EMAGE mouse gene expression spatial database

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EMAGE:14092

Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1 ( MGI:1926119)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001737. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001737_01	euxassay_001737_02	euxassay_001737_03	euxassay_001737_04

euxassay_001737_05	euxassay_001737_06	euxassay_001737_07	euxassay_001737_08	euxassay_001737_09

euxassay_001737_10	euxassay_001737_11	euxassay_001737_12	euxassay_001737_13	euxassay_001737_14

euxassay_001737_15	euxassay_001737_16	euxassay_001737_17	euxassay_001737_18	euxassay_001737_19

euxassay_001737_20	euxassay_001737_21	euxassay_001737_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14092_wholemount_strong.wlz

14092_wholemount_moderate.wlz

14092_wholemount_weak.wlz

14092_wholemount_possible.wlz

14092_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14092_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 03 04

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 03 04 05

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 03 04 05

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 04

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 04

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14

vibrissa

moderate

regional

moderate expression: see section 05 weak expression: see section 04 06 19

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 16 weak expression: see section 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 weak expression: see section 12 15

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09

hindgut

moderate

regional

moderate expression: see section 12 13

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 14

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 15

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 16 18

urethra of male

moderate

regional

moderate expression: see section 12

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 07 08 12 13 14 17 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4104
Entity Detected:	Pdk1, pyruvate dehydrogenase kinase, isoenzyme 1 ( MGI:1926119)
Sequence:	sense strand is shown >T4104 CGGACGCGTGGGCGGGCAGCGGCATACAGCCGCAGGTTGGCGCCCGGAGCTCCGGCTCCTCCGGCGGGGC TCGGTATGAGGCTGGCAAGGCTGCTGCGGGGCGGCACGAGCGTCAGGCCGCTCTGCGCCGTCCCCTGCGC CAGCCGTAGCCTGGCCTCGGCCTCGGGTTCCGGGCCGGCGTCGGAGCTTGGCGTTCCGGGCCAGGTGGAC TTCTATGCGCGCTTCTCGCCGTCGCCACTCTCCATGAAGCAGTTCCTGGACTTCGGGTCAGTGAATGCTT GTGAAAAGACCTCGTTTATGTTTCTGCGACAAGAGTTGCCTGTTAGATTGGCAAATATAATGAAAGAAAT CAGCCTTCTCCCCGATAATCTTCTCAGGACCCCATCCGTACAGCTGGTGCAAAGTTGGTATATCCAAAGC CTTCAGGAGTTGCTTGATTTTAAAGACAAAAGTGCTGAAGATGCTAAAACTATTTATGAATTCACAGACA CAGTGATAAGGATCAGAAACCGGCACAATGATGTCATTCCCACCATGGCCCAGGGTGTGACTGAATACAA GGAGAGCTTCGGGGTGGATCCTGTCACCAGCCAAAATGTTCAGTACTTTTTGGATCGATTCTACATGAGT CGCATCTCAATTAGAATGCTACTCAACCAGCACTCCTTATTGTTCGGTGGAAAAGGAAGTCCATCTCATC