EMAGE mouse gene expression spatial database

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EMAGE:14432

Ubash3b ubiquitin associated and SH3 domain containing, B ( MGI:1920078)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004224. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004224_01	euxassay_004224_02	euxassay_004224_03	euxassay_004224_04

euxassay_004224_05	euxassay_004224_06	euxassay_004224_07	euxassay_004224_08	euxassay_004224_09

euxassay_004224_10	euxassay_004224_11	euxassay_004224_12	euxassay_004224_13	euxassay_004224_14

euxassay_004224_15	euxassay_004224_16	euxassay_004224_17	euxassay_004224_18	euxassay_004224_19

euxassay_004224_20	euxassay_004224_21	euxassay_004224_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14432_wholemount_strong.wlz

14432_wholemount_moderate.wlz

14432_wholemount_weak.wlz

14432_wholemount_possible.wlz

14432_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14432_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

diencephalon

strong

regional

strong expression: see section 08 09 15 16 moderate expression: see section 10 14

vibrissa

moderate

spotted

moderate expression: see section 07 08 09 10

metencephalon

moderate

regional

moderate expression: see section 10

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 06 15 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 05 06 07 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15 16

retina

strong

regional

strong expression: see section 01 02 03 04

nasal cavity olfactory epithelium

moderate

spotted

moderate expression: see section 09 10 11 12 13 16 17 18 19

nasal cavity respiratory epithelium

moderate

spotted

moderate expression: see section 14 15

lower jaw incisor

moderate

spotted

moderate expression: see section 11 12 13 14 15

upper jaw incisor

moderate

spotted

moderate expression: see section 11 12 13 14 16 17 18

liver

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7902
Entity Detected:	Ubash3b, ubiquitin associated and SH3 domain containing, B ( MGI:1920078)
Sequence:	sense strand is shown >T7902 CACTGAGCGGGCACTCGGCACCGCGACCCCAGCCCTCGGCATGGCCGCGCGCGAGGAGCTGTACAGCAAA GTCACCCCGCGCAGGGACCGCCTGCAGCGCCCGGGCACAGTCAAGCATGGGTCGGCGCTGGACGTGCTCC TGTCCATGGGCTTTCCCCGCGCCCGCGCACAAAAAGCTCTGGCATCCACGGGAGGAAGAAGCGTTCAGGC AGCATGTGACTGGTTGTTCTCCCATGTGGGTGACCCCTTCCTGGATGACCCCCTGCCTCGGGAGTATGTC CTCTACCTCCGCCCGACGGGGCCATTAGCCCAGAAGCTCTCTGACTTCTGGCAGCAGTCCAAGCAGATCT GCGGGAAGAACAAGGCCCACAACATCTTCCCCCACATCACTCTCTGCCAGTTCTTCATGTGCGAGGACAG CAAGGTGGATGCTCTGGGGGAAGCCCTGCAGACTACTGTCAGTCGATGGAAATGTAAGTTCTCAGCCCCG CTGCCCCTGGAGCTGTACACCTCCTCCAACTTCATCGGGCTCTTCGTGAAGGAAGACAGCGCTGAAGTCC TCAAGAAGTTCGCGGCTGACTTTGCTGCAGAGGCTGCATCCAAAACAGAAGTGCATGTGGAGCCTCATAA GAAGCAGCTGCATGTGACGCTGGCCTACCACTTCCAAGCCAGCCACCTACCCACCCTAGAGAAGCTTGCC CAGAACATTGACGTCAAGCTAGGATGCGACTGGGTGGCCACCATATTCTCTCGGGATATCCGGTTTGCTA