EMAGE mouse gene expression spatial database

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EMAGE:14098

Rlbp1 retinaldehyde binding protein 1 ( MGI:97930)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001776. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001776_01	euxassay_001776_02	euxassay_001776_03	euxassay_001776_04

euxassay_001776_05	euxassay_001776_06	euxassay_001776_07	euxassay_001776_08	euxassay_001776_09

euxassay_001776_10	euxassay_001776_11	euxassay_001776_12	euxassay_001776_13	euxassay_001776_14

euxassay_001776_15	euxassay_001776_16	euxassay_001776_17	euxassay_001776_18	euxassay_001776_19

euxassay_001776_20	euxassay_001776_21	euxassay_001776_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14098_wholemount_strong.wlz

14098_wholemount_moderate.wlz

14098_wholemount_weak.wlz

14098_wholemount_possible.wlz

14098_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14098_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 14 15 20 moderate expression: see section 03 04

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 15 16 17

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 13

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 13 14

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 20

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 14

orbito-sphenoid

weak

regional

weak expression: see section 05 06 07 08 13 18 19 20

viscerocranium

weak

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4146
Entity Detected:	Rlbp1, retinaldehyde binding protein 1 ( MGI:97930)
Sequence:	sense strand is shown >T4146 GAAAGAGCTGTCAGGGACGGTCAAGACTCGGATGAAATCACACAACTTGCAAGTGTGAGAGACAGCATTG CAATCCAGGCCCCACACCCTGCTGCCCCAAGCTGCTTCTTGGTGTCTACAGAAGTCACAACTTAGTGTCT TCAAGAGGCAGTATGTCAGACGGGGTGGGCACTTTCCGCATGGTTCCTGAAGAGGAGCAGGAGCTCCGAG CACAACTGGAGCAGCTCACAACCAAGGATCATGGTCCTGTCTTTGGCCCATGCAGCCAGCTGCCCCGCCA CACTTTGCAGAAGGCCAAGGATGAGCTGAATGAAAAGGAGGAGACCCGGGAGGAAGCGGTGAGGGAGCTA CAGGAGCTGGTACAGGCACAGGCAGCTTCTGGCGAGGAATTGGCCCTGGCAGTGGCTGAGAGGGTGCAGG CAAGAGACAGCGCCTTCCTCCTGCGCTTCATCCGTGCCCGCAAGTTCGATGTGGGTCGTGCTTATGAGCT GCTCAAAGGCTATGTGAACTTCCGCCTCCAGTACCCTGAACTCTTCGATAGTCTCTCCATGGAGGCTCTC CGCTGCACTATCGAGGCCGGATACCCTGGTGTCCTTTCCAGTCGGGACAAGTATGGTCGAGTGGTTATGC TCTTCAACATCGAAAACTGGCACTGTGAAGAAGTGACCTTTGATGAGATCTTACAGGCATATTGTTTCAT TTTGGAGAAACTGCTGGAAAATGAGGAAACCCAAATCAACGGCTTCTGTATTGTTGAGAACTTCAAGGGC TTCACCATGCAGCAGGCAGCAGGGCTCCGCCCCTCGGATCTCAAGAAGATGGTGGACATGCTCCAGGATT CATTCCCAGCCAGGTTCAAAGCTATCCACTTCATCCACCAGCCATGGTACTTCACCACCACCTATAATGT GGTCAAGCCCTTCTTGAAGAACAAGCTGCTACAGAGGGTCTTTGTTCACGGAGATGACCTGGATGGCTTC TTCCAGGAGATTGATGAGAACATCCTGCCTGCTGACTTTGGGGGTACACTGCCCAAGTACGACGGCAAAG TTGTTGCTGAGCAGCTCTTTGGTCCCCGGGCTGAAGTTGAGAACACAGCCTTATGAGGAGATGCCCTGCC ATGGGATCCGTATTAGCATCCCAGTCCTTCTTCAGCTTTCCTGAACCCAAGTCCGGGGACAGGAATGCCT GAAGGGACTGTTTTCCCAGACTTCCCTAGGTTTAGGAACGCTCTAGTGGCACCTCATCCCAAACCGACCT AGAGGGCAGTGAGGCAGGGGAATGCCTTTCATCGGGAGATATGTGGGGCAGTTAAATTCCCACCTGCCTC TGCAGTTACAGGGCAGTGACTCTGAGAGAAGTTGGATTTCAAAGACTAATTAGGCTTCTCCGTGATTTCC TTTGTAATCGCTTTGATACTTAGGGCGTGGAAGGCAGAGTTAAAGGCATACTTGCAATTCAGTAAAAAGA AGAAAGAGAGGGGCTTGAAGCTCTGATGATCTTAACAGCAGTAGGCAGATAAGGGGTTTCCCACCCAGGA TTCCAGGGAGGGGCCAGCAACCTTAGCACAGTTGCTGATGAATACTCTAGTAGCTAAGAGCTCTGGACTC TGTCCAGGGTGGAGGTCATGTCCCTTCCTCTCCACCCATCTTGGATGTGATCCTTGGTGCTGGGGCCCCC ATTCACTAGATGCTGTAGGCTTTGTTGTATGGTTTTACTTAAATGTACCTTTGACATCTTCCCCGAACAC TCTCAGTTCTTATTAGGCAAATAAAACCGTTCTTTGTCTTGTGTCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481973 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481973 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14096 same embryo
	EMAGE:14095 same embryo
	EMAGE:14097 same embryo
	EurExpress:euxassay_001776 same experiment
	MGI:4827751 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source