EMAGE mouse gene expression spatial database

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EMAGE:14306

Prox1 prospero-related homeobox 1 ( MGI:97772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004159. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004159_01	euxassay_004159_02	euxassay_004159_03	euxassay_004159_04

euxassay_004159_05	euxassay_004159_06	euxassay_004159_07	euxassay_004159_08	euxassay_004159_09

euxassay_004159_10	euxassay_004159_11	euxassay_004159_12	euxassay_004159_13	euxassay_004159_14

euxassay_004159_15	euxassay_004159_16	euxassay_004159_17	euxassay_004159_18	euxassay_004159_19

euxassay_004159_20	euxassay_004159_21	euxassay_004159_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14306_wholemount_strong.wlz

14306_wholemount_moderate.wlz

14306_wholemount_weak.wlz

14306_wholemount_possible.wlz

14306_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14306_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pancreas

strong

regional

strong expression: see section 08 09 10 13 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 12 13

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

rest of cerebellum mantle layer

strong

regional

strong expression: see section 04 05 moderate expression: see section 06 16 17

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 06

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 06 11 12

pons mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

pons marginal layer

moderate

regional

moderate expression: see section 06

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

strong

regional

strong expression: see section 08 09 10 12 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

spinal cord mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13

lens

strong

regional

strong expression: see section 01 02 22

cardiovascular system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

heart ventricle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5606
Entity Detected:	Prox1, prospero-related homeobox 1 ( MGI:97772)
Sequence:	sense strand is shown >T5606 GCTGAGAGAGCGCTGGGAAAGCGACGGCCACTGCAGCCGGGGAAGCCGCGCGGCGCACACAAGCCTCTCC GCCGTGCCCGCTGCTCCTCGGGCCACCGCTCCGCGCTCCAGCCCTCGCCGCCTTCTCCGCTCTCTCTCCC CTTCTTTCCCCGGGCTCCCGGCTCTGCCCTGCGCGCCTCTCCCCTCCCCTCCCTCCTTTCTCCCCTCGCC TCTCAGCACTCGCCCAGATCACTCGCTCCCGCACAGACTCACCGACCCCTGGGCCAATTATCACCAGTAA GACATCACCGCGTGCGCGCGTGGGTTTCTTCTCTGCCCGCAAGAGAAGAAGAGAGGGGGAGAGAGAGAGA GAGTGAAAAGAAAGAGAGAGAGAAAGAGAGAGAGTGGCTGCGCCCCGCTGCTGCCCGCACCGCGGTCCCG GGATTCTTGAGCTGTGCCCAGCTGACGAGCTTTTGAAGATGGCACAATAACTGTCCAGTGATGCCTGACC ATGACAGCACAGCCCTCTTAAGCCGGCAAACCAAGAGGAGAAGGGTTGACATTGGAGTGAAAAGGACGGT AGGGACAGCATCTGCATTTTTTGCTAAGGCAAGGGCAACATTTTTCAGTGCCATGAATCCCCAAGGTTCA GAGCAGGATGTTGAATATTCTGTGGTGCAACACGCAGATGGGGAAAAGTCGAACGTACTCCGCAAGCTGC TGAAGAGGGCGAACTCGTATGAAGATGCCATGATGCCTTTTCCAGGAGCAACTATAATTTCCCAGCTGTT GAAAAATAACATGAACAAAAACGGTGGCACCGAGCCCAGTTTCCAAGCCAGCGGACTCTCTAGCACAGGC TCCGAAGTACATCAGGAGGATATATGTAGCAACTCTTCAAGAGACAGCCCCCCAGAGTGTCTTTCCCCTT TTGGCAGGCCTACTATGAGCCAGTTTGATGTGGATCGCTTATGTGATGAGCACCTGAGAGCAAAGCGCGC CCGGGTTGAGAATATCATTCGGGGTATGAGCCATTCCCCCAGTGTGGCATTAAGGGGCAATGAAAACGAA AGAGAGATGGCCCCGCAGTCTGTGAGTCCCCGAGAAAGTTACAGAGAAAACAAACGCAAGCAGAAGCTGC CCCAGCAGCAGCAACAGAGTTTCCAGCAGCTGGTTTCAGCCCGAAAAGAACAGAAGCGAGAGGAGCGCCG ACAGCTGAAACAGCAGCTGGAAGACATGCAGAAGCAGCTGCGCCAGCTGCAGGAGAAGTTCTACCAGGTC