ACCATGAGACGTTACAGGTGATCTATCCCTATTCACCTCAGAATGATGACGAGCTGGAGCTGGTCCCAGG GGACTTCATCTTCATGTCTCCCATGGAACAGACCAGCACCAGTGAGGGCTGGATTTATGGCACCTCGCTG ACCACTGGCTGCTCTGGGCTGCTTCCTGAGAACTACATCACCAAGGCTGATGAGTGTAGCACCTGGATCT TCCATGGTTCCTACTCCATCCTAAATACCGTATCATCCAGCTCGCTCGCGTTTGGCGACGGAGCGTTAGA GAGGCGGCAGTATGAAGATCAAGGACTTGGGGAGACAACACCCCTCACTATCATCTGCCAGCCCATGCAG CCCCTGAGAGTCAACAGCCAGCCTGGACCCCAGAAGCGATGCCTCTTTGTATGTCGGCATGGGGAGAGGA TGGACGTTGTATTTGGGAAGTACTGGCTGTCCCAATGCTTTGATGCCAAAGGCCGCTACATACGCACCAA CCTGAACATGCCTCACAGTTTACCTCAGAGGAGCGGCGGTTTCCGGGATTACGAGAAAGATGCTCCAATC ACTGTGTTTGGGTGTATGCAAGCAAGACTAGTGGGTGAAGCCTTGTTAGAAAGTAATACTGTTATTGATC ACGTGTATTGCTCACCATCCCTCCGCTGCGTTCAGACTGCACATAATATATTGAAAGGTTTACAACAAGA CAATCACTTGAAGATTCGAGTGGAGCCGGGCTTATTTGAATGGACAAAGTGGGTTGCTGGGAGCACATTA CCGGCATGGATACCTCCATCAGAGTTAGCTGCAGCCAACCTGAGTGTTGATACAACCTACAGACCTCACA TTCCAGTCAGCAAATTGGCGATTTCGGAATCCTATGACACCTACATCAATAGGAGTTTCCAGGTGACGAA AGAGATAATAAGTGAATGCAAAAGTAAAGGAAATAACATTCTGATTGTGGCCCACGCATCTTCCCTCGAA GCGTGTACCTGTCAGCTCCAAGGTCTATCCCCGCAGAACTCAAAGGACTTCGTACAAATGGTCCGGAAGA TCCCATACCTAGGCTTCTGTTCCTGCGAAGAATTGGGAGAAACTGGAATATGGCAACTGACAGACCCGCC CATCCTTCCCCTTACCCATGGCCCCACTGGGGGCTTCAACTGGAGAGAGACCCTCCTGCAAGAATAAGCT GCCTGAGAGCTGGGAAGAACTGGCCTTTTGCAGATGAGTCCTCAGGATGCCCTGTAGCAGTGGGAAAGCC CACGCTGCATTCAAAGCAGACAGCTCAGAATCATTTAGCATATTTCTTTTCATGCTTAGGGGACGGTGAG ATTGTGTCAATGAGAGAAAGTCGTGATTCAGAGGGGAAATTAAAAAAAAAAAAAAAATCCATCTCTCTGG ACTTGCCTAGCTACCAAGGCTTTGGAGAAGTGTCTTCCTAGGCTGAGCACCTGATGCAGCAGAAACTACC ATCCTCCTTCAGGCTATGCATGGCTAAAGAGCCTCATTCTTCTCCCAGAGAGCTGCTCCCTCCCGGAGAG GCATGCAAGAGGAAGGACTGTGTCTGGAATCCAACTGGGGAGAGGATAGTCTTTTTGTACTCCATTTGCA AGTTAGGGAAAGCCACACTGAGGAGTGCTTTGATGGTTGTTAGGGACCATAAGAATAGTCCTGTGTCAGA CAGCAGATTCTACCCTCAGCAGACTCCATTCACTGCCGCTGTGAGGCACCCACTTGAGGTCTCCCGACAT TCACCCTGGTACCACACCACTTGATCTCCAGAGCAGTCTGGCCCTTCATGCTTCTATCTGTGAAATGGGG AGAACAGCCCTTTGCACGTTGAGATACTCAGGAGGGATCGAGAGAACGAGTGATGTGTGTTGAATGGGAG GTGGAGCTACTTGCAGAATTTTAACCAAGTTGAAATTACCCCTGTGATGGCCGGATGAACTGTGAAGTCC CCAAGCCTGCCTGAATCAGGACGTGTCTCTCCACCCAGACTCTAGACTGTGTTAAGCAGTTAAGGAGATT GACATTTGAAAGGATGTTATGTTGGATTCACTATTTGGATCAGCAAAAAACAAAAACAGAAAACAAAAAC ACAAAAAGAAAAAAAAAAACTCCAGTGCAATACAGATATTTTTAGAAACTATACCGAGGGTCGTGTTGTT GGTCACATACAGGCCACGTCCACTGTAGCAGAATGTCCATAGGTTAGCCCGTTTTAGGTTGTCCAGGAAG CCCAGGGACTAACGTGAATGGTGACAGTCACAACTGCACTGCTTCTTTTGCTGCCCTCTTTGCTTTTCTA TGGTAATTTAGAACAGACGGGAGGGAGGACCACTTGAAGGAAGCTGGAGAGTCAGAGCCTATCTGTGGGC TCTCACTGCACCTACTTCCTGTACTTAGAGAGAGTGTGTGTATTTAAAAGAAGAATAAATGACAATCAAA CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6510702 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6510702 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14433 same embryo
	EMAGE:14430 same embryo
	EMAGE:14435 same embryo
	EMAGE:14434 same embryo
	EMAGE:14431 same embryo
	EurExpress:euxassay_004224 same experiment
	MGI:4829048 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source