EMAGE mouse gene expression spatial database

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EMAGE:14281

Foxq1 forkhead box Q1 ( MGI:1298228)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004083. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004083_01	euxassay_004083_02	euxassay_004083_03	euxassay_004083_04

euxassay_004083_05	euxassay_004083_06	euxassay_004083_07	euxassay_004083_08	euxassay_004083_09

euxassay_004083_10	euxassay_004083_11	euxassay_004083_12	euxassay_004083_13	euxassay_004083_14

euxassay_004083_15	euxassay_004083_16	euxassay_004083_17	euxassay_004083_18	euxassay_004083_19

euxassay_004083_20	euxassay_004083_21	euxassay_004083_22	euxassay_004083_23	euxassay_004083_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14281_wholemount_strong.wlz

14281_wholemount_moderate.wlz

14281_wholemount_weak.wlz

14281_wholemount_possible.wlz

14281_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14281_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 1 phalanx

moderate

regional

moderate expression: see section 07 08 09 20

forelimb digit 2 phalanx

moderate

regional

moderate expression: see section 08 09 10 20

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 08 09

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 07

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 07 moderate expression: see section 08

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 07 moderate expression: see section 08

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 07 moderate expression: see section 08

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 07 moderate expression: see section 08

submandibular gland primordium

strong

regional

strong expression: see section 17 19 weak expression: see section 07 08 09 10 16 18

naris

moderate

regional

moderate expression: see section 12 13 15 16 17

stomach

strong

regional

strong expression: see section 02 03 04 05 moderate expression: see section 06 07 08 09 10

kidney calyx

strong

regional

strong expression: see section 07 08 16 moderate expression: see section 17 18

genital tubercle of male

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5597
Entity Detected:	Foxq1, forkhead box Q1 ( MGI:1298228)
Sequence:	sense strand is shown >T5597 AAGACGTCCTATCCTCAAAAGTAGCGGGTGCGCCAGCAGACGTCAGGTCGGTGCCTGAGACCTAGACATC CAGCGAAGGAACACTTTTGGACGCCCAGACCGATAGCATAAGCGCCGGCTCGGTCCTCCCAGCACGAAAT CCTAAGGCTGGTGTGTCCTGGCCCCCGCTTCGCAGGGTGGCCCCTCATTCCTCGCTTGTCTTCCGCTCAA AGTCTCTAAACCTCGGAGTAAGGACAGCACTGCACCAACTTCTATCACTCCACTCCTCACTTCACCCAAC AATCAAAACCCCTCCCAAAAAAAATCCCAGCGGAGGAGAACCTTTCGGAAAAGCGTCTCTCTCGGAAAGA CGCAGGAGCGGTCCCTCCCAGGAGAAGACAGACTCAGCGGGCCACGGGATCGCCACTCCGGGCGCCACTC GCTTCCTACGACCTCGCAGGAAAGGGGTAACTGTGCCGGACTGGAAGCACCAGCGCCGGCATGAAATTGG AGGTGTTCGTCCCACGCGCAGCCCACGGGGACAAAATGGGCAGCGATCTGGAGGGGGCCGGCAGCAGCGA CGTGCCATCTCCACTGTCCGCGGCTGGTGACGACTCCTTAGGCTCAGACGGGGACTGTGCAGCCAACAGC CCGGCGGCGGGCAGCGGCGCCGGGGATCTGGAAGGTGGCGGCGGCGAGAGGAATTCGAGTGGCGGGCCGA GCGCCCAAGACGGTCCGGAGGCAACTGATGACAGCAGAACGCAGGCCTCCGCGGCAGGGCCGTGCGCGGG CGGCGTGGGCGGCGGCGAGGGCGCGCGCAGCAAGCCGTACACGCGGCGGCCCAAGCCCCCATACTCCTAC ATCGCTCTCATCGCCATGGCCATCCGCGACTCCGCGGGCGGACGCCTGACACTGGCCGAGATCAACGAGT ACCTCATGGGCAAGTTCCCCTTTTTCCGGGGCAGCTACACGGGCTGGCGCAACTCCGTGCGCCACAACCT CTCGCTCAACGACTGTTTCGTCAAGGTGCTGCGCGACCCCTCGCGGCCCTGGGGCAAGGACAACTACTGG ATGCTCAACCCCAACAGCGAATACACCTTCGCCGACGGGGTCTTCCGCCGCCGCCGCAAGCGCCTCAGCC ACCGGACCACAGTCTCCGCGTCCGGGCTGCGGCCGGAGGAAGCCCCACCCGGACCTGCCGGGACCCCGCA GCCCGCGCCCGCCGCCCGCTCCTCCCCGATCGCGCGCTCGCCGGCTCGCCAGGAGGAGCGCTCCAGCCCT GCGAGCAAGTTCTCCAGCTCCTTCGCCATCGACAGCATTCTCAGCAAGCCTTTTCGCAGCCGCCGCGACG GCGACTCGGCTCTGGGGGTGCAGCTACCCTGGGGCGCCGCTCCCTGCCCGCCGCTGCGCGCCTATCCCGC GCTCCTTCCCGCGGCGCCCGGTGGCGCTCTGCTACCGCTCTGTGCTTACGGCGCAAGCGAGCCTACGCTG CTGGCGTCGCGCGGGACCGAGGTGCAGCCCGCGGCGCCCCTTCTGCTGGCGCCCCTCTCCACCGCGGCTC CAGCCAAGCCATTCCGAGGTCCGGAGACCGCCGGCGCGGCGCACCTGTACTGCCCCCTACGGCTGCCCAC GGCCCTGCAGGCGGCAGCGGCCTGCGGTCCCGGTCCGCACCTGTCCTACCCGGTGGAGACTCTGCTAGCT TGACAGGAGCTCTGTGCTCAGCAGAAGGGGTGCTGAGCAGGGTGGAGTTGGGGGCGCCTTTTGTTGAGGA GGTTGGAATGTGGGCTCTAGGGCTGTGCACTTTCCACTTTCCGGAGGAATAGAGTTTTTCTTTTTTTCAA CTCTCCATCAAATGTGCCTTAAAGTTAAGCATTTTGACCCAAGTGTTTTAACTTAGTCCAGAATAGTTTC CTTTGTCTTTTGTAACTTTACAGAGGATCAAAGCCGTTCTTTTCCATTTCTTGGCCAGGCCTGTACTCTC CCGCATAGAGGCTTTCCTTTCCCTCCTATTTGCGTCACGCCTTCCACTGATTTACCTTCCCCACCCATAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6489152 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6489152 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14279 same embryo
	EMAGE:14282 same embryo
	EMAGE:14280 same embryo
	EMAGE:14283 same embryo
	EMAGE:14278 same embryo
	EurExpress:euxassay_004083 same experiment
	MGI:4824918 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source