TATGACAGCACAGACTCCGAAAATGATGAAGATGGCGACCTGTCTGAAGACAGCATGCGCTCGGAGATCC TGGATGCACGGGCCCAGGACTCGGTGGGGCGCTCAGACAATGAGATGTGTGAGCTGGACCCAGGGCAGTT CATCGACAGGGCCCGAGCCCTAATCAGGGAGCAGGAGATGGCTGAGAACAAGCCTAAGCGAGAAGGCAGC AACAAAGAAAGAGACCACGGGCCAAACTCCTTGCAGCCAGAAGGCAAGCATCTGGCAGAGACCTTAAAAC AGGAGCTGAACACGGCCATGTCGCAGGTTGTGGACACGGTGGTCAAAGTCTTCTCAGCCAAACCCTCTCG CCAGGTTCCTCAGGTCTTCCCACCTCTCCAGATCCCCCAGGCCAGATTCGCAGTCAACGGGGAAAACCAC AATTTCCACACGGCCAACCAGCGCCTGCAATGCTTTGGTGATGTCATCATTCCGAACCCCTTGGACACCT TTGGCAGTGTGCAGATGCCTAGTTCCACAGACCAGACGGAAGCCCTTCCCCTGGTGGTCCGAAAAAACTC ATCCGAGCAATCTGCCTCTGGCCCGGCCACTGGCGGCCACCACCAGCCCCTGCACCAGTCACCCCTCTCC GCCACTGCAGGCTTCACCACCCCTAGCTTCCGCCATCCCTTTCCCCTGCCCTTGATGGCTTATCCATTTC AGAGTCCACTAGGTGCTCCCTCCGGCTCCTTCTCGGGGAAGGACAGAGCCTCTCCTGAGTCCTTAGACTT GACTCGGGACACAACAAGTCTGAGGACCAAGATGTCATCACACCATCTGAGCCACCACCCCTGTTCACCA GCACACCCACCCAGCACCGCAGAAGGACTCTCTTTGTCACTCATAAAGTCTGAGTGTGGAGATCTTCAAG ATATGTCCGACATCTCACCTTATTCAGGAAGCGCAATGCAGGAAGGGCTATCACCCAATCACTTGAAAAA GGCAAAACTCATGTTCTTTTACACCCGCTACCCCAGCTCCAACATGCTGAAGACCTACTTCTCGGACGTG AAGTTCAACAGATGCATTACCTCGCAGCTCATCAAGTGGTTCAGCAATTTCCGTGAGTTTTACTATATCC AGATGGAGAAGTATGCGCGTCAAGCCATCAATGATGGAGTCACCAGTACAGAAGAGCTCTCCATCACCAG GGATTGTGAGCTATACCGAGCCCTCAACATGCACTACAACAAAGCAAATGACTTTGAGGTTCCAGAGAGA TTCCTGGAAGTTGCGCAGATCACGTTACGGGAGTTTTTCAATGCCATCATCGCGGGCAAAGATGTTGATC CTTCCTGGAAGAAGGCCATTTACAAGGTCATCTGCAAGCTGGATAGTGAAGTTCCTGAGATTTTCAAATC CCCTAACTGCCTACAAGAACTCCTTCACGAGTAGAGATTGCAACGCTCTTTTGAATGTATGGATAGAAGA ATTCCCCCTTGGATGTCCAACTTCTCTGTGTCTAGATTTTGGTTTTATATATTTGTGTGGGGGAGGCATG GATATGTTGTGATGTCAGCTGTTGGTTCCTGCTCATCTCTGGGTTCCTTTTCTTTTCCTTCATCACAAGG GGATGGGATGGGCGTTTTCCTTTAGTTTACTTTTGCCCAAGGCCCTTAATATTTGGAGACTTAAGGTAGG GTTGGTTTTCAGGAGAAGGGAATGTCAGCCTGTGTAAAGTGCACGTGAGCTATGGAGTGAGCCAGTGACT GTGACACAGCTCACATGCATACCCCATGCTGTGTCTCCTGTTTCTCTAGGGTTAAGAAGTTCTGGAAGAA CCCGAGGAAAACACTTCACACACTGGAATACCTTGTTTGATTTGAGCCTGATAAGCAAGCTTTTCTTAAA CATCCAAACCAATGCCCTCGGCAGCTGGGTAGCTCACATCTCAAAAGTATCCTCAAAAGACAGAGTTTTA GAAAGATTGTCACCAAGTCCTTTGTAAGAGAAATGGCATAAACCCCCAGACCCAAGTTTGTACAAAACCG ACTCTCCTGAGACAAGGCTGGGAACGCACGCAGGCCAACCTGCTGTCTCTGTGAACTTTAGAACAGGCTC GTGGTGAGATGTGAGCTTGGCCGTGTGGACTTAGAGGAAACCCTGTGCTTTGTCACTAGTTGCTATTTAC TTTGCTTCAGATATTAAAAGGCATGAACTTTCCAAATGTATCCTTTGCTTGACTCTCTTTATACGCTTAG CATTGCTGTTGCTGTTTAACCTTCTTTCTTCTCCTAAAACACTAATTTCTAGATACCTTTCTACAGTAAG GAATTAAGAAGTCCCACTGACTACGAAACCCTTGCCCACTCTTGGAGCACGCTGGCAGCTGCTAGTGGAC CTGATCTTTACTTCCTGTGAGGACATCACCCAGGAATGGCTCATTTGCATATGGGTATGGAGGGTGGCTG TCTGTCTTTTCCCTCTGGGTTGTAATATCTTAAAGCAGCTGACCAGGTCTCTTCTACTACAGAAGCTAAT ATTGACCCAAGTGTGTACAATAGGTTAGTTTGGGGTTACCCAAAAAGACACAACTCCAGCATACTGAGAA AGCCGACTTCTCTCTCTCCCACTCTCAGATGGCTCTTTCCAAACACCCAGGTTTTTGATAATGTACCACC TCCTACACGTGCATGCTAACTATCGCTCACGTTTGTTTTCTTAGCTGGATTTTTGTGTACGGGTGCAGTA AACAGCACTGTTCACTCTAAGAGTGTGATCCAGGCCCCTACTTCCCCTTAGACAATCTGATGTGCACACT GTAAAAATGACACAGCAACAGATCCAGTATTTAAAACATAAAGGACAGAAATGCCATTTCCCCTTAATTT CTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6490801 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6490801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14307 same embryo
	EMAGE:14304 same embryo
	EMAGE:14309 same embryo
	EMAGE:14308 same embryo
	EMAGE:14305 same embryo
	EurExpress:euxassay_004159 same experiment
	MGI:4827441 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source