay_001711_11	euxassay_001711_12	euxassay_001711_13	euxassay_001711_14

euxassay_001711_15	euxassay_001711_16	euxassay_001711_17	euxassay_001711_18	euxassay_001711_19

euxassay_001711_20	euxassay_001711_21	euxassay_001711_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14070_wholemount_strong.wlz

14070_wholemount_moderate.wlz

14070_wholemount_weak.wlz

14070_wholemount_possible.wlz

14070_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14070_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 01 02 03 17 18 19

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 14 15 16

foregut-midgut junction

weak

regional

weak expression: see section 09 12

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 11 12 16 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

hindgut

weak

regional

weak expression: see section 09 10 11 12

rectum

moderate

regional

moderate expression: see section 10

midgut

weak

regional

weak expression: see section 09 10 11 12

lower jaw molar

weak

regional

weak expression: see section 07 16

upper jaw molar

weak

regional

weak expression: see section 07 16

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

renal cortex

weak

regional

weak expression: see section 04 05 06 07 13 14 15 16

nucleus pulposus

moderate

regional

moderate expression: see section 10 11

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 05 06 07 20 21 weak expression: see section 03 04 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2758
Entity Detected:	Ak2, adenylate kinase 2 ( MGI:87978)
Sequence:	sense strand is shown >T2758 TGCAGATAAGGAGACATGGTATTATTTTTAAGCAGTCAGCCTCTGCTTTTCTCTGGACCACAAAGCTAAT GCTCTCTAAGAGACCTCAGCTTCTACATAAGAACCTCATGGATCCCCAGGCCCAGAGCACAGCAGTCAGG CTGGGCCTGTGCATCCCGTGCAGAGGAGGGGGCTGCTGACTGCCGGGGGCTCCCCACTCCATAAAGCGAG AAGACTGACCTGCGCGGATGGAAGAGACGGGGTGTGAGGTTATGGGTGCCGCTCAGCAGACCATCTCTCC TGGAATTGTGGGGAGCAAGGGGAAACAGATCCCACACTTAAGACTTCAGAGTTGCTTGCTCTAGTGACAA AGCCTTCAGCCACTCAGCACTCCCTAGTCTTAAAACCTTGCCTTCATTCATCCACGGGACCCTTTTAGAT AACTGAACAAGGCCTAGGAGTGTAGCTCAGTGGCGTGTGTGTGCCTGGCTCTGTAAAGCCCCGGCTTCAG TCCTCTACAGGGAAGGGTTGGGGGAAAAGCTAAAATGTCTTAAGCTAAGTCAT
Notes:	The probe template was PCR amplified from IMAGE:1514531 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514531 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14069 same embryo
	EurExpress:euxassay_001711 same experiment
	MGI:4823057 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source