EMAGE mouse gene expression spatial database

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EMAGE:14282

Car2 carbonic anhydrase 2 ( MGI:88269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004081. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004081_01	euxassay_004081_02	euxassay_004081_03	euxassay_004081_04

euxassay_004081_05	euxassay_004081_06	euxassay_004081_07	euxassay_004081_08	euxassay_004081_09

euxassay_004081_10	euxassay_004081_11	euxassay_004081_12	euxassay_004081_13	euxassay_004081_14

euxassay_004081_15	euxassay_004081_16	euxassay_004081_17	euxassay_004081_18	euxassay_004081_19

euxassay_004081_20	euxassay_004081_21	euxassay_004081_22	euxassay_004081_23	euxassay_004081_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14282_wholemount_strong.wlz

14282_wholemount_moderate.wlz

14282_wholemount_weak.wlz

14282_wholemount_possible.wlz

14282_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14282_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 16 17 18 19

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

cochlear duct

strong

regional

strong expression: see section 04 05 06 07 08 16 17 18 19 20

rectum

strong

regional

strong expression: see section 13

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 11 12 15

lower jaw molar

weak

regional

weak expression: see section 07 08

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

upper jaw molar

weak

regional

weak expression: see section 07 08

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24 moderate expression: see section 21 22 23

bladder

moderate

regional

moderate expression: see section 12

bladder

moderate

regional

Expression in the fundus region.

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20

rectum lumen

strong

regional

strong expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5576
Entity Detected:	Car2, carbonic anhydrase 2 ( MGI:88269)
Sequence:	sense strand is shown >T5576 GCTAGACGGACGACAACTTCTGCTCTGCCCCAATCACCGGCGTGACCATGTCCCACCACTGGGGATACAG CAAGCACAACGGACCAGAGAACTGGCACAAGGACTTCCCCATTGCCAATGGAGACCGGCAGTCCCCTGTG GATATTGACACAGCAACTGCCCACCATGACCCTGCCCTACAGCCTCTGCTCATATCTTATGATAAAGCTG CGTCCAAGAGCATTGTCAACAACGGCCACTCCTTTAACGTTGAGTTTGATGACTCTCAGGACAATGCAGT GCTGAAAGGAGGACCCCTCAGTGACTCCTACAGATTGATCCAGTTTCACTTTCACTGGGGCTCATCTGAT GGCCAGGGCTCTGAGCACACTGTGAACAAAAAAAAATATGCTGCAGAGCTTCACTTGGTTCACTGGAACA CCAAATATGGGGACTTTGGAAAAGCTGTGCAGCAACCGGATGGATTGGCTGTTTTGGGTATTTTTTTGAA GATTGGACCTGCCTCACAAGGCCTTCAGAAAGTCCTTGAAGCACTGCATTCCATTAAAACAAAGGGGAAG CGTGCGGCCTTTGCTAACTTCGATCCTTGCTCCCTTCTTCCTGGAAACTTGGACTACTGGACATACCCTG GCTCTCTGACCACTCCGCCTCTGCTGGAATGTGTGACCTGGATCGTGCTCAGGGAGCCCATTACTGTCAG CAGCGAGCAGATGTCTCATTTCCGTACGCTGAACTTCAATGAGGAGGGGGATGCTGAAGAAGCGATGGTG GACAACTGGCGTCCAGCTCAGCCGCTAAAGAATAGAAAGATCAAAGCGTCCTTTAAGTAAAACAACCCTG CAGCAGGGGTCCGAAGGGCACAAGTGTGACCGCCTCTCTGTAGCTAAGCGCAGTTAGGCTGGGTGATTTG GATCCCGACTCGCATCTGGTATTGTAGACCTTTTACCTCTCATCCGTTGTGCTTACTAACAAAATGTGAA AAGCAAGACCCAGGTGTCTCATGTGGTGGCAGCACAGTGGCAGGCCAGTGGTCAACTTAGGGCATCTTTT CTCTGCCACGGCAGCGCAATGCAAAGAGCAGACATGGCCTCTTGCTTCTCTTCACAGCCATAGGATAATG AATACTCAGGCCTGTTTGTTAAAATGCTATTTTTAAAACCATATGAAGGTAGGATAATTAATTACAAGTC CACATCATGAGACAAACTGAAGTAACTTAGGCAAAACAGGTAAAACAGTCATAGTTTTGTGATTATAAAT GAGATGAATGTTCACCCTTCCAAGATCTTATATTAAAGAAAAAATTTTAAAAAGCTTATATATTTGTAGC AAAGTTATTCTTAAATATGAATTATGTTATAACTTAGTGACTTTTGATTTCTAGAGGTGTAAATGAGGAT GTAAAAATTGATATAGTTGTGATACAGAGTATATTTCCCTTCAGATAACATACCACAACACAATGGATAA TGTATTTTAGATATATTCTCTAATAAAATTGAGAACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6479187 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6479187 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14279 same embryo
	EMAGE:14281 same embryo
	EMAGE:14280 same embryo
	EMAGE:14283 same embryo
	EMAGE:14278 same embryo
	EurExpress:euxassay_004081 same experiment
	MGI:4823630 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source