GAAAGCACATTGGAAGCATAAATCCAAACTGCGACGTGGTGGAGGTCATTAAAGATGGCTATGAGAACGC TAGGCGGCTTTGTGATTTGTATTATGTTAACTCTCCTGAACTTGAACTTGAAGAACTAAATGCGAAATCA CCAGGACAGACAATACAAGTGGTTTATGTACCATCCCATCTCTATCACATGGTGTTTGAACTGTTCAAGA ATGCCATGAGAGCGACCATGGAGCACCACGCGGACAAAGGCGTTTATCCCCCGATTCAGGTTCACGTCAC GCTGGGCGAGGAGGATCTGACTGTGAAGATGAGTGACCGGGGAGGCGGTGTTCCACTGAGGAAGATCGAC AGACTCTTCAACTACATGTACTCAACCGCACCCCGGCCTCGTGTTGAAACGTCCCGTGCTGTGCCCCTGG CTGGGTTTGGTTACGGATTGCCCATATCACGCCTCTATGCACAGTACTTCCAGGGAGACCTAAAGCTGTA TTCCTTAGAGGGCTACGGGACAGATGCGGTTATCTACATTAAGGCTCTGTCGACAGAATCCGTCGAGAGA CTCCCTGTGTACAATAAAGCCGCCTGGAAGCATTACAAAGCCAACCACGAGGCTGACGACTGGTGTGTGC CCAGCCGAGAGCCGAAAGATATGACCACATTCCGAAGCTCTTAAACGCACACGGGACGCCTGGCACATCC ACATGTGGTTTCTGCTAAATTTGGAAGACATGTATTTGGAACCACACTGTTCCAAATATTTCACGTGTGA TCTTCACAATTAGTGTTTGCTGAAGCTCCTAAAGGATCGGTTATGCCAACTGTATTCTGATGTGTTGGGT TAATTGGGCGTGGTTTTGAACACACATAATTTTGGTCAACTACCCTTGGTATGGTATGGGGTGTGAAGTT CCTGGTTTCTGTAGGAAGCTGAGTATTTCTTTCAAGTTTTCACTTACTTCTGCTAGAAGCATCTGAAGAA CGTGGAGTAGCTGGCTAGCGGCCTCTCTGCTTTTGGAGTTCTATCATCTGTGAGTGGTGACACCTAGTCA GATGGTCTTCTTATCTCCCGCGAAGAACAACACAGTGATCCGTTAGAACCCACACAGGTTGTCAGAGTGC TGAAATGAATTGATCATGGCATCATTTTTTTGCAGTGGATTGTAATGACTGGTTCCCCATGGAGCTAAGT CCAGAGCATGGTTATCGAGTTTACAGTCAGCGCAAGGGATTTTCTTTAAAAACAAAGTCTCACTGTGTAG CCCCAGCTGGCCTTGAGCTTACAAAGCCCTCGGTGTCGGTGTCGTGAGCGCTGGAATGAGAAGGGTGTGC CACCACATCTGGCTTACAAGAGAAACGTTAGTCCCAAAACAAGCCAAATGACAGCAGAGAATAAACTGTT TGGAAAGCACCAAGTGGCCCAGGCCGAGCTGACCGACTGCTGAGGTGATCTGAGGATGAGCGTGAGATTC TCTGGGTCCCACCCAGGGATCCAGAGGCTGAAGGCCAGGGACCAGGTGAGGCTCATGGCTGGCCTGGCTG GAAGTATATTTGGGAGTGAATCGTGACTACTGCTGTTGGCAGAGTGACAGACTAGATTGGGAAGCTTGTC GGGTTGCTACACATTAGGGGACACAATCTGAGTCTCAAGCCTGGTGCAAGCTAAAGCATTAGCAGGAAGA CCAGGGTGACAGCGAGAGGTCAGGTGACCAAAGGCTCAGCCACCTTGGTTTTCAGTGGTGACTAAGAACA TGTATTTTTCTAAAATATCCAGATTTTTTTTTTTACATCAAAGAAAAGTCAGCTTATATTGATTACAGAA TTCATTTGCAGCTATGTGGTGATTTAAAGTCTGCAACAAACGCTTTGAAGTACTGATGTTATTTGTGTTA AAATACAAACCTATGGAGCAGGTAATGACACCTGGGTTCTCTCTCAGTGACCCAGAGTCACACAGAATTG GCGCCCCAGCCTGAGCTGTCTCTGCTGATCACTGTCCACTGTCTGGGATATTTATAACCTCCTCTGTTAG GTGATGGGGAAGCACATGGAAAACATGCTTTGCCACTTGCCTAGAGGGATTTGGGGGTTTGGTTTGGAAA ATATAGTGCGTTAAAACATGTCACTCTTGACAGGATTTATCCAGTCTTCACTCTGTGTCCCACACATACA TACATCTGTAATACAAAAGCCAAATCATGTGACATGGCACAAAATAGAACTTTGGTGCCAGTGATAGGAA CATGCAGCCTGAGCATGCGCCAGGGTGGTCAGCGCAGATCCTTAGCTGAGAGGTGAGGGGCCTGAAACTC AGGTCCTATTGGCCCAGGGTTTTTAGCTAATACTACCAAATGTTGGAAAATATACACCGTATACCTGCTG TTTCTAATTGAGGACTTTGTAAGAAATAATAGATCATGCTCCTGAGTGACATTTATTCAGTGTATTGCAC TTACTAGCTTTCAATTACACTTTACTGCAATGATGAAATATTTTGGAATCTTTTGTACAACTGTCAATAA ACGTACTATTTTTACTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4458562 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4458562 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14094 same embryo
	EMAGE:14090 same embryo
	EMAGE:14093 same embryo
	EMAGE:14091 same embryo
	EurExpress:euxassay_001737 same experiment
	MGI:4827